FastQCFastQC Report
Thu 2 Feb 2017
SRR4063969_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063969_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3910979
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT113020.28898135223942645No Hit
TATCAACGCAGAGTACTTTTTTTTT71940.18394371332599843No Hit
GCGCAAGACGGACCAGAGCGAAAGC42880.10964006710340302No Hit
GGTATCAACGCAGAGTACTTTTTTT42260.10805478628241165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.013.2274831
GCGTTAT2950.013.198741
GTATCAA63000.012.48131751
CGTCTTA2450.012.022634515
TACCGTA752.0747383E-411.400367
CAAGACG8800.011.3321064
CGTTATT2850.011.32716752
TCGCGTA4800.011.2823289
CGGACAT5800.011.1364315
AATTGCA6600.011.0849614
GCGTAAC5000.011.02049111
CGACCCG4250.010.9514665
CGCGTAA5050.010.91151610
GTATTAC1752.0008883E-1110.8533191
ATTTGCG4400.010.79745516
AAGACGG9400.010.7113245
CGACCAT6950.010.66248110
CTAAGAC2600.010.5920153
ACGGTAT5300.010.5764849
CGCCGGT7200.010.5558897
Produced by FastQC (version 0.11.5)