FastQCFastQC Report
Thu 2 Feb 2017
SRR4063970_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063970_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457933
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64960.4455623132201548No Hit
TATCAACGCAGAGTACTTTTTTTTT41610.2854040617778732No Hit
GTACTTTTTTTTTTTTTTTTTTTTT35710.24493580980744656No Hit
GTACATGGGGTGGTATCAACGCAAA24630.1689378044121369No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23270.15960952938166567No Hit
GGTATCAACGCAGAGTACTTTTTTT22400.15364217697246718No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21230.14561711683595885No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG17420.11948422869912402No Hit
GAATAGGACCGCGGTTCTATTTTGT16940.11619189633542831No Hit
GATTAAGAGGGACGGCCGGGGGCAT16730.11475150092631144No Hit
GTATCTGATCGTCTTCGAACCTCCG16300.11180211985050068No Hit
GCGCAAGACGGACCAGAGCGAAAGC16250.1114591685626157No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15600.10700080182011107No Hit
ATCAGATACCGTCGTAGTTCCGACC15440.10590335769887917No Hit
GTATCAACGCAGAGTACATGGGGTG15440.10590335769887917No Hit
CTATTGGAGCTGGAATTACCGCGGC15160.10398283048672333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA755.8311343E-815.20282816
TCCAACG1250.014.44318318
CGTCTTA852.6925045E-713.4142615
TTATACC655.4600758E-513.1517794
TTACACC1051.9974323E-812.6646764
TCCTATA751.4829146E-512.6629382
ATACCGT3100.012.5625426
GCGTTAT1301.9826984E-1012.4224031
GCGCGCT1001.4386751E-712.352315
TTCTCGT1001.4425495E-712.34975411
CGTCGTA2850.012.33308810
TACCGTC3100.012.256987
CCGTCGT2900.012.1204499
CACGCGC951.0389995E-612.00058612
CGTTATT1353.7289283E-1011.9594422
ACCGTCG3050.011.8358318
CCAACGA1459.640644E-1111.79570319
GTCGTAG3000.011.71643411
GCCTTAG658.026541E-411.6916741
TTGCACC658.033477E-411.6904714
Produced by FastQC (version 0.11.5)