FastQCFastQC Report
Thu 2 Feb 2017
SRR4063970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457933
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19000.13032148939628913No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17380.11920986766881606No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA16990.11653484762331329No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT16590.11379123732023352No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15670.10748093362315002No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA15670.10748093362315002No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC15420.10576617718372518No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG14790.10144499095637455No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGG551.5940568E-423.99951418
AGTATCG602.8735652E-421.9995548
GTATCGA654.933506E-420.3072819
GTCGGTA550.004485463319.99890911
TAGGACG7200.019.8607084
GTACTAA808.9868554E-519.250931
GTATCAA48350.018.9750651
AATCGGT708.125526E-418.85676219
ACTATAC1051.7935527E-618.856763
GTCCTAA3650.018.6858351
CGCAATA6100.018.0330436
ACTAGAC1355.529182E-817.9255643
AATCACG3750.017.60024634
ACCGTAT750.001291218917.5996448
TATCGAG750.001291515917.5990410
AACGCAG52450.017.3228136
GGACGTG8650.017.0401156
AGGACGT8850.016.9036145
TAGCGGC6250.016.89623630
GATATAC4700.016.8518771
Produced by FastQC (version 0.11.5)