##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063968_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2196740
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.221708531733388	32.0	32.0	32.0	32.0	32.0
2	31.51650991924397	32.0	32.0	32.0	32.0	32.0
3	31.55721978932418	32.0	32.0	32.0	32.0	32.0
4	31.634416453471964	32.0	32.0	32.0	32.0	32.0
5	31.59107495652649	32.0	32.0	32.0	32.0	32.0
6	35.18743092036381	36.0	36.0	36.0	36.0	36.0
7	35.222658575889724	36.0	36.0	36.0	36.0	36.0
8	35.17720986552801	36.0	36.0	36.0	36.0	36.0
9	35.269055964747764	36.0	36.0	36.0	36.0	36.0
10	35.15801232735781	36.0	36.0	36.0	36.0	36.0
11	35.25611815690523	36.0	36.0	36.0	36.0	36.0
12	35.21465398727205	36.0	36.0	36.0	36.0	36.0
13	35.234677294536446	36.0	36.0	36.0	36.0	36.0
14	35.181993772590296	36.0	36.0	36.0	36.0	36.0
15	35.16059706656227	36.0	36.0	36.0	36.0	36.0
16	35.18023434726003	36.0	36.0	36.0	36.0	36.0
17	35.18735945082258	36.0	36.0	36.0	36.0	36.0
18	35.18877973724701	36.0	36.0	36.0	36.0	36.0
19	35.121110372643095	36.0	36.0	36.0	36.0	36.0
20	35.09751040177718	36.0	36.0	36.0	36.0	36.0
21	35.06500405145807	36.0	36.0	36.0	36.0	36.0
22	35.032864153245264	36.0	36.0	36.0	36.0	36.0
23	35.046194360734546	36.0	36.0	36.0	36.0	36.0
24	35.01672387264765	36.0	36.0	36.0	36.0	36.0
25	34.984115553046784	36.0	36.0	36.0	36.0	36.0
26	34.9557385034187	36.0	36.0	36.0	36.0	36.0
27	34.89201589628267	36.0	36.0	36.0	36.0	36.0
28	34.81133861995502	36.0	36.0	36.0	36.0	36.0
29	34.77836475868787	36.0	36.0	36.0	32.0	36.0
30	34.681816692007246	36.0	36.0	36.0	32.0	36.0
31	34.690578766717955	36.0	36.0	36.0	32.0	36.0
32	34.63110017571492	36.0	36.0	36.0	32.0	36.0
33	34.670715241676305	36.0	36.0	36.0	32.0	36.0
34	34.62573404226263	36.0	36.0	36.0	32.0	36.0
35	34.58354152061691	36.0	36.0	36.0	32.0	36.0
36	34.48818931689686	36.0	36.0	36.0	32.0	36.0
37	34.37890965703725	36.0	36.0	36.0	32.0	36.0
38	34.271297468066315	36.0	36.0	36.0	32.0	36.0
39	34.0719711936779	36.0	36.0	36.0	32.0	36.0
40	34.15832916048326	36.0	36.0	36.0	32.0	36.0
41	34.136216393382924	36.0	36.0	36.0	32.0	36.0
42	34.09891748682138	36.0	36.0	36.0	32.0	36.0
43	34.09908682866429	36.0	36.0	36.0	32.0	36.0
44	33.914969909957485	36.0	36.0	36.0	32.0	36.0
45	33.84986161311762	36.0	36.0	36.0	32.0	36.0
46	33.5703201107095	36.0	36.0	36.0	21.0	36.0
47	33.58630060908437	36.0	36.0	36.0	21.0	36.0
48	33.43360252009796	36.0	36.0	36.0	21.0	36.0
49	33.35985323706947	36.0	36.0	36.0	21.0	36.0
50	32.916467128563234	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	3.0
19	4.0
20	14.0
21	53.0
22	179.0
23	569.0
24	1527.0
25	3882.0
26	8449.0
27	16460.0
28	29225.0
29	44379.0
30	63315.0
31	87662.0
32	124172.0
33	194048.0
34	376949.0
35	1245849.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32359555881726	20.323537285138574	13.187470066450205	25.16539708959396
2	9.492787237035396	21.26086443902326	51.85819292394016	17.388155400001185
3	20.866795888805836	26.880132050580546	31.079183915758573	21.173888144855045
4	9.426695922139169	18.251317861922665	41.32947003286688	30.992516183071277
5	8.61162449811994	43.04250844433115	37.210593880022216	11.135273177526699
6	28.41509258745028	41.555236091397504	18.706543441308778	11.32312787984344
7	24.134819839771588	32.722002932528625	22.114598047378408	21.028579180321376
8	20.29135927907652	48.12171501562772	19.174157318715295	12.412768386580467
9	26.67435390968233	13.500625707591297	20.498763379649503	39.32625700307687
10	15.127847601671037	31.007544440180506	35.6459207080384	18.21868725011005
11	34.70136174637121	18.86192163107298	30.76989609138641	15.666820531169398
12	26.503011964552915	22.70784103163826	37.237514333746525	13.551632670062306
13	33.09892366997402	23.19312758453042	26.825081348407902	16.882867397087665
14	17.40171108252327	27.752422000256743	27.874330029343508	26.97153688787648
15	18.967559545487653	43.06868672153835	23.0939070580523	14.8698466749217
16	15.748302122307283	27.49904061204172	39.34009586140748	17.412561404243522
17	15.58723381010042	31.990221874231818	33.45266167138578	18.96988264428198
18	15.557589899859883	27.801833823305817	42.817024639440035	13.823551637394264
19	25.00978723016834	23.024208600016387	28.11334067754946	23.852663492265812
20	23.34588228867453	30.988423702503265	31.61731300615005	14.048381002672153
21	21.039676975882443	25.604259038393256	27.592660032593752	25.76340395313055
22	21.956757662277123	33.942585727187975	28.86587191178184	15.23478469875306
23	18.937788583792464	32.77087328875473	31.54741229988906	16.743925827563743
24	24.42627301542791	27.862785327119866	32.54569399194167	15.165247665510556
25	20.804141845366374	30.967923732941472	33.41295268998708	14.814981731705073
26	13.240014585986074	34.12970510723477	36.93067878098669	15.699601525792476
27	15.758595792220515	31.809507247115764	35.097273146929936	17.33462381373378
28	15.064848746955592	34.71531650467758	29.723305943140694	20.496528805226138
29	18.406031247880335	27.317472218466577	28.616106119516928	25.66039041413616
30	15.481454976523384	30.680480403377135	31.885540462904032	21.952524157195445
31	22.35785737731263	26.98837601561337	31.19323274965298	19.460533857421016
32	17.16422462809481	33.76286383612955	31.667047547597214	17.405863988178428
33	16.450091504220122	33.05614990303284	35.19489033150932	15.298868261237718
34	17.95597250933571	33.02239341039836	34.588443523455695	14.43319055681023
35	15.871993400914475	34.82597279151879	33.54635293089352	15.755680876673217
36	14.328618996413486	32.9507657876263	34.83065809205477	17.889957123905447
37	15.553700219468858	32.35838659205059	29.15157372510262	22.936339463377927
38	18.922172154171555	36.274393758257204	28.158816989792246	16.644617097778987
39	21.964368226856077	27.714709089129506	30.923617260036373	19.39730542397804
40	25.098581034019674	29.619560516600124	31.154065016593297	14.1277934327869
41	22.200047071613042	25.795926803186486	36.36459650204197	15.639429623158504
42	19.237394535272358	35.87548811720822	31.535581001736233	13.351536345783185
43	14.445498545098308	35.81443539253211	34.24108394728121	15.498982115088369
44	14.138533345047804	35.12220303699209	31.244454783123693	19.494808834836412
45	14.704594329332801	35.177046744492216	31.792209069207196	18.326149856967792
46	17.858074442975077	28.380650198136763	30.406675422164458	23.354599936723698
47	23.29683136180199	30.211341180005324	28.93473574954989	17.557091708642798
48	16.787666568337176	30.945064770000037	28.514141237567692	23.753127424095098
49	20.965399009163168	29.774769668903172	30.015627781123662	19.244203540809995
50	17.459408449306483	36.34459127573575	26.320241125214583	19.875759149743182
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	68.0
1	62.0
2	56.0
3	175.5
4	295.0
5	355.5
6	416.0
7	461.5
8	507.0
9	786.0
10	1065.0
11	1633.5
12	2202.0
13	4485.0
14	6768.0
15	9995.5
16	13223.0
17	15943.0
18	18663.0
19	19288.0
20	19913.0
21	20624.0
22	21335.0
23	22491.0
24	23647.0
25	28148.0
26	32649.0
27	57673.5
28	82698.0
29	101248.5
30	119799.0
31	141612.0
32	163425.0
33	206843.0
34	250261.0
35	295938.0
36	341615.0
37	327882.5
38	314150.0
39	263253.0
40	212356.0
41	172148.5
42	131941.0
43	100974.5
44	70008.0
45	61812.0
46	53616.0
47	50258.0
48	46900.0
49	44390.0
50	41880.0
51	40968.5
52	40057.0
53	37835.5
54	35614.0
55	34232.5
56	32851.0
57	30866.5
58	28882.0
59	26249.0
60	23616.0
61	20741.5
62	17867.0
63	15670.0
64	13473.0
65	12398.5
66	11324.0
67	9721.5
68	8119.0
69	7192.5
70	6266.0
71	4848.5
72	3431.0
73	2844.5
74	2258.0
75	1886.5
76	1515.0
77	1225.5
78	936.0
79	734.5
80	533.0
81	397.5
82	262.0
83	197.0
84	132.0
85	90.5
86	49.0
87	32.5
88	16.0
89	23.0
90	30.0
91	19.5
92	9.0
93	9.5
94	10.0
95	15.0
96	20.0
97	16.0
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00946857616285951
2	4.0969800704680575E-4
3	2.731320046978705E-4
4	0.0
5	0.0
6	2.731320046978705E-4
7	4.5522000782978414E-5
8	9.104400156595683E-5
9	0.0012290940211404171
10	0.0010470060180085034
11	9.104400156595683E-5
12	4.5522000782978414E-5
13	7.283520125276546E-4
14	9.104400156595683E-5
15	0.0045522000782978415
16	0.0012290940211404171
17	0.0
18	8.193960140936115E-4
19	0.0
20	4.552200078297841E-4
21	0.0
22	1.8208800313191366E-4
23	9.559620164425467E-4
24	3.186540054808489E-4
25	0.0017298360297531795
26	0.005052942086910604
27	0.006464124111182935
28	0.004096980070468057
29	0.0021395340367999856
30	0.003914892067336143
31	0.007329042126059524
32	0.0035507160610723162
33	0.00546264009395741
34	0.0030499740524595536
35	0.003277584056374446
36	0.008102916139370158
37	0.003323106057157424
38	0.007920828136238244
39	0.004779810082212733
40	0.0031865400548084888
41	0.003960414068119122
42	0.001547748026621266
43	0.0012746160219233955
44	0.0012290940211404171
45	0.0014567040250553092
46	0.0011380500195744604
47	0.0025037100430638126
48	0.0020029680344510505
49	5.007420086127626E-4
50	0.0017753580305361581
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2196740.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.49647782042373
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.89663059666682	18.77821872238572
2	13.559408992634287	7.72790795233314
3	6.288586891687845	5.376077306423703
4	3.5761035704734505	4.076254243181392
5	2.2381916579332803	3.1890289469076563
6	1.5017312475349622	2.5676430712570384
7	1.1313081351578194	2.256680802711281
8	0.8316545686282243	1.8959380775334607
9	0.6273214367738809	1.608880626837292
>10	3.7982442741831433	19.73246956532275
>50	0.30478198338768586	6.053219716630463
>100	0.20754403456850062	11.779066494032655
>500	0.023416337975164143	4.667599970109217
>1k	0.013632799506088712	7.26333144595653
>5k	0.0012830870123377613	2.566389298724837
>10k+	1.6038587654222016E-4	0.46129375965290254
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	10093	0.4594535539026012	No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG	8726	0.3972249788322696	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	8624	0.3925817347524058	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	7948	0.3618088622231124	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	7300	0.33231060571574245	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGA	6701	0.30504292724673837	No Hit
GTGTATATCAATGAGTTACAATGAAAAACATGGAAAATGATAAAAACCAC	5952	0.2709469486602875	No Hit
GTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGATAAAAACCAC	5625	0.2560612544042536	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5276	0.24017407613099412	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	4999	0.22756448191410908	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	4886	0.22242049582563256	No Hit
GTCCTACAGTGTGCATTTCTCATTTTTCACGTTTTTCAGTGATTTCGTCA	4768	0.21704889973324107	No Hit
TTGTAGAACAGTGTATATCAATGAGTTACAATGAGAAACATGGAAAATGA	4456	0.2028460354889518	No Hit
CTGTAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	3767	0.17148137694947968	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	3492	0.15896282673416062	No Hit
CTGTAGGACATGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	3305	0.15045021258774366	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3298	0.15013155858226282	No Hit
GATATACACTGTTCTACAATGCCGGTTTCCAACGTATGTGTTTTTCAGTG	3246	0.14776441454154793	No Hit
TTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGCCATATTTCACGTCCT	3080	0.1402077624115735	No Hit
GTCCTTCAGTGTGCATTTCTCATTTTTCACGTTTTTTAGTGATTTCGTCA	3073	0.13988910840609267	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	3034	0.1381137503755565	No Hit
GTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTGTAACTCACTC	2958	0.13465407831605014	No Hit
GAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGAAACATCCACTTG	2758	0.12554967815945445	No Hit
GTTCTACAGTGTGGTTTTTATCATTTTCCATGTTTTTCATTGTAACTCAT	2413	0.10984458788932691	No Hit
CTGTAGAACATATTAGATGAGTGAGTTACACTGAAAAACACATTCGTTGG	2398	0.10916175787758224	No Hit
TTGATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAG	2366	0.10770505385252693	No Hit
GGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGAAATACACACTT	2310	0.10515582180868013	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	2214	0.10078570973351421	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.5522000782978414E-5	0.0
8	0.0	0.0	0.0	4.5522000782978414E-5	0.0
9	0.0	0.0	0.0	9.104400156595683E-5	0.0
10	0.0	0.0	0.0	9.104400156595683E-5	0.0
11	0.0	0.0	0.0	1.3656600234893525E-4	0.0
12	0.0	0.0	0.0	1.3656600234893525E-4	0.0
13	0.0	0.0	0.0	1.3656600234893525E-4	0.0
14	0.0	0.0	0.0	1.3656600234893525E-4	0.0
15	0.0	0.0	0.0	1.3656600234893525E-4	0.0
16	0.0	0.0	0.0	1.3656600234893525E-4	0.0
17	0.0	0.0	0.0	1.8208800313191366E-4	0.0
18	0.0	0.0	0.0	1.8208800313191366E-4	0.0
19	0.0	0.0	0.0	2.731320046978705E-4	0.0
20	0.0	0.0	0.0	2.731320046978705E-4	0.0
21	0.0	0.0	0.0	3.186540054808489E-4	0.0
22	0.0	0.0	0.0	6.373080109616978E-4	0.0
23	0.0	0.0	0.0	0.001547748026621266	0.0
24	0.0	0.0	0.0	0.003960414068119122	0.0
25	0.0	0.0	0.0	0.005143986088476561	0.0
26	0.0	0.0	0.0	0.006099948104919107	0.0
27	0.0	0.0	0.0	0.006964866119795697	0.0
28	0.0	0.0	0.0	0.009332010160510575	0.0
29	0.0	0.0	0.0	0.01406629824194033	0.0
30	0.0	0.0	0.0	0.027085590465872156	0.0
31	0.0	0.0	0.0	0.057767418993599606	0.0
32	0.0	0.0	0.0	0.09691633966696105	0.0
33	0.0	0.0	0.0	0.12509445815162468	0.0
34	0.0	0.0	0.0	0.1554121106730883	0.0
35	0.0	0.0	0.0	0.19078270528146254	0.0
36	0.0	0.0	0.0	0.23730619008166648	0.0
37	0.0	0.0	0.0	0.32525469559438075	0.0
38	0.0	0.0	0.0	0.42185238125586094	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	5110	0.0	28.801096	4
ACTGTGC	310	0.0	25.54844	8
GTCCTAT	565	0.0	24.52879	1
AGGACCT	8550	0.0	24.52057	5
GGACCTG	8380	0.0	24.256702	6
TGTAGGA	14085	0.0	23.551502	2
CTGTAGG	13565	0.0	23.400795	1
ACTGTTC	6245	0.0	23.356333	8
CTCTAGG	670	0.0	23.31136	1
CCTAGAG	545	0.0	23.007128	3
CTGTGCA	365	0.0	22.904158	9
ATAGGAC	650	0.0	22.674904	3
CTAGGAC	740	0.0	22.592585	3
TAGGACA	3240	0.0	22.338535	4
AGGACCG	345	0.0	22.317871	5
GATATAC	6795	0.0	22.27322	1
GTGTAGG	475	0.0	22.229603	1
TAGAGTG	685	0.0	22.159618	5
ATACACT	6955	0.0	22.01486	4
TCCGTTA	130	6.91216E-11	22.00205	26
>>END_MODULE