FastQCFastQC Report
Thu 2 Feb 2017
SRR4063966_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063966_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657561
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT41680.2514537926507682No Hit
TATCAACGCAGAGTACTTTTTTTTT28500.1719393735735819No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17770.10720570766324737No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG16790.10129340639650668No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACA250.00604337418.9939352
GGTCCAA3350.012.19492111
GACGTGG1501.4551915E-1112.0334857
GCGCGCT802.8653696E-511.87550915
CAAGACG2900.011.7914744
AATAGCG2450.011.6313975
CGCAAGA2900.011.4618582
CGCCGGT3150.011.4604647
TCCAACG1001.9272447E-611.40117718
AAGACGG3000.011.3987685
ATAGTCC752.0783838E-411.3973923
ACGGTAT2200.011.2284339
GCCGTTA1104.9655864E-711.22775515
CCGTCGT2550.011.1776249
CGGAATT855.3207936E-511.1769515
ATAGCGT2400.011.0828066
TTAGATA600.005881983311.0818014
GTATCAA27350.010.9763741
AGCGTAT2450.010.8579368
GGTTCTA3500.010.85760813
Produced by FastQC (version 0.11.5)