FastQCFastQC Report
Thu 2 Feb 2017
SRR4063968_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063968_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196740
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT228211.0388575798683504No Hit
GTCCTACAGTGGACATTTCTAAATT204390.9304241740032958No Hit
CTTTAGGACGTGAAATATGGCGAGG200880.9144459517284703No Hit
CTGTAGGACGTGGAATATGGCAAGA197640.8996968234747854No Hit
GTCCTACAGTGTGCATTTCTCATTT192080.8743865910394494No Hit
TTGTAGAACAGTGTATATCAATGAG150500.6851061117838252No Hit
GTATCAACGCAGAGTACTTTTTTTT147680.6722689075630253No Hit
GATATACACTGTTCTACAAATCCCG146830.6683995374964721No Hit
CTGTAGGACCTGGAATATGGCGAGA134770.6135000045522001No Hit
TATCAACGCAGAGTACTTTTTTTTT110970.5051576426887114No Hit
GTGTATATCAATGAGTTACAATGAA109990.5006964866119795No Hit
ATTTAGAAATGTCCACTGTAGGACG94600.4306381274069758No Hit
CTGAAGGACCTGGAATATGGCGAGA88450.40264209692544406No Hit
GTGTATATCAATGAGTTACAATGAG85860.3908518987226527No Hit
GTCCTTCAGTGTGCATTTCTCATTT82160.3740087584329506No Hit
GATATACACTGTTCTACAATGCCGG81940.3730072744157251No Hit
GTACTTTTTTTTTTTTTTTTTTTTT81750.37214235640084853No Hit
GTACATGGGGTGGTATCAACGCAAA79380.36135364221528266No Hit
GTTCTACAGTGTGGTTTTTATCATT79190.36048872420040606No Hit
TTTCTAAATTTTCCACCTTTTTCAG71960.32757631763431266No Hit
GAATATGGCAAGAAAACTGAAAATC66810.3041324872310788No Hit
GTTCTACAAATCCCGTTTCCAACGA66310.30185638719192986No Hit
CTGTAGGACATGGAATATGGCAAGA60710.276364066753462No Hit
CTGTAGAACATATTAGATGAGTGAG58860.2679424966086109No Hit
ACGCAGAGTACTTTTTTTTTTTTTT57720.2627529885193514No Hit
GAGTACTTTTTTTTTTTTTTTTTTT56540.25738139242695995No Hit
GGAATATGGCGAGAAAACTGAAAAT54410.24768520626018553No Hit
GGTATCAACGCAGAGTACTTTTTTT53540.24372479219206644No Hit
ATGTTCTACAGTGTGGTTTTTATCA53370.2429509181787558No Hit
GTATCAACGCAGAGTACATGGGGTG48920.22269362783033042No Hit
GTAGAACAGTGTATATCAATGAGTT48770.2220107978185857No Hit
GAAATATGGCGAGGAAAACTGAAAA46110.20990194561031345No Hit
ATTCCAGGTCCTTCAGTGTGCATTT43960.2001147154419731No Hit
GTAGAACATATTAGATGAGTGAGTT43440.19774757140125823No Hit
GCCATATTCCACGTCCTACAGTGGA42890.1952438613581944No Hit
ACCTGGAATATGGCGAGAAAACTGA41520.18900734725092636No Hit
CCATATTCCAGGTCCTTCAGTGTGC41230.18768720922822No Hit
TTGATATACACTGTTCTACAAATCC40640.1850014111820243No Hit
TTCCAGGTCCTTCAGTGTGCATTTC39800.1811775631162541No Hit
CCATATTTCACGTCCTAAAGTGTGT38510.17530522501524987No Hit
GACCTGGAATATGGCGAGAAAACTG37670.17148137694947968No Hit
ACAGTGGACATTTCTAAATTTTCCA37360.17007019492520734No Hit
TATTAGATGAGTGAGTTACACTGAA36920.16806722689075632No Hit
CATCTAATATGTTCTACAGTGTGGT36070.16419785682420315No Hit
ATACACACTTTAGGACGTGAAATAT35300.1606926627639138No Hit
GTTCTACAATGCCGGTTTCCAACGT34920.15896282673416062No Hit
TATCAACGCAGAGTACATGGGGTGG34750.15818895272084998No Hit
GTCCACTGTAGGACGTGGAATATGG34550.15727851270519042No Hit
CACTTTAGGACGTGAAATATGGCGA33870.1541830166519479No Hit
CCTAAAGTGTGTATTTCTCATTTTC33160.1509509545963564No Hit
GTACATGGGTGGTATCAACGCAAAA32850.1495397725720841No Hit
CCACTGTAGGACGTGGAATATGGCA31380.14284803845698626No Hit
GAAATACACACTTTAGGACGTGAAA29880.13601973833953948No Hit
GTATATCAATGAGTTACAATGAAAA29870.1359742163387565No Hit
GTGTATTTCTCATTTTCCGTGATTT28770.1309667962526289No Hit
TCCTAAAGTGTGTATTTCTCATTTT28490.1296921802307055No Hit
CTACAGTGGACATTTCTAAATTTTC28210.12841756420878211No Hit
GCAGAGTACTTTTTTTTTTTTTTTT28180.12828099820643318No Hit
GTAGGACGTGGAATATGGCAAGAAA26350.11995047206314813No Hit
AGTGTGTATTTCTCATTTTCCGTGA26160.11908555404827152No Hit
GTGTGGTTTTTATCATTTTCCATGT26040.11853929003887578No Hit
CTGTTCTACAATGCCGGTTTCCAAC25830.11758332802243325No Hit
ATTCCACGTCCTACAGTGGACATTT25140.11444230996840773No Hit
GGTATCAACGCAGAGTACATGGGGT24880.1132587379480503No Hit
GTATATCAATGAGTTACAATGAGAA24830.11303112794413539No Hit
GAGTTACACTGAAAAACACATTCGT24350.11084607190655242No Hit
GTCCTACAGTGGACATTTCTAAATA24220.11025428589637372No Hit
ATATTTCACGTCCTAAAGTGTGTAT24210.11020876389559074No Hit
TTTCTAAATATTCCACCTTTTTCAG24040.10943488988228012No Hit
CTGTAGAACATTTTAGATGAGTGAG22830.10392672778753971No Hit
TTGATATACACTGTTCTACAATGCC22650.10310733177344611No Hit
GTATCAACGCAGAGTACATGGGAAA22020.10023944572411846No Hit
ACGCAGAGTACATGGGGTGGTATCA22010.10019392372333548No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGC350.002171852916.2842887
GTGCGTT951.8189894E-1116.0000611
ACGTGTA902.2901077E-914.7771588
TCGGTCG456.767222E-414.7768219
AGCACTC456.774238E-414.7748025
TTGTAGA23800.014.6053041
GACGTGT851.7198545E-814.5281397
CCAACGT6600.014.25427319
AGGCGAG1400.014.25394818
ACCGGAT400.005277514.25102612
CGGTCGA400.0052814914.24940310
TAGGCCG400.00528707914.2471315
TTTATAC400.00529107514.2455093
CCACCTT19650.014.215734513
TCCAACG20400.014.20736618
TAGAAAT27150.014.0634664
CCAACGA14200.013.98658719
CACCTTT20500.013.90470714
TACGGCG551.9568861E-413.82138116
AAATGTC27900.013.6869747
Produced by FastQC (version 0.11.5)