##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063969_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3910979
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244932279104543	32.0	32.0	32.0	32.0	32.0
2	30.872946390149373	32.0	32.0	32.0	32.0	32.0
3	30.88816406326907	32.0	32.0	32.0	32.0	32.0
4	30.91130481651781	32.0	32.0	32.0	32.0	32.0
5	30.842634542399743	32.0	32.0	32.0	32.0	32.0
6	34.48783846704367	36.0	36.0	36.0	32.0	36.0
7	34.42617206586893	36.0	36.0	36.0	32.0	36.0
8	34.38659271757788	36.0	36.0	36.0	32.0	36.0
9	34.50675572535675	36.0	36.0	36.0	32.0	36.0
10	34.23200968350891	36.0	36.0	36.0	32.0	36.0
11	34.482399419684945	36.0	36.0	36.0	32.0	36.0
12	34.3125560121903	36.0	36.0	36.0	32.0	36.0
13	34.38683434505785	36.0	36.0	36.0	32.0	36.0
14	34.28742649858258	36.0	36.0	36.0	32.0	36.0
15	34.22981611509548	36.0	36.0	36.0	32.0	36.0
16	34.23192070323057	36.0	36.0	36.0	32.0	36.0
17	34.15241247779648	36.0	36.0	36.0	32.0	36.0
18	34.147164431207635	36.0	36.0	36.0	32.0	36.0
19	34.170774887822205	36.0	36.0	36.0	32.0	36.0
20	34.14161594833416	36.0	36.0	36.0	32.0	36.0
21	34.12890046200708	36.0	36.0	36.0	32.0	36.0
22	34.109998800811766	36.0	36.0	36.0	32.0	36.0
23	34.05167376250294	36.0	36.0	36.0	32.0	36.0
24	34.048637438349836	36.0	36.0	36.0	32.0	36.0
25	33.65605005805452	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	15.0
4	32.0
5	42.0
6	150.0
7	58.0
8	258.0
9	244.0
10	344.0
11	107.0
12	257.0
13	184.0
14	356.0
15	675.0
16	1136.0
17	1535.0
18	2085.0
19	2897.0
20	4351.0
21	6126.0
22	9472.0
23	14604.0
24	21428.0
25	31085.0
26	43930.0
27	57625.0
28	77893.0
29	104204.0
30	136307.0
31	182109.0
32	255029.0
33	358811.0
34	779669.0
35	1817960.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.03647444946846	17.416367370440184	11.915541039159226	25.63161714093214
2	16.90461773553295	19.30986092686105	37.34924878821136	26.43627254939464
3	19.054553246262447	23.08307548646569	28.19239394995491	29.669977317316953
4	12.912865689075176	15.510059741702287	34.99937097848132	36.57770359074121
5	15.148665114489079	35.92168535289026	32.966078399032384	15.963571133588278
6	34.8178319868578	34.75310807742115	16.621923376711052	13.807136559009997
7	31.240586822203248	30.21405972312054	19.79792213810623	18.747431316569983
8	28.614980639992876	32.23217377914808	18.85977768603793	20.293067894821114
9	27.288166854647862	14.74945888788638	18.57642170956381	39.38595254790195
10	16.528817364790722	26.29593651350807	30.470712150753553	26.704533970947647
11	37.104449081213616	21.441180172592876	21.945983117293775	19.508387628899737
12	25.393804287450074	23.632402083983095	27.755619388319285	23.218174240247542
13	29.197159289633074	19.653602609110138	25.05447799247734	26.094760108779447
14	24.059459925548442	19.842678925664348	24.842662294447756	31.255198854339454
15	25.37567635815942	26.99333293592081	22.17508136509666	25.45590934082311
16	26.113405902412463	25.9454329786755	23.25410503821544	24.6870560806966
17	24.289963597586	25.889157361382736	24.656546242937246	25.164332798094012
18	24.80367699572131	25.045567100898598	25.887333407881783	24.263422495498304
19	25.68789944353934	24.75294216386355	25.423729680946693	24.135428711650412
20	26.040184098217107	24.150838529199977	24.76922470658259	25.039752666000325
21	27.195844068644924	24.072421740442106	24.396828804522354	24.334905386390616
22	26.256419284683968	23.986482942083974	24.912134261662953	24.844963511569105
23	24.76288206031077	24.12622576616783	25.41120744365058	25.69968472987082
24	24.82377702006794	24.949468714615623	25.10054439959314	25.126209865723297
25	24.970387007080248	24.483119250607675	25.20066337198397	25.3458303703281
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	245.0
1	245.0
2	700.0
3	1155.0
4	1155.0
5	1155.0
6	2810.5
7	4466.0
8	4466.0
9	4466.0
10	5030.0
11	5594.0
12	5594.0
13	5594.0
14	7250.5
15	8907.0
16	8907.0
17	8907.0
18	15816.0
19	22725.0
20	22725.0
21	22725.0
22	37578.0
23	52431.0
24	52431.0
25	52431.0
26	78824.5
27	105218.0
28	105218.0
29	105218.0
30	130902.0
31	156586.0
32	156586.0
33	156586.0
34	195953.0
35	235320.0
36	235320.0
37	235320.0
38	272263.0
39	309206.0
40	309206.0
41	309206.0
42	357247.0
43	405288.0
44	405288.0
45	405288.0
46	453303.5
47	501319.0
48	501319.0
49	501319.0
50	516798.0
51	532277.0
52	532277.0
53	532277.0
54	502800.0
55	473323.0
56	473323.0
57	473323.0
58	436588.0
59	399853.0
60	399853.0
61	399853.0
62	348880.0
63	297907.0
64	297907.0
65	297907.0
66	243733.0
67	189559.0
68	189559.0
69	189559.0
70	144468.5
71	99378.0
72	99378.0
73	99378.0
74	76643.0
75	53908.0
76	53908.0
77	53908.0
78	43228.5
79	32549.0
80	32549.0
81	32549.0
82	22044.5
83	11540.0
84	11540.0
85	11540.0
86	9005.5
87	6471.0
88	6471.0
89	6471.0
90	4590.5
91	2710.0
92	2710.0
93	2710.0
94	1908.0
95	1106.0
96	1106.0
97	1106.0
98	1522.0
99	1938.0
100	1938.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03912063961478699
2	0.005906449510467839
3	0.0016875570029908113
4	0.0036563735064800915
5	0.008795751651952106
6	0.013372610796427185
7	0.02306327904087442
8	0.03510629947130885
9	0.048581186449735476
10	0.057325800010687857
11	0.05438535977820387
12	0.06397375184065167
13	0.06443399465964916
14	0.06816707530262883
15	0.060394085470671155
16	0.06676077780013649
17	0.06256745433815931
18	0.0768605507725815
19	0.07379226531259821
20	0.07836912445707328
21	0.07481502713259262
22	0.07742306977357843
23	0.08230675746405183
24	0.07680941268158177
25	0.07839469350257314
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3910979.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.06872198842742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.11728582934049	22.765598352425915
2	16.272780184643057	11.738767689173622
3	7.469730559193523	8.082709046040351
4	3.9937805359389835	5.7620223933430355
5	2.4557111267365186	4.428718095707366
6	1.5909872537690735	3.4430926165997002
7	1.0569892919923902	2.6686977042332782
8	0.7556487965515282	2.1804229090965457
9	0.5434710342480086	1.7642075078749087
>10	2.4889962383602837	15.253299613926737
>50	0.1297003834706419	3.2164083147752125
>100	0.09765476515313878	7.376452178625444
>500	0.016629612948655847	4.164630126099598
>1k	0.010491644195487542	6.678833317626201
>5k	7.137172922100368E-5	0.18519421103206432
>10k+	7.137172922100368E-5	0.2909459234201266
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	11302	0.28898135223942645	No Hit
TATCAACGCAGAGTACTTTTTTTTT	7194	0.18394371332599843	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	4288	0.10964006710340302	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4226	0.10805478628241165	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.1138090999721556E-5
2	0.0	0.0	0.0	0.0	5.1138090999721556E-5
3	0.0	0.0	0.0	0.0	5.1138090999721556E-5
4	0.0	0.0	0.0	0.0	5.1138090999721556E-5
5	0.0	0.0	0.0	0.0	7.670713649958233E-5
6	0.0	0.0	0.0	0.0	1.7898331849902543E-4
7	0.0	0.0	0.0	0.0	1.7898331849902543E-4
8	0.0	0.0	0.0	0.0	1.7898331849902543E-4
9	0.0	0.0	0.0	0.0	1.7898331849902543E-4
10	0.0	0.0	0.0	0.0	1.7898331849902543E-4
11	0.0	0.0	0.0	0.0	1.7898331849902543E-4
12	0.0	0.0	0.0	0.0	3.8353568249791165E-4
13	0.0	0.0	0.0	0.0	4.0910472799777245E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2240	0.0	13.227483	1
GCGTTAT	295	0.0	13.19874	1
GTATCAA	6300	0.0	12.4813175	1
CGTCTTA	245	0.0	12.0226345	15
TACCGTA	75	2.0747383E-4	11.40036	7
CAAGACG	880	0.0	11.332106	4
CGTTATT	285	0.0	11.3271675	2
TCGCGTA	480	0.0	11.282328	9
CGGACAT	580	0.0	11.136431	5
AATTGCA	660	0.0	11.08496	14
GCGTAAC	500	0.0	11.020491	11
CGACCCG	425	0.0	10.951466	5
CGCGTAA	505	0.0	10.911516	10
GTATTAC	175	2.0008883E-11	10.853319	1
ATTTGCG	440	0.0	10.797455	16
AAGACGG	940	0.0	10.711324	5
CGACCAT	695	0.0	10.662481	10
CTAAGAC	260	0.0	10.592015	3
ACGGTAT	530	0.0	10.576484	9
CGCCGGT	720	0.0	10.555889	7
>>END_MODULE