FastQCFastQC Report
Thu 2 Feb 2017
SRR4063971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1015172
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATAC4250.022.25851
TAGGACC8350.022.1335964
TTAGGAC8200.021.1948153
TAACGGC956.861774E-720.84282936
TTAACGG856.1100054E-620.70660235
TGTAGGA14800.020.6608432
TAGGACG12600.020.6049044
AGGACGT12600.020.4302865
GGACGTA550.004480947720.0016786
TATACAC5200.019.8843253
CTGTAGG14550.019.8072721
TTTAGGA9850.019.6535682
ACGTATG1352.5956979E-919.55623432
ATACACT4700.019.1931024
GGACGTG12750.019.1545496
GTAGGAC15400.018.9997233
GACGTGA7550.018.9419887
GTATCAA25000.018.5677321
AGGACCT11850.018.566965
ATAGGAC4400.018.4997313
Produced by FastQC (version 0.11.5)