FastQCFastQC Report
Thu 2 Feb 2017
SRR4063968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196740
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA100930.4594535539026012No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG87260.3972249788322696No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA86240.3925817347524058No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA79480.3618088622231124No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC73000.33231060571574245No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA67010.30504292724673837No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC59520.2709469486602875No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC56250.2560612544042536No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA52760.24017407613099412No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA49990.22756448191410908No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA48860.22242049582563256No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA47680.21704889973324107No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA44560.2028460354889518No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA37670.17148137694947968No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA34920.15896282673416062No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA33050.15045021258774366No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32980.15013155858226282No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGTATGTGTTTTTCAGTG32460.14776441454154793No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT30800.1402077624115735No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA30730.13988910840609267No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA30340.1381137503755565No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACTC29580.13465407831605014No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG27580.12554967815945445No Hit
GTTCTACAGTGTGGTTTTTATCATTTTCCATGTTTTTCATTGTAACTCAT24130.10984458788932691No Hit
CTGTAGAACATATTAGATGAGTGAGTTACACTGAAAAACACATTCGTTGG23980.10916175787758224No Hit
TTGATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG23660.10770505385252693No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT23100.10515582180868013No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA22140.10078570973351421No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC51100.028.8010964
ACTGTGC3100.025.548448
GTCCTAT5650.024.528791
AGGACCT85500.024.520575
GGACCTG83800.024.2567026
TGTAGGA140850.023.5515022
CTGTAGG135650.023.4007951
ACTGTTC62450.023.3563338
CTCTAGG6700.023.311361
CCTAGAG5450.023.0071283
CTGTGCA3650.022.9041589
ATAGGAC6500.022.6749043
CTAGGAC7400.022.5925853
TAGGACA32400.022.3385354
AGGACCG3450.022.3178715
GATATAC67950.022.273221
GTGTAGG4750.022.2296031
TAGAGTG6850.022.1596185
ATACACT69550.022.014864
TCCGTTA1306.91216E-1122.0020526
Produced by FastQC (version 0.11.5)