##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063969_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3910979
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.365070740599734	32.0	32.0	32.0	32.0	32.0
2	31.448819592229977	32.0	32.0	32.0	32.0	32.0
3	31.502953096910005	32.0	32.0	32.0	32.0	32.0
4	31.577765311447592	32.0	32.0	32.0	32.0	32.0
5	31.53357918822883	32.0	32.0	32.0	32.0	32.0
6	35.1221259434019	36.0	36.0	36.0	36.0	36.0
7	35.135779558008366	36.0	36.0	36.0	36.0	36.0
8	35.08496798371968	36.0	36.0	36.0	36.0	36.0
9	35.188483241664045	36.0	36.0	36.0	36.0	36.0
10	35.04819534955314	36.0	36.0	36.0	36.0	36.0
11	35.181293737450396	36.0	36.0	36.0	36.0	36.0
12	35.08837633748481	36.0	36.0	36.0	36.0	36.0
13	35.12716125553218	36.0	36.0	36.0	36.0	36.0
14	35.068512001726425	36.0	36.0	36.0	36.0	36.0
15	35.0524359757493	36.0	36.0	36.0	36.0	36.0
16	35.060297690169136	36.0	36.0	36.0	36.0	36.0
17	35.01949460736046	36.0	36.0	36.0	36.0	36.0
18	35.014757174610246	36.0	36.0	36.0	36.0	36.0
19	35.01779937964382	36.0	36.0	36.0	36.0	36.0
20	35.00965691710439	36.0	36.0	36.0	36.0	36.0
21	35.00586298213312	36.0	36.0	36.0	36.0	36.0
22	34.994512627145276	36.0	36.0	36.0	36.0	36.0
23	34.940169456292146	36.0	36.0	36.0	36.0	36.0
24	34.91068144318852	36.0	36.0	36.0	32.0	36.0
25	34.88574727708842	36.0	36.0	36.0	32.0	36.0
26	34.824471570928914	36.0	36.0	36.0	32.0	36.0
27	34.82527316050534	36.0	36.0	36.0	32.0	36.0
28	34.802049052168265	36.0	36.0	36.0	32.0	36.0
29	34.77513430780375	36.0	36.0	36.0	32.0	36.0
30	34.76241089507256	36.0	36.0	36.0	32.0	36.0
31	34.755126785390566	36.0	36.0	36.0	32.0	36.0
32	34.71770853282516	36.0	36.0	36.0	32.0	36.0
33	34.69501856184858	36.0	36.0	36.0	32.0	36.0
34	34.688022615309364	36.0	36.0	36.0	32.0	36.0
35	34.64437906723611	36.0	36.0	36.0	32.0	36.0
36	34.629213554969226	36.0	36.0	36.0	32.0	36.0
37	34.62383254934378	36.0	36.0	36.0	32.0	36.0
38	34.57956153689396	36.0	36.0	36.0	32.0	36.0
39	34.58072186017874	36.0	36.0	36.0	32.0	36.0
40	34.55298430393004	36.0	36.0	36.0	32.0	36.0
41	34.539010820564364	36.0	36.0	36.0	32.0	36.0
42	34.47554691549098	36.0	36.0	36.0	32.0	36.0
43	34.46147831527605	36.0	36.0	36.0	32.0	36.0
44	34.394848706679326	36.0	36.0	36.0	32.0	36.0
45	34.36453890445333	36.0	36.0	36.0	32.0	36.0
46	34.334655338215825	36.0	36.0	36.0	32.0	36.0
47	34.30381037586753	36.0	36.0	36.0	32.0	36.0
48	34.26129595684354	36.0	36.0	36.0	32.0	36.0
49	34.238265150490456	36.0	36.0	36.0	32.0	36.0
50	33.64308067110562	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	5.0
18	2.0
19	12.0
20	45.0
21	144.0
22	452.0
23	1261.0
24	3191.0
25	7416.0
26	14590.0
27	26954.0
28	44145.0
29	67954.0
30	97317.0
31	137920.0
32	197016.0
33	311776.0
34	646833.0
35	2353945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08809619336868	18.274760824554043	12.491743642892706	26.14539933918458
2	16.119699511117474	19.969010166301878	36.89505901364371	27.016231308936938
3	18.291309987494124	23.59504479845235	28.49793183823618	29.61571337581735
4	12.556779261663129	16.11926323306773	35.01977382133732	36.304183683931825
5	14.700564743507954	36.39508164068383	32.73405456792277	16.170299047885454
6	34.861903965610615	35.06857882460993	16.375076899002654	13.694440310776804
7	31.067759591344764	30.38823576940169	19.822300020404125	18.721704618849422
8	28.597289834591287	32.46831547804271	18.64845093773196	20.285943749634043
9	27.370669265869964	14.594335584623858	18.451104618784935	39.58389053072124
10	16.449740816586917	26.5135950796446	30.502230028251542	26.534434075516945
11	37.281458171982	21.36286080799718	21.898864708810763	19.456816311210055
12	25.309724557175638	23.702503851341415	27.744283714419037	23.243487877063902
13	29.20311759294251	19.59538896528377	25.079072536043522	26.1224209057302
14	24.050164575666326	19.751330041905238	24.821099231980224	31.377406150448213
15	25.432222827379352	27.06704203455532	22.076822567076555	25.423912570988765
16	26.17006528069728	25.96262244765156	23.143491477917042	24.723820793734117
17	24.36522926868183	25.899269722491482	24.578705229560168	25.15679577926652
18	24.936473560267657	25.018345832363835	25.81443989598488	24.23074071138362
19	25.700560243642446	24.76662481011778	25.426664488182876	24.1061504580569
20	26.219581886860766	24.056399581585143	24.703428910257376	25.02058962129671
21	27.247806751199636	23.998645863350326	24.400463413380642	24.3530839720694
22	26.284105332382847	24.03246558656007	24.89355587306685	24.78987320799024
23	24.785090688916664	24.014025229662753	25.468044609087503	25.73283947233308
24	24.917028044265347	24.871182522961114	25.112555485098287	25.099233947675252
25	25.113631249718733	24.31869832386236	25.27970572472395	25.28796470169496
26	24.78722740508977	25.324055653803313	25.45965631127344	24.429060629833472
27	25.500897658722966	24.57112216162255	24.763976230518658	25.164003949135825
28	24.822831046725554	24.511744994329828	25.587684408492862	25.07773955045176
29	24.824808802133543	24.66571376701348	25.596146126053792	24.91333130479919
30	24.741397341635757	24.726029744100096	25.70119458140045	24.831378332863697
31	25.282175701232827	24.635204480852966	24.77157448671383	25.311045331200372
32	25.09651996280092	24.626289585713998	24.586988656427287	25.6902017950578
33	24.65333162183268	24.485207366885223	25.086235596929324	25.775225414352775
34	25.449587699486127	24.57065081065269	25.188366449229466	24.791395040631716
35	25.738986132223946	24.40585394791178	25.303225756961695	24.55193416290258
36	24.674546922777544	24.807694373030724	25.038650349732606	25.47910835445912
37	25.666528162216412	24.67571420352483	24.762396681007203	24.89536095325155
38	24.85966620604724	24.503666373793177	25.23277305509329	25.403894365066293
39	25.587796237797235	24.541614822974566	24.541026707108582	25.329562232119617
40	25.578002313543436	24.747959796301807	25.094837893427673	24.579199996727084
41	24.736705103579197	25.17945067331079	25.37613556631544	24.707708656794576
42	25.863507028539633	24.979927955423182	24.86772906166132	24.28883595437586
43	25.2517369922783	24.284242584942138	25.06272809489924	25.40129232788032
44	24.886727535487775	24.736456141822238	24.789742782012638	25.587073540677345
45	25.111910865076677	24.93126306436457	24.840005880961666	25.11682018959709
46	25.015878551835165	24.819710677955353	24.727558824792837	25.43685194541665
47	25.17508122812683	24.599433531778754	25.123226143145093	25.102259096949318
48	25.835177749185355	25.149811910494353	24.228439255987873	24.786571084332422
49	25.080178503441992	25.173812779007516	24.487127332642796	25.2588813849077
50	25.12435031387699	25.238696605689647	24.61495701658456	25.0219960638488
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	57.0
2	79.0
3	173.5
4	268.0
5	328.0
6	388.0
7	407.5
8	427.0
9	594.0
10	761.0
11	1225.5
12	1690.0
13	3320.0
14	4950.0
15	6921.5
16	8893.0
17	9493.0
18	10093.0
19	10286.5
20	10480.0
21	11305.5
22	12131.0
23	13627.0
24	15123.0
25	18455.5
26	21788.0
27	26838.5
28	31889.0
29	37471.0
30	43053.0
31	48520.0
32	53987.0
33	62413.5
34	70840.0
35	83862.5
36	96885.0
37	116732.5
38	136580.0
39	151083.5
40	165587.0
41	183923.5
42	202260.0
43	208005.5
44	213751.0
45	234211.0
46	254671.0
47	273151.5
48	291632.0
49	300787.0
50	309942.0
51	300382.5
52	290823.0
53	290598.5
54	290374.0
55	290869.0
56	291364.0
57	283360.0
58	275356.0
59	251610.5
60	227865.0
61	200821.0
62	173777.0
63	150463.5
64	127150.0
65	108399.5
66	89649.0
67	76697.0
68	63745.0
69	56462.5
70	49180.0
71	37829.5
72	26479.0
73	22509.5
74	18540.0
75	14105.5
76	9671.0
77	8413.0
78	7155.0
79	6061.0
80	4967.0
81	4019.0
82	3071.0
83	2503.5
84	1936.0
85	1355.0
86	774.0
87	557.0
88	340.0
89	261.0
90	182.0
91	149.0
92	116.0
93	94.5
94	73.0
95	88.5
96	104.0
97	77.5
98	51.0
99	52.5
100	54.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009102580197950436
2	4.858118644973548E-4
3	4.60242818997494E-4
4	0.0
5	0.0
6	1.2784522749930387E-4
7	1.2784522749930387E-4
8	0.0
9	9.460546834948487E-4
10	0.0010738999109941526
11	0.0
12	1.0227618199944311E-4
13	3.3239759149819006E-4
14	3.068285459983293E-4
15	0.003809787779479256
16	5.625190009969372E-4
17	0.0
18	2.30121409498747E-4
19	5.1138090999721556E-5
20	7.159332739961018E-4
21	0.0
22	1.7898331849902546E-4
23	6.90364228496241E-4
24	4.858118644973548E-4
25	0.0021222307764884444
26	0.005906449510467839
27	0.006392261374965195
28	0.004193323461977168
29	0.0027870259594848248
30	0.003912063961478699
31	0.007542868422458929
32	0.003323975914981901
33	0.0046279972354748
34	0.003017147368983572
35	0.0029148711869841286
36	0.007082625603461436
37	0.0039376330069785595
38	0.007747420786457815
39	0.004730273417474244
40	0.0024290593224867737
41	0.003963202052478421
42	0.0017131260484906721
43	0.0013295903659927603
44	0.0014062975024923426
45	0.001380728456992482
46	0.0010994689564940133
47	0.002045523639988862
48	0.0019176784124895583
49	4.60242818997494E-4
50	0.0013295903659927603
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3910979.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.63538196734681
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.4733356864328	24.90951950954304
2	15.974442308842955	12.343573606349862
3	7.160304160436387	8.299232587225271
4	3.894669021256816	6.018881010905993
5	2.277207981839114	4.399040009872256
6	1.4747062292001685	3.418550307286452
7	1.0137475018319086	2.7416565366203587
8	0.6979329765401509	2.157192570898882
9	0.5053149225962813	1.757073154347685
>10	2.3138148186059135	15.097792148884517
>50	0.11060214101997398	2.954489037896967
>100	0.08193599005330973	6.635654865683647
>500	0.013458256966770258	3.577384239162143
>1k	0.008528004377501763	5.689960415323053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	4066	0.10396373900243391	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.5569045499860778E-5
2	0.0	0.0	0.0	0.0	2.5569045499860778E-5
3	0.0	0.0	0.0	2.5569045499860778E-5	2.5569045499860778E-5
4	0.0	0.0	0.0	2.5569045499860778E-5	2.5569045499860778E-5
5	0.0	0.0	0.0	2.5569045499860778E-5	2.5569045499860778E-5
6	0.0	0.0	0.0	2.5569045499860778E-5	1.0227618199944311E-4
7	0.0	0.0	0.0	2.5569045499860778E-5	1.0227618199944311E-4
8	0.0	0.0	0.0	2.5569045499860778E-5	1.0227618199944311E-4
9	0.0	0.0	0.0	2.5569045499860778E-5	1.5341427299916466E-4
10	0.0	0.0	0.0	2.5569045499860778E-5	1.5341427299916466E-4
11	0.0	0.0	0.0	2.5569045499860778E-5	1.5341427299916466E-4
12	0.0	0.0	0.0	2.5569045499860778E-5	3.8353568249791165E-4
13	0.0	0.0	0.0	5.1138090999721556E-5	4.346737734976332E-4
14	0.0	0.0	0.0	5.1138090999721556E-5	5.113809099972156E-4
15	0.0	0.0	0.0	5.1138090999721556E-5	5.625190009969371E-4
16	0.0	0.0	0.0	5.1138090999721556E-5	6.136570919966586E-4
17	0.0	0.0	0.0	5.1138090999721556E-5	6.136570919966586E-4
18	0.0	0.0	0.0	5.1138090999721556E-5	6.647951829963802E-4
19	0.0	0.0	0.0	5.1138090999721556E-5	6.647951829963802E-4
20	0.0	0.0	0.0	7.670713649958233E-5	7.159332739961017E-4
21	0.0	0.0	0.0	7.670713649958233E-5	7.670713649958233E-4
22	0.0	0.0	0.0	7.670713649958233E-5	7.670713649958233E-4
23	2.5569045499860778E-5	0.0	0.0	1.7898331849902543E-4	8.437785014954056E-4
24	2.5569045499860778E-5	0.0	0.0	2.556904549986078E-4	8.437785014954056E-4
25	2.5569045499860778E-5	0.0	0.0	2.8125950049846853E-4	8.437785014954056E-4
26	2.5569045499860778E-5	0.0	0.0	3.323975914981901E-4	9.20485637994988E-4
27	2.5569045499860778E-5	0.0	0.0	3.8353568249791165E-4	9.460546834948487E-4
28	2.5569045499860778E-5	0.0	0.0	0.0010994689564940135	9.716237289947096E-4
29	2.5569045499860778E-5	0.0	0.0	0.0021477998219883053	9.716237289947096E-4
30	2.5569045499860778E-5	0.0	0.0	0.004372306780476193	9.716237289947096E-4
31	2.5569045499860778E-5	0.0	0.0	0.012426556112932337	9.971927744945703E-4
32	2.5569045499860778E-5	0.0	0.0	0.022168362448379293	0.0010483308654942918
33	2.5569045499860778E-5	0.0	0.0	0.03257496396682263	0.0010483308654942918
34	2.5569045499860778E-5	0.0	0.0	0.045257210534753574	0.0010994689564940135
35	2.5569045499860778E-5	0.0	0.0	0.06085432828966865	0.0010994689564940135
36	2.5569045499860778E-5	0.0	0.0	0.08504264533253694	0.0010994689564940135
37	2.5569045499860778E-5	0.0	0.0	0.12083930903234202	0.0010994689564940135
38	2.5569045499860778E-5	0.0	0.0	0.17358824989855481	0.001150607047493735
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7050	0.0	25.83766	1
GGTATCA	2955	0.0	23.153404	1
ATACCGT	1070	0.0	18.914211	6
CGTCGTA	1110	0.0	17.837626	10
ACCGTCG	1130	0.0	17.715925	8
TACCGTC	1120	0.0	17.677456	7
TCAACGC	10620	0.0	17.171736	4
TATCAAC	10715	0.0	17.142672	2
AACGCAG	10730	0.0	17.016201	6
ATCAACG	10730	0.0	16.995699	3
CGCAATA	1130	0.0	16.744877	36
CAACGCA	10950	0.0	16.674322	5
GTATAAT	450	0.0	16.62181	1
CCGTCGT	1215	0.0	16.295893	9
GTCGTAG	1230	0.0	16.097368	11
CGGTCCA	1255	0.0	15.776704	10
CGCCGGT	1200	0.0	15.58235	7
TAGCGGC	1240	0.0	15.436484	30
CTAGCGG	1230	0.0	15.382324	29
CAATACG	1295	0.0	14.610799	38
>>END_MODULE