##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063967_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1140680
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.158643090086613	32.0	32.0	32.0	32.0	32.0
2	30.66649980713259	32.0	32.0	32.0	32.0	32.0
3	30.696070764806958	32.0	32.0	32.0	32.0	32.0
4	30.727862327734336	32.0	32.0	32.0	32.0	32.0
5	30.590589823613985	32.0	32.0	32.0	32.0	32.0
6	34.26473068695866	36.0	36.0	36.0	32.0	36.0
7	34.178565417119614	36.0	36.0	36.0	32.0	36.0
8	34.13522109618824	36.0	36.0	36.0	32.0	36.0
9	34.298464950731145	36.0	36.0	36.0	32.0	36.0
10	33.91934284812568	36.0	36.0	36.0	32.0	36.0
11	34.301251884840624	36.0	36.0	36.0	32.0	36.0
12	34.067946312725745	36.0	36.0	36.0	32.0	36.0
13	34.161174036539606	36.0	36.0	36.0	32.0	36.0
14	34.05301925167444	36.0	36.0	36.0	32.0	36.0
15	33.9872111372164	36.0	36.0	36.0	32.0	36.0
16	33.98642038082547	36.0	36.0	36.0	32.0	36.0
17	33.91551004663885	36.0	36.0	36.0	32.0	36.0
18	33.912113826840134	36.0	36.0	36.0	32.0	36.0
19	33.92558210891749	36.0	36.0	36.0	32.0	36.0
20	33.885340323315916	36.0	36.0	36.0	32.0	36.0
21	33.87867412420661	36.0	36.0	36.0	32.0	36.0
22	33.85920328225269	36.0	36.0	36.0	32.0	36.0
23	33.80100641722481	36.0	36.0	36.0	32.0	36.0
24	33.785707648069575	36.0	36.0	36.0	32.0	36.0
25	33.36041571694077	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	15.0
5	9.0
6	42.0
7	21.0
8	50.0
9	61.0
10	101.0
11	21.0
12	64.0
13	59.0
14	99.0
15	202.0
16	365.0
17	448.0
18	599.0
19	854.0
20	1385.0
21	2201.0
22	3342.0
23	5069.0
24	7526.0
25	10712.0
26	15115.0
27	19917.0
28	26583.0
29	35272.0
30	45944.0
31	61217.0
32	84556.0
33	116853.0
34	235739.0
35	466236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.33517149930674	17.232542904425017	11.280428180417394	26.15185741585085
2	15.98200978415248	19.849380161666463	38.51615787904824	25.652452175132822
3	19.027246530295205	24.17032610096444	28.602763840902494	28.199663527837853
4	12.39138687270467	15.700432479916818	36.235381994702934	35.67279865267558
5	14.134086636927307	37.137025502903796	34.01307760289538	14.715810257273517
6	33.566029514012186	35.885012665020085	17.30307406209148	13.245883758876253
7	29.74828335155715	30.79331035407984	21.0130396010694	18.445366693293604
8	27.572982922930002	34.019964904003416	19.540103884851455	18.86694828821513
9	27.613668257086726	14.087935026662922	18.582742772944147	39.7156539433062
10	15.654463625511042	27.166378234338033	32.315531225686826	24.8636269144641
11	36.74097138471659	20.88867448744655	23.274904067536454	19.095450060300408
12	24.988377131809468	23.556702930015422	29.74261268041762	21.712307257757494
13	29.86913989171892	19.9695437527851	25.34210646353145	24.819209891964533
14	23.134427207375534	20.0895625819638	25.72832098667965	31.047689223981017
15	24.59168672416092	28.404306455121503	22.454423291487437	24.549583529230144
16	24.754247056491156	25.837788144558697	25.008377442836014	24.39958735611413
17	23.414176731522083	26.32262682419565	26.03421159046791	24.228984853814353
18	23.917754234760302	25.376803304288625	27.320125770714093	23.38531669023698
19	25.520601675786025	24.86693809803287	25.686408166452175	23.926052059728935
20	25.58116479488482	24.972561529290076	25.693989329922278	23.75228434590283
21	26.024299112974468	24.38192892741936	24.927865024033295	24.665906935572874
22	25.50842171476798	25.287954302799225	25.261108688767216	23.942515293665576
23	24.111138698772855	25.129737559451165	25.93970350964774	24.819420232128238
24	24.67079463726438	25.209629903865892	25.81171821951001	24.307857239359716
25	24.525593808286384	25.15339791515696	25.89665621496237	24.424352061594284
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	105.0
1	105.0
2	321.0
3	537.0
4	537.0
5	537.0
6	1420.0
7	2303.0
8	2303.0
9	2303.0
10	2399.0
11	2495.0
12	2495.0
13	2495.0
14	2831.5
15	3168.0
16	3168.0
17	3168.0
18	5619.5
19	8071.0
20	8071.0
21	8071.0
22	13339.5
23	18608.0
24	18608.0
25	18608.0
26	27676.0
27	36744.0
28	36744.0
29	36744.0
30	47919.5
31	59095.0
32	59095.0
33	59095.0
34	69010.5
35	78926.0
36	78926.0
37	78926.0
38	88243.0
39	97560.0
40	97560.0
41	97560.0
42	109875.5
43	122191.0
44	122191.0
45	122191.0
46	135051.5
47	147912.0
48	147912.0
49	147912.0
50	149801.0
51	151690.0
52	151690.0
53	151690.0
54	140353.0
55	129016.0
56	129016.0
57	129016.0
58	116538.0
59	104060.0
60	104060.0
61	104060.0
62	91213.5
63	78367.0
64	78367.0
65	78367.0
66	64003.0
67	49639.0
68	49639.0
69	49639.0
70	37118.0
71	24597.0
72	24597.0
73	24597.0
74	18553.0
75	12509.0
76	12509.0
77	12509.0
78	9887.5
79	7266.0
80	7266.0
81	7266.0
82	5046.5
83	2827.0
84	2827.0
85	2827.0
86	2154.0
87	1481.0
88	1481.0
89	1481.0
90	1050.5
91	620.0
92	620.0
93	620.0
94	439.5
95	259.0
96	259.0
97	259.0
98	446.5
99	634.0
100	634.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03620647333169688
2	0.005260020338745309
3	0.0018410071185608584
4	0.004295683276642003
5	0.009117368587158538
6	0.012010379773468458
7	0.020251078304169442
8	0.03322579513974121
9	0.04558684293579269
10	0.0533892064382649
11	0.04865518813339412
12	0.06013956587298804
13	0.05996423186169653
14	0.06092856892379984
15	0.05470421152295122
16	0.06259424203106918
17	0.057597222709261146
18	0.07258828067468527
19	0.06987060349966687
20	0.07513062383841218
21	0.07039660553354139
22	0.07267594768033103
23	0.07705929796261879
24	0.0713609425956447
25	0.07311428270855981
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1140680.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.07246176332434
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.79288337853238	43.62038478701507
2	13.303975304968068	14.91973293176067
3	3.992578314015377	6.716210845491155
4	1.6536742533586908	3.7090234536179656
5	0.8616729889641538	2.415806286309096
6	0.5271738170748026	1.7735960220331568
7	0.3334262190145361	1.3087220241607669
8	0.24021761982723427	1.0775674642111503
9	0.16737895884331033	0.8446815242753902
>10	0.9644852281787906	9.890049039104886
>50	0.08656223453325586	3.3695376184710497
>100	0.07030919969097164	7.593238529690415
>500	0.004089571054418144	1.497056639070154
>1k	0.001572911944006978	1.2643928347890732
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2880	0.2524809762597749	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2008	0.17603534733667636	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1667	0.14614089841147387	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1248	0.10940842304590244	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1231	0.10791808394992461	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	8.766700564575516E-5
7	0.0	0.0	0.0	0.0	8.766700564575516E-5
8	0.0	0.0	0.0	0.0	8.766700564575516E-5
9	0.0	0.0	0.0	0.0	8.766700564575516E-5
10	0.0	0.0	0.0	0.0	8.766700564575516E-5
11	0.0	0.0	0.0	0.0	8.766700564575516E-5
12	0.0	0.0	0.0	0.0	3.5066802258302063E-4
13	0.0	0.0	0.0	0.0	3.5066802258302063E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	140	0.0	14.251251	10
CGCCAGT	135	0.0	14.077164	18
TCGCGTA	55	1.957971E-4	13.819395	9
TCTTATA	85	2.7171154E-7	13.40353	2
CGCGTAA	50	0.0014981283	13.301167	10
CGAGCCG	175	0.0	13.032002	15
GTCGTAG	155	0.0	12.872097	11
CGCATCG	135	2.7284841E-11	12.668891	13
CCGTCGT	165	0.0	12.667779	9
ACCGTCG	165	0.0	12.667779	8
TACCGTC	165	0.0	12.666667	7
TAAGGCC	75	1.4811001E-5	12.663889	4
AGCCGCC	285	0.0	12.3360405	17
ATACCGT	180	0.0	12.138889	6
GCGTAAC	55	0.0030655656	12.091971	11
CTGACGT	55	0.0030726257	12.088257	4
GGACGTG	570	0.0	12.0	6
GTATTAG	175	0.0	11.937095	1
AGGACGT	575	0.0	11.894086	5
TAAGACC	80	2.8712286E-5	11.872395	4
>>END_MODULE