##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063967_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1140680
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.310689237998385	32.0	32.0	32.0	32.0	32.0
2	31.37933338008907	32.0	32.0	32.0	32.0	32.0
3	31.462554791878528	32.0	32.0	32.0	32.0	32.0
4	31.549673002068943	32.0	32.0	32.0	32.0	32.0
5	31.493730055756217	32.0	32.0	32.0	32.0	32.0
6	35.076614826244	36.0	36.0	36.0	36.0	36.0
7	35.095219518182134	36.0	36.0	36.0	36.0	36.0
8	35.04178735491111	36.0	36.0	36.0	36.0	36.0
9	35.16181663569099	36.0	36.0	36.0	36.0	36.0
10	35.00552039134551	36.0	36.0	36.0	36.0	36.0
11	35.15053827541467	36.0	36.0	36.0	36.0	36.0
12	35.06107672616334	36.0	36.0	36.0	36.0	36.0
13	35.107197461163516	36.0	36.0	36.0	36.0	36.0
14	35.05362327734334	36.0	36.0	36.0	36.0	36.0
15	35.01140460076446	36.0	36.0	36.0	36.0	36.0
16	35.03509748571028	36.0	36.0	36.0	36.0	36.0
17	34.998613107970684	36.0	36.0	36.0	36.0	36.0
18	35.003646947434866	36.0	36.0	36.0	36.0	36.0
19	34.99538608549286	36.0	36.0	36.0	36.0	36.0
20	34.98343093593295	36.0	36.0	36.0	36.0	36.0
21	34.97786934109479	36.0	36.0	36.0	36.0	36.0
22	34.96925079776975	36.0	36.0	36.0	36.0	36.0
23	34.91860118525791	36.0	36.0	36.0	32.0	36.0
24	34.89567187993127	36.0	36.0	36.0	32.0	36.0
25	34.86374180313497	36.0	36.0	36.0	32.0	36.0
26	34.80701599046183	36.0	36.0	36.0	32.0	36.0
27	34.79705000526002	36.0	36.0	36.0	32.0	36.0
28	34.774437177823756	36.0	36.0	36.0	32.0	36.0
29	34.740510923308904	36.0	36.0	36.0	32.0	36.0
30	34.72054476277308	36.0	36.0	36.0	32.0	36.0
31	34.70799172423467	36.0	36.0	36.0	32.0	36.0
32	34.66981800329628	36.0	36.0	36.0	32.0	36.0
33	34.65067152926325	36.0	36.0	36.0	32.0	36.0
34	34.629908475646104	36.0	36.0	36.0	32.0	36.0
35	34.59095802503769	36.0	36.0	36.0	32.0	36.0
36	34.56376021320616	36.0	36.0	36.0	32.0	36.0
37	34.54631623242277	36.0	36.0	36.0	32.0	36.0
38	34.50949609005155	36.0	36.0	36.0	32.0	36.0
39	34.478474243433745	36.0	36.0	36.0	32.0	36.0
40	34.47773608724621	36.0	36.0	36.0	32.0	36.0
41	34.44756636392327	36.0	36.0	36.0	32.0	36.0
42	34.40029806781919	36.0	36.0	36.0	32.0	36.0
43	34.38253059578497	36.0	36.0	36.0	32.0	36.0
44	34.31060946102325	36.0	36.0	36.0	32.0	36.0
45	34.282009853771434	36.0	36.0	36.0	32.0	36.0
46	34.239857804116845	36.0	36.0	36.0	32.0	36.0
47	34.2067749061963	36.0	36.0	36.0	32.0	36.0
48	34.1506241890802	36.0	36.0	36.0	32.0	36.0
49	34.12974804502577	36.0	36.0	36.0	32.0	36.0
50	33.535323666584844	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	0.0
20	5.0
21	32.0
22	102.0
23	333.0
24	865.0
25	2010.0
26	4316.0
27	7761.0
28	13223.0
29	20447.0
30	29429.0
31	42009.0
32	61575.0
33	98026.0
34	201075.0
35	659470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.56125398588622	18.03518714486488	11.819089644191983	26.58446922505692
2	15.321906171263656	20.5647713544799	38.07538350133342	26.037938972923026
3	18.13230213285619	24.716900577464084	29.061513469632505	28.089283820047218
4	12.132762913349932	16.48779675281411	36.390048041519094	34.989392292316865
5	13.696917628081495	37.43679208892941	33.9230108356419	14.943279447347196
6	33.51027457306168	36.17465020864748	17.150471648490374	13.16460356980047
7	29.661167023179157	30.808991128099027	21.061033769330574	18.46880807939124
8	27.53403893645802	34.078912647642326	19.442279554546023	18.944768861353634
9	27.59846230050015	13.85516343536446	18.412592654285	40.133781609850395
10	15.533570329588443	27.22990536222291	32.39855698211131	24.837967326077333
11	36.89544832906687	20.825823193183012	23.28234035838272	18.996388119367396
12	24.82591448046113	23.552370307055035	29.81164661275122	21.810068599732617
13	30.007609536116362	19.933653261142098	25.289171139168598	24.769566063572938
14	23.072546454909194	19.9587788293959	25.727200361890667	31.241474353804232
15	24.53151424240538	28.35856617714745	22.449969008708244	24.65995057173892
16	24.779472487318902	25.90222973435004	25.00390120227164	24.31439657605942
17	23.3767577234632	26.290545990111163	26.072693481081462	24.26000280534418
18	24.05505843449384	25.238301047007965	27.420422397536903	23.286218120961287
19	25.585177262685416	24.79056352351229	25.808815794087735	23.81544341971456
20	25.551166330023005	24.827119452392978	25.78287185097907	23.838842366604947
21	26.02228495283515	24.391766314829752	24.975365571413544	24.610583160921557
22	25.493522273595175	25.124005196909206	25.42075853132961	23.961713998166005
23	24.041024975650295	24.97801732489035	26.05448898060878	24.926468718850572
24	24.688868705925255	25.137024010323707	25.89885296087583	24.275254322875206
25	24.632844142864656	25.026738905545916	25.9703154314169	24.370101520172533
26	23.904438356164384	25.85871780821918	26.410695890410956	23.826147945205477
27	24.52880641479063	25.53501885826406	25.601123605356413	24.3350511215889
28	23.893224200948072	25.53240174534666	25.924727760813514	24.649646292891756
29	24.277165260448445	25.128260427406175	25.67461653375976	24.91995777838562
30	23.85929967202537	25.487512679318662	26.199220260258084	24.45396738839789
31	24.95423445286499	25.010520815433335	25.43468502432062	24.600559707381052
32	24.252724121394685	25.652285596808117	25.361835385830624	24.73315489596657
33	23.961291329387535	25.31800603705168	26.06873612052361	24.651966513037173
34	24.565508607418266	25.36146247207718	26.223168666121943	23.849860254382612
35	24.760881953272257	25.340463770657077	25.979485381142332	23.91916889492833
36	23.954978002766968	25.752105474496712	25.82268248760738	24.47023403512894
37	24.631934246575344	25.49356712328767	25.409753424657534	24.464745205479453
38	24.33117478620564	25.911683684294513	25.563791117166502	24.193350412333352
39	25.066169398921478	25.09606507965314	25.370035760845074	24.46772976058031
40	25.49815676080848	25.219194234891358	25.643511841880322	23.63913716241984
41	24.405793594643	25.298012780555464	26.30060869389877	23.995584930902766
42	25.008613411673736	25.947541035345235	25.618434191343102	23.425411361637924
43	24.160186557023504	25.49790912358526	25.995774413283424	24.346129906107812
44	23.890691929101067	25.918454657191504	25.463281977371118	24.727571436336316
45	24.0649095045434	26.004567511057147	25.415262149710916	24.515260834688537
46	24.254057480202444	25.248472171204494	25.491925366735895	25.00554498185717
47	24.736314709304008	25.33815978307277	25.644736945923363	24.280788561699858
48	24.84265729667746	25.87688037571132	24.742364747519645	24.538097580091577
49	24.520021460958237	25.82126738881155	25.07285153715867	24.58585961307155
50	24.290370713249978	26.35539237603295	24.88747801722853	24.466758893488542
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10.0
1	15.5
2	21.0
3	61.0
4	101.0
5	137.0
6	173.0
7	173.5
8	174.0
9	248.5
10	323.0
11	494.0
12	665.0
13	1221.5
14	1778.0
15	2452.0
16	3126.0
17	3473.0
18	3820.0
19	3934.0
20	4048.0
21	4401.5
22	4755.0
23	5415.0
24	6075.0
25	6991.0
26	7907.0
27	10210.5
28	12514.0
29	14842.5
30	17171.0
31	20367.5
32	23564.0
33	28288.0
34	33012.0
35	38303.5
36	43595.0
37	47125.0
38	50655.0
39	52415.0
40	54175.0
41	56825.5
42	59476.0
43	60327.5
44	61179.0
45	66078.5
46	70978.0
47	75263.5
48	79549.0
49	81880.5
50	84212.0
51	82667.0
52	81122.0
53	79824.5
54	78527.0
55	78566.5
56	78606.0
57	75910.5
58	73215.0
59	66360.5
60	59506.0
61	51640.5
62	43775.0
63	37990.5
64	32206.0
65	27395.5
66	22585.0
67	19186.0
68	15787.0
69	13973.0
70	12159.0
71	9580.0
72	7001.0
73	5987.0
74	4973.0
75	3992.0
76	3011.0
77	2565.0
78	2119.0
79	1726.0
80	1333.0
81	1059.5
82	786.0
83	610.0
84	434.0
85	323.0
86	212.0
87	156.5
88	101.0
89	75.0
90	49.0
91	45.0
92	41.0
93	34.0
94	27.0
95	26.5
96	26.0
97	19.0
98	12.0
99	11.5
100	11.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00815303152505523
2	5.26002033874531E-4
3	2.630010169372655E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	8.766700564575517E-5
9	0.0013150050846863273
10	0.0013150050846863273
11	0.0
12	4.3833502822877585E-4
13	5.26002033874531E-4
14	3.506680225830207E-4
15	0.0032436792088929413
16	5.26002033874531E-4
17	0.0
18	8.766700564575517E-5
19	0.0
20	7.013360451660414E-4
21	0.0
22	1.7533401129151034E-4
23	7.890030508117964E-4
24	3.506680225830207E-4
25	0.0017533401129151034
26	0.004821685310516534
27	0.00578602237261984
28	0.004120349265350492
29	0.0028053441806641655
30	0.0037696812427674723
31	0.0073640284742434335
32	0.0032436792088929413
33	0.004997019321808044
34	0.0031560122032471857
35	0.0026300101693726547
36	0.007188694462951923
37	0.004821685310516534
38	0.008591366553284007
39	0.004120349265350492
40	0.002191675141143879
41	0.0035943472314759616
42	0.001490339095977838
43	8.766700564575517E-4
44	0.0012273380790405722
45	0.0013150050846863273
46	7.890030508117964E-4
47	0.0018410071185608584
48	0.001490339095977838
49	3.506680225830207E-4
50	0.0012273380790405722
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1140680.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.017768795495115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.85745108460841	47.328482669980936
2	12.894455243425346	15.477928670875244
3	3.786181209383621	6.817144453279028
4	1.5538169273859017	3.7302650039349476
5	0.7873675853763921	2.362802284809378
6	0.47325486573701137	1.7042220667888224
7	0.3046384662035015	1.2798604721571205
8	0.21554521798914675	1.0349234446597764
9	0.16939750450135863	0.9150174233726749
>10	0.829650399998638	8.986233006675011
>50	0.07172858753708387	3.0460682008567486
>100	0.05327519227365069	6.066246799767053
>500	0.0032377155798691804	1.2508055028432075
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.766700564575516E-5
2	0.0	0.0	0.0	0.0	8.766700564575516E-5
3	0.0	0.0	0.0	0.0	8.766700564575516E-5
4	0.0	0.0	0.0	0.0	8.766700564575516E-5
5	0.0	0.0	0.0	0.0	8.766700564575516E-5
6	0.0	0.0	0.0	0.0	1.7533401129151031E-4
7	0.0	0.0	0.0	0.0	1.7533401129151031E-4
8	0.0	0.0	0.0	0.0	1.7533401129151031E-4
9	0.0	0.0	0.0	0.0	1.7533401129151031E-4
10	0.0	0.0	0.0	0.0	1.7533401129151031E-4
11	0.0	0.0	0.0	0.0	1.7533401129151031E-4
12	0.0	0.0	0.0	0.0	5.26002033874531E-4
13	0.0	0.0	0.0	0.0	6.136690395202862E-4
14	0.0	0.0	0.0	0.0	6.136690395202862E-4
15	0.0	0.0	0.0	0.0	7.013360451660413E-4
16	0.0	0.0	0.0	0.0	7.890030508117964E-4
17	0.0	0.0	0.0	0.0	7.890030508117964E-4
18	0.0	0.0	0.0	8.766700564575516E-5	7.890030508117964E-4
19	0.0	0.0	0.0	8.766700564575516E-5	7.890030508117964E-4
20	0.0	0.0	0.0	8.766700564575516E-5	9.643370621033068E-4
21	0.0	0.0	0.0	8.766700564575516E-5	9.643370621033068E-4
22	0.0	0.0	0.0	8.766700564575516E-5	9.643370621033068E-4
23	0.0	0.0	0.0	2.630010169372655E-4	9.643370621033068E-4
24	0.0	0.0	0.0	0.001052004067749062	9.643370621033068E-4
25	0.0	0.0	0.0	0.0013150050846863276	9.643370621033068E-4
26	0.0	0.0	0.0	0.0014903390959778377	9.643370621033068E-4
27	0.0	0.0	0.0	0.0019286741242066135	9.643370621033068E-4
28	0.0	0.0	0.0	0.0029806781919556754	9.643370621033068E-4
29	0.0	0.0	0.0	0.0053476873443910646	9.643370621033068E-4
30	0.0	0.0	0.0	0.009205035592804292	9.643370621033068E-4
31	0.0	0.0	0.0	0.017796402146088297	9.643370621033068E-4
32	0.0	0.0	0.0	0.028316442823578918	9.643370621033068E-4
33	0.0	0.0	0.0	0.039274818529298317	9.643370621033068E-4
34	0.0	0.0	0.0	0.05277553739874461	0.001052004067749062
35	0.0	0.0	0.0	0.06759126135287723	0.001052004067749062
36	0.0	0.0	0.0	0.08915734474173301	0.001052004067749062
37	0.0	0.0	0.0	0.12168180383630817	0.001052004067749062
38	0.0	0.0	0.0	0.16551530665918576	0.001052004067749062
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCTAC	745	0.0	18.89901	1
TCCTACA	845	0.0	18.484085	2
GTATCAA	2240	0.0	18.463972	1
ATAAGAC	145	6.313712E-9	18.20499	3
ATAGTAG	135	5.531365E-8	17.924051	3
GTCCTAA	480	0.0	17.416372	1
GTGTATA	455	0.0	17.4063	1
TAATACT	215	0.0	17.39429	4
TAAGACT	165	1.6607373E-9	17.332281	4
GATATAC	395	0.0	16.708578	1
TAGGACG	725	0.0	16.68864	4
TTAGGAC	490	0.0	16.610508	3
GTTATAG	240	0.0	16.499722	1
AACGCAG	2470	0.0	16.47673	6
AGGACGT	760	0.0	16.209541	5
GTATATC	395	0.0	16.15021	3
GTATATA	165	3.11029E-8	15.999729	1
GGACGTG	770	0.0	15.999028	6
GGCGTTA	220	1.8189894E-11	15.998325	42
AATCACG	360	0.0	15.890013	34
>>END_MODULE