FastQCFastQC Report
Thu 2 Feb 2017
SRR4063969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3910979
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT40660.10396373900243391No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70500.025.837661
GGTATCA29550.023.1534041
ATACCGT10700.018.9142116
CGTCGTA11100.017.83762610
ACCGTCG11300.017.7159258
TACCGTC11200.017.6774567
TCAACGC106200.017.1717364
TATCAAC107150.017.1426722
AACGCAG107300.017.0162016
ATCAACG107300.016.9956993
CGCAATA11300.016.74487736
CAACGCA109500.016.6743225
GTATAAT4500.016.621811
CCGTCGT12150.016.2958939
GTCGTAG12300.016.09736811
CGGTCCA12550.015.77670410
CGCCGGT12000.015.582357
TAGCGGC12400.015.43648430
CTAGCGG12300.015.38232429
CAATACG12950.014.61079938
Produced by FastQC (version 0.11.5)