##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063970_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1457933
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24024766570206	32.0	32.0	32.0	32.0	32.0
2	31.389457540229902	32.0	32.0	32.0	32.0	32.0
3	31.4740869436387	32.0	32.0	32.0	32.0	32.0
4	31.56627567933506	32.0	32.0	32.0	32.0	32.0
5	31.498632653215203	32.0	32.0	32.0	32.0	32.0
6	35.08215329511027	36.0	36.0	36.0	36.0	36.0
7	35.11159772088292	36.0	36.0	36.0	36.0	36.0
8	35.05323152710035	36.0	36.0	36.0	36.0	36.0
9	35.16898238807956	36.0	36.0	36.0	36.0	36.0
10	35.01455348085268	36.0	36.0	36.0	36.0	36.0
11	35.16346361595492	36.0	36.0	36.0	36.0	36.0
12	35.06379031135176	36.0	36.0	36.0	36.0	36.0
13	35.10937265292712	36.0	36.0	36.0	36.0	36.0
14	35.06084916110685	36.0	36.0	36.0	36.0	36.0
15	35.03192945080467	36.0	36.0	36.0	36.0	36.0
16	35.039083414669946	36.0	36.0	36.0	36.0	36.0
17	34.996595865516454	36.0	36.0	36.0	36.0	36.0
18	34.99239196862956	36.0	36.0	36.0	36.0	36.0
19	34.991411813848785	36.0	36.0	36.0	36.0	36.0
20	34.97893593189811	36.0	36.0	36.0	36.0	36.0
21	34.97057477949947	36.0	36.0	36.0	36.0	36.0
22	34.96167587948143	36.0	36.0	36.0	36.0	36.0
23	34.90801429146607	36.0	36.0	36.0	32.0	36.0
24	34.878456005865836	36.0	36.0	36.0	32.0	36.0
25	34.849851810748504	36.0	36.0	36.0	32.0	36.0
26	34.795771822161925	36.0	36.0	36.0	32.0	36.0
27	34.7894032167459	36.0	36.0	36.0	32.0	36.0
28	34.76500771983349	36.0	36.0	36.0	32.0	36.0
29	34.73105279872257	36.0	36.0	36.0	32.0	36.0
30	34.70122083799461	36.0	36.0	36.0	32.0	36.0
31	34.69351266484811	36.0	36.0	36.0	32.0	36.0
32	34.64400147331873	36.0	36.0	36.0	32.0	36.0
33	34.61541854118125	36.0	36.0	36.0	32.0	36.0
34	34.591574509939754	36.0	36.0	36.0	32.0	36.0
35	34.54796276646458	36.0	36.0	36.0	32.0	36.0
36	34.51475342145353	36.0	36.0	36.0	32.0	36.0
37	34.49367014807951	36.0	36.0	36.0	32.0	36.0
38	34.44675646960457	36.0	36.0	36.0	32.0	36.0
39	34.434926707880265	36.0	36.0	36.0	32.0	36.0
40	34.407791030177656	36.0	36.0	36.0	32.0	36.0
41	34.38725442115653	36.0	36.0	36.0	32.0	36.0
42	34.30230607305	36.0	36.0	36.0	32.0	36.0
43	34.28355692614132	36.0	36.0	36.0	32.0	36.0
44	34.19853930187464	36.0	36.0	36.0	32.0	36.0
45	34.16187918100489	36.0	36.0	36.0	32.0	36.0
46	34.099074511654514	36.0	36.0	36.0	32.0	36.0
47	34.05010655496515	36.0	36.0	36.0	32.0	36.0
48	33.985646802699435	36.0	36.0	36.0	32.0	36.0
49	33.95894118591183	36.0	36.0	36.0	32.0	36.0
50	33.343886858998324	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	7.0
20	21.0
21	65.0
22	196.0
23	488.0
24	1385.0
25	3132.0
26	6164.0
27	11128.0
28	18419.0
29	27540.0
30	38559.0
31	54297.0
32	78886.0
33	125157.0
34	256808.0
35	835678.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.57218998961453	18.221766133491013	12.261165065859243	25.944878811035217
2	15.538215878973446	20.726400755044143	37.71509978544894	26.02028358053347
3	18.217578795097424	25.191796297071118	28.692794632378714	27.89783027545275
4	12.088964307687665	16.199509854019357	36.051107972725774	35.66041786556721
5	14.099619118299675	37.22825397326214	32.84883461722864	15.823292291209542
6	33.423118103668834	36.0632293297831	17.023645151931056	13.490007414617017
7	29.752347846127254	30.747524575906148	20.858037274715144	18.642090303251454
8	28.019002257992824	32.73232222078944	19.482390125191024	19.7662853960267
9	27.71510507727753	13.983621950893287	18.61142567494806	39.68984729688112
10	16.135911249024296	26.400795104264834	31.018600446388767	26.4446932003221
11	37.22537318244391	21.069555322501103	22.88150415691256	18.823567338142425
12	25.552667137654073	23.469851090244386	28.667562914543222	22.30991885755832
13	29.483740291879784	19.527768499014698	25.79823846017193	25.190252748933588
14	23.89104834179395	19.879404443694522	25.422396533169266	30.80715068134226
15	24.96023321615365	27.43513675726657	22.759015690645633	24.84561433593415
16	25.274294133301872	25.74969614328319	24.428365264581693	24.547644458833247
17	23.528241695606038	25.795012528010545	25.564617852809423	25.11212792357399
18	24.483702567134614	24.64722218983229	27.285004962518748	23.584070280514347
19	25.500005830171897	24.30228275236242	26.129115672668085	24.068595744797598
20	25.895845122348543	23.90116089647959	25.661333744877133	24.541660236294735
21	27.074083651306335	23.637094434380728	24.95841715634395	24.33040475796899
22	25.903214898373108	24.011767360732435	25.68201101423867	24.40300672665579
23	24.25326989148261	23.875746524336332	26.279851542228222	25.591132041952836
24	24.685940203975107	24.772364521308738	26.10213296586994	24.439562308846217
25	24.8600044996104	24.256261043251133	26.20282268242628	24.68091177471219
26	24.116796892952415	25.370563577659382	26.696148498436393	23.81649103095181
27	24.758821931564697	24.647493147414952	25.896529395463435	24.69715552555692
28	23.94903252756931	24.736756544303304	26.516955203855485	24.7972557242719
29	24.12207446753783	24.49260818836996	26.245338319510914	25.1399790245813
30	23.935058859681977	24.992420469807406	26.515665962902148	24.556854707608476
31	24.858814418386743	24.754412885426518	25.38884769380306	24.997925002383674
32	24.436028852933994	25.109817295846838	25.21318685539785	25.240966995821317
33	23.98765582836141	24.60081736579932	26.109404645458323	25.30212216038094
34	24.885879984498196	24.758847516453518	26.155793112672725	24.19947938637556
35	25.33502161684241	24.57351063968431	26.06888992386948	24.022577819603796
36	24.08555240015914	25.332757130509943	25.72313454335926	24.858555925971658
37	25.09671577907647	25.177380854391306	25.379866655691824	24.346036710840398
38	24.378485536523964	25.07377504613084	25.78580199065722	24.76193742668798
39	25.20481907876686	24.671576105446842	25.23479427549195	24.888810540294344
40	25.434255941438895	25.125866316938932	25.598396593446992	23.841481148175177
41	24.141705969989545	25.357159515245296	26.37993760854713	24.121196906218017
42	25.522255176595504	25.798884428921443	25.350913775080457	23.327946619402596
43	24.44312291524768	24.81214508195653	25.920446228737497	24.824285774058293
44	24.050145825388157	25.49694493754064	25.238492415159257	25.214416821911946
45	24.40785177706199	25.6833059882901	25.253581834843253	24.65526039980465
46	24.302289850141847	25.250769593775207	25.19397604244975	25.2529645136332
47	24.722770382472955	25.208672432466546	25.480912019765324	24.58764516529518
48	25.385588331726	25.573735408827204	24.423383739099062	24.617292520347732
49	24.45520099483992	25.896429372470287	24.782241586146867	24.866128046542922
50	24.605430483744627	26.176094951842284	24.43251266532137	24.785961899091717
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	204.0
1	139.0
2	74.0
3	197.0
4	320.0
5	370.5
6	421.0
7	393.5
8	366.0
9	549.5
10	733.0
11	1110.5
12	1488.0
13	2658.0
14	3828.0
15	5192.5
16	6557.0
17	7057.0
18	7557.0
19	7487.0
20	7417.0
21	7718.0
22	8019.0
23	8701.0
24	9383.0
25	9695.0
26	10007.0
27	12740.0
28	15473.0
29	17840.5
30	20208.0
31	23240.0
32	26272.0
33	30831.5
34	35391.0
35	40842.5
36	46294.0
37	51732.0
38	57170.0
39	61063.5
40	64957.0
41	68540.5
42	72124.0
43	72040.5
44	71957.0
45	79529.0
46	87101.0
47	93036.5
48	98972.0
49	102557.5
50	106143.0
51	102752.5
52	99362.0
53	99538.0
54	99714.0
55	101434.5
56	103155.0
57	100680.5
58	98206.0
59	89650.5
60	81095.0
61	72125.0
62	63155.0
63	55276.5
64	47398.0
65	40473.0
66	33548.0
67	28891.0
68	24234.0
69	21745.0
70	19256.0
71	14951.0
72	10646.0
73	9005.0
74	7364.0
75	5692.0
76	4020.0
77	3640.5
78	3261.0
79	2659.5
80	2058.0
81	1691.5
82	1325.0
83	1108.0
84	891.0
85	630.5
86	370.0
87	275.0
88	180.0
89	133.0
90	86.0
91	67.5
92	49.0
93	49.0
94	49.0
95	43.5
96	38.0
97	28.0
98	18.0
99	18.5
100	19.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008710962712278274
2	3.429512878849714E-4
3	4.115415454619657E-4
4	0.0
5	0.0
6	1.3718051515398854E-4
7	6.859025757699427E-5
8	6.859025757699427E-5
9	4.115415454619657E-4
10	7.544928333469371E-4
11	0.0
12	2.0577077273098286E-4
13	2.743610303079771E-4
14	1.3718051515398854E-4
15	0.003978234939465668
16	6.173123181929485E-4
17	0.0
18	2.743610303079771E-4
19	0.0
20	5.487220606159542E-4
21	0.0
22	1.3718051515398854E-4
23	6.859025757699429E-4
24	6.859025757699429E-4
25	0.001989117469732834
26	0.005898762151621508
27	0.006104532924352491
28	0.004252595969773646
29	0.002469249272771794
30	0.004046825197042663
31	0.006859025757699429
32	0.003909644681888674
33	0.005418630348582548
34	0.0026064297879257827
35	0.0028807908182337597
36	0.007750699106200354
37	0.003635283651580697
38	0.008436601681970296
39	0.004184005712196651
40	0.0025378395303487884
41	0.0028122005606567657
42	0.0017147564394248572
43	0.001783346697001851
44	0.0017147564394248572
45	0.0014403954091168798
46	0.0014403954091168798
47	0.001783346697001851
48	0.002126297984886823
49	6.859025757699429E-4
50	0.0010288538636549142
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1457933.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.26691554773169
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.71709466044629	34.37112772191726
2	14.797883945231868	13.989006614488186
3	5.303208629051202	7.519989432040954
4	2.4039343605894192	4.545062496170818
5	1.2885164092049821	3.0452098147879187
6	0.7850007212169611	2.2262737676802336
7	0.5187451275767118	1.7163637495176014
8	0.3645568917636154	1.3785183852267497
9	0.2511565572849957	1.0684256204204037
>10	1.3613401355910975	11.491965932854882
>50	0.10188734267477433	3.359003262349878
>100	0.09089317794257006	8.985481917684321
>500	0.011836531069253154	3.8281303425395388
>1k	0.0039455103564177176	2.4754409423213204
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1900	0.13032148939628913	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1738	0.11920986766881606	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1699	0.11653484762331329	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1659	0.11379123732023352	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1567	0.10748093362315002	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1567	0.10748093362315002	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1542	0.10576617718372518	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	1479	0.10144499095637455	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.859025757699429E-5	0.0
6	0.0	0.0	0.0	6.859025757699429E-5	0.0
7	0.0	0.0	0.0	6.859025757699429E-5	0.0
8	0.0	0.0	0.0	6.859025757699429E-5	0.0
9	0.0	0.0	0.0	6.859025757699429E-5	0.0
10	0.0	0.0	0.0	6.859025757699429E-5	0.0
11	0.0	0.0	0.0	6.859025757699429E-5	0.0
12	0.0	0.0	0.0	6.859025757699429E-5	0.0
13	0.0	0.0	0.0	6.859025757699429E-5	0.0
14	0.0	0.0	0.0	6.859025757699429E-5	0.0
15	0.0	0.0	0.0	6.859025757699429E-5	0.0
16	0.0	0.0	0.0	1.3718051515398857E-4	0.0
17	0.0	0.0	0.0	1.3718051515398857E-4	0.0
18	0.0	0.0	0.0	1.3718051515398857E-4	0.0
19	0.0	0.0	0.0	2.0577077273098283E-4	0.0
20	0.0	0.0	0.0	2.7436103030797714E-4	0.0
21	0.0	0.0	0.0	4.8013180303895994E-4	0.0
22	0.0	0.0	0.0	9.602636060779199E-4	0.0
23	0.0	0.0	0.0	0.002194888242463817	0.0
24	0.0	0.0	0.0	0.003772464166734685	0.0
25	0.0	0.0	0.0	0.0043211862273506395	0.0
26	0.0	0.0	0.0	0.005830171894044514	0.0
27	0.0	0.0	0.0	0.007407747818315382	0.0
28	0.0	0.0	0.0	0.010768670439588101	0.0
29	0.0	0.0	0.0	0.01666743259120961	0.0
30	0.0	0.0	0.0	0.028464956894452626	0.0
31	0.0	0.0	0.0	0.054460664516133454	0.0
32	0.0	0.0	0.0	0.08395447527424099	0.0
33	0.0	0.0	0.0	0.11056749521411478	0.0
34	0.0	0.0	0.0	0.1392382228812984	0.0
35	0.0	0.0	0.0	0.17312181012433356	0.0
36	0.0	0.0	0.0	0.22044908785245962	0.0
37	0.0	0.0	0.0	0.28924511620218485	0.0
38	0.0	0.0	0.0	0.38691764299182474	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATCGG	55	1.5940568E-4	23.999514	18
AGTATCG	60	2.8735652E-4	21.999554	8
GTATCGA	65	4.933506E-4	20.307281	9
GTCGGTA	55	0.0044854633	19.998909	11
TAGGACG	720	0.0	19.860708	4
GTACTAA	80	8.9868554E-5	19.25093	1
GTATCAA	4835	0.0	18.975065	1
AATCGGT	70	8.125526E-4	18.856762	19
ACTATAC	105	1.7935527E-6	18.85676	3
GTCCTAA	365	0.0	18.685835	1
CGCAATA	610	0.0	18.03304	36
ACTAGAC	135	5.529182E-8	17.925564	3
AATCACG	375	0.0	17.600246	34
ACCGTAT	75	0.0012912189	17.599644	8
TATCGAG	75	0.0012915159	17.59904	10
AACGCAG	5245	0.0	17.322813	6
GGACGTG	865	0.0	17.040115	6
AGGACGT	885	0.0	16.903614	5
TAGCGGC	625	0.0	16.896236	30
GATATAC	470	0.0	16.851877	1
>>END_MODULE