##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063970_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1457933
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.249555363655258	32.0	32.0	32.0	32.0	32.0
2	30.873260979756957	32.0	32.0	32.0	32.0	32.0
3	30.912804635055245	32.0	32.0	32.0	32.0	32.0
4	30.929869891140402	32.0	32.0	32.0	32.0	32.0
5	30.84037469485909	32.0	32.0	32.0	32.0	32.0
6	34.50326729691968	36.0	36.0	36.0	32.0	36.0
7	34.42746271605074	36.0	36.0	36.0	32.0	36.0
8	34.3872098374891	36.0	36.0	36.0	32.0	36.0
9	34.51100496387694	36.0	36.0	36.0	32.0	36.0
10	34.23209365588131	36.0	36.0	36.0	32.0	36.0
11	34.50547178779821	36.0	36.0	36.0	32.0	36.0
12	34.314015801823544	36.0	36.0	36.0	32.0	36.0
13	34.4051317858914	36.0	36.0	36.0	32.0	36.0
14	34.30954646063982	36.0	36.0	36.0	32.0	36.0
15	34.25278253527426	36.0	36.0	36.0	32.0	36.0
16	34.247613573463255	36.0	36.0	36.0	32.0	36.0
17	34.166679127230125	36.0	36.0	36.0	32.0	36.0
18	34.17080071580793	36.0	36.0	36.0	32.0	36.0
19	34.17229392571538	36.0	36.0	36.0	32.0	36.0
20	34.137442529937935	36.0	36.0	36.0	32.0	36.0
21	34.120898559810364	36.0	36.0	36.0	32.0	36.0
22	34.10145665130016	36.0	36.0	36.0	32.0	36.0
23	34.065864480740885	36.0	36.0	36.0	32.0	36.0
24	34.04910239359422	36.0	36.0	36.0	32.0	36.0
25	33.65501158146499	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	11.0
5	19.0
6	55.0
7	22.0
8	82.0
9	98.0
10	144.0
11	40.0
12	92.0
13	84.0
14	183.0
15	239.0
16	467.0
17	585.0
18	710.0
19	997.0
20	1477.0
21	2214.0
22	3437.0
23	5073.0
24	7523.0
25	11043.0
26	15811.0
27	20907.0
28	28246.0
29	38412.0
30	50560.0
31	68098.0
32	98246.0
33	138907.0
34	300316.0
35	663828.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.71434648895891	17.291237527223625	11.644162720070483	25.350253263746986
2	16.303714079149	19.91016957755831	38.244259728999666	25.541856614293025
3	18.974595000212634	24.640270745221557	28.30512613330663	28.08000812125918
4	12.438943584249108	15.54511264012709	36.00855492899587	36.00738884662793
5	14.52562520450294	36.90203683213713	33.03834605910401	15.533991904255926
6	33.45189723973622	35.5798735425043	17.402140187127458	13.56608903063202
7	29.999677551919284	30.530344765604255	20.808672344039753	18.661305338436705
8	28.219067733227938	32.60436093465459	19.522586205240312	19.653985126877153
9	27.838114191600326	14.15488608289871	18.69427669503157	39.31272303046939
10	16.345395610527103	26.243362567661144	30.923348594480593	26.487893227331156
11	37.01042410865944	21.22808065044132	23.005237104034514	18.756258136864723
12	25.64940435377343	23.420355266923774	28.62629710999877	22.303943269304025
13	29.41913689003402	19.586104749047177	25.663646964591997	25.331111396326804
14	23.91192888749786	19.993973749432893	25.31642963185649	30.777667731212755
15	24.977334386600898	27.38809089305605	22.720106955615204	24.914467764727842
16	25.272333547251268	25.720668933967993	24.452741867400622	24.554255651380114
17	23.444305498551135	25.792826639908522	25.593307353044125	25.169560508496215
18	24.4673643312408	24.803863354762093	27.07828647722865	23.650485836768457
19	25.483413942365114	24.372231138104343	25.94070946015516	24.20364545937538
20	25.858423603388385	24.04251959855568	25.403641006631243	24.695415791424686
21	27.086050438626597	23.771570955917685	24.74986614681292	24.3925124586428
22	26.019997693524292	24.1293740664265	25.39141441876812	24.459213821281082
23	24.24886472459918	24.22847610515585	25.95203525789544	25.570623912349532
24	24.62073888988042	25.029036642458024	25.8763849036917	24.47383956396985
25	24.790099693635824	24.50295621182032	25.88533618714918	24.821607907394682
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	472.0
1	472.0
2	732.0
3	992.0
4	992.0
5	992.0
6	2777.5
7	4563.0
8	4563.0
9	4563.0
10	4343.5
11	4124.0
12	4124.0
13	4124.0
14	3987.0
15	3850.0
16	3850.0
17	3850.0
18	6784.5
19	9719.0
20	9719.0
21	9719.0
22	16075.5
23	22432.0
24	22432.0
25	22432.0
26	34712.5
27	46993.0
28	46993.0
29	46993.0
30	59315.0
31	71637.0
32	71637.0
33	71637.0
34	82892.5
35	94148.0
36	94148.0
37	94148.0
38	104588.5
39	115029.0
40	115029.0
41	115029.0
42	131656.5
43	148284.0
44	148284.0
45	148284.0
46	166356.5
47	184429.0
48	184429.0
49	184429.0
50	189762.5
51	195096.0
52	195096.0
53	195096.0
54	178525.0
55	161954.0
56	161954.0
57	161954.0
58	149873.0
59	137792.0
60	137792.0
61	137792.0
62	122428.0
63	107064.0
64	107064.0
65	107064.0
66	88818.0
67	70572.0
68	70572.0
69	70572.0
70	53803.5
71	37035.0
72	37035.0
73	37035.0
74	28519.0
75	20003.0
76	20003.0
77	20003.0
78	16180.0
79	12357.0
80	12357.0
81	12357.0
82	8453.0
83	4549.0
84	4549.0
85	4549.0
86	3538.0
87	2527.0
88	2527.0
89	2527.0
90	1806.5
91	1086.0
92	1086.0
93	1086.0
94	748.0
95	410.0
96	410.0
97	410.0
98	613.0
99	816.0
100	816.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0383419539855398
2	0.005144269318274571
3	0.002126297984886823
4	0.004115415454619657
5	0.008916733485009256
6	0.0144039540911688
7	0.022909146030716088
8	0.03697014883399991
9	0.05027665880393681
10	0.0587132604859071
11	0.05692991378890525
12	0.06474920315268261
13	0.06488638366783658
14	0.06680691087999242
15	0.06035942666775497
16	0.06776717448607035
17	0.06385752980418168
18	0.08011342084992931
19	0.07592941513773267
20	0.08182817728935417
21	0.07634095668319463
22	0.07990765007719834
23	0.08491473888031892
24	0.0790845669862744
25	0.08025060136508332
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1457933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.849097941648395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.01898997782894	30.43099657276938
2	15.248683051018311	13.067846270684749
3	5.70216623608962	7.329980385892942
4	2.6505677437873545	4.542977474180734
5	1.4276479756033043	3.0586713966411025
6	0.8719147031322214	2.2416455106765505
7	0.5487489891269132	1.6459379426335754
8	0.3967382490723372	1.3599900873358937
9	0.3010315727197681	1.1609038208698015
>10	1.5766359385070798	12.252761335240669
>50	0.12280876673835112	3.6700179482656745
>100	0.11255191254485827	10.302716622613698
>500	0.015092530448572509	4.375140792570305
>1k	0.006261794547811999	4.113501951891234
>5k	1.6055883455928202E-4	0.4469118877336966
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6496	0.4455623132201548	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4161	0.2854040617778732	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3571	0.24493580980744656	No Hit
GTACATGGGGTGGTATCAACGCAAA	2463	0.1689378044121369	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2327	0.15960952938166567	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2240	0.15364217697246718	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2123	0.14561711683595885	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1742	0.11948422869912402	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1694	0.11619189633542831	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1673	0.11475150092631144	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1630	0.11180211985050068	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1625	0.1114591685626157	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1560	0.10700080182011107	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1544	0.10590335769887917	No Hit
GTATCAACGCAGAGTACATGGGGTG	1544	0.10590335769887917	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1516	0.10398283048672333	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.859025757699429E-5	0.0
6	0.0	0.0	0.0	6.859025757699429E-5	0.0
7	0.0	0.0	0.0	6.859025757699429E-5	0.0
8	0.0	0.0	0.0	6.859025757699429E-5	0.0
9	0.0	0.0	0.0	6.859025757699429E-5	0.0
10	0.0	0.0	0.0	6.859025757699429E-5	0.0
11	0.0	0.0	0.0	6.859025757699429E-5	0.0
12	0.0	0.0	0.0	6.859025757699429E-5	0.0
13	0.0	0.0	0.0	6.859025757699429E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCTA	75	5.8311343E-8	15.202828	16
TCCAACG	125	0.0	14.443183	18
CGTCTTA	85	2.6925045E-7	13.41426	15
TTATACC	65	5.4600758E-5	13.151779	4
TTACACC	105	1.9974323E-8	12.664676	4
TCCTATA	75	1.4829146E-5	12.662938	2
ATACCGT	310	0.0	12.562542	6
GCGTTAT	130	1.9826984E-10	12.422403	1
GCGCGCT	100	1.4386751E-7	12.3523	15
TTCTCGT	100	1.4425495E-7	12.349754	11
CGTCGTA	285	0.0	12.333088	10
TACCGTC	310	0.0	12.25698	7
CCGTCGT	290	0.0	12.120449	9
CACGCGC	95	1.0389995E-6	12.000586	12
CGTTATT	135	3.7289283E-10	11.959442	2
ACCGTCG	305	0.0	11.835831	8
CCAACGA	145	9.640644E-11	11.795703	19
GTCGTAG	300	0.0	11.716434	11
GCCTTAG	65	8.026541E-4	11.691674	1
TTGCACC	65	8.033477E-4	11.690471	4
>>END_MODULE