FastQCFastQC Report
Thu 2 Feb 2017
SRR4063967_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063967_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140680
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28800.2524809762597749No Hit
TATCAACGCAGAGTACTTTTTTTTT20080.17603534733667636No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16670.14614089841147387No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12480.10940842304590244No Hit
GTCCTAAAGTGTGTATTTCTCATTT12310.10791808394992461No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA1400.014.25125110
CGCCAGT1350.014.07716418
TCGCGTA551.957971E-413.8193959
TCTTATA852.7171154E-713.403532
CGCGTAA500.001498128313.30116710
CGAGCCG1750.013.03200215
GTCGTAG1550.012.87209711
CGCATCG1352.7284841E-1112.66889113
CCGTCGT1650.012.6677799
ACCGTCG1650.012.6677798
TACCGTC1650.012.6666677
TAAGGCC751.4811001E-512.6638894
AGCCGCC2850.012.336040517
ATACCGT1800.012.1388896
GCGTAAC550.003065565612.09197111
CTGACGT550.003072625712.0882574
GGACGTG5700.012.06
GTATTAG1750.011.9370951
AGGACGT5750.011.8940865
TAAGACC802.8712286E-511.8723954
Produced by FastQC (version 0.11.5)