##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063966_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1657561
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26286393079953	32.0	32.0	32.0	32.0	32.0
2	30.863442129731574	32.0	32.0	32.0	32.0	32.0
3	30.88826353901908	32.0	32.0	32.0	32.0	32.0
4	30.915320160163034	32.0	32.0	32.0	32.0	32.0
5	30.82457779834347	32.0	32.0	32.0	32.0	32.0
6	34.48276835663967	36.0	36.0	36.0	32.0	36.0
7	34.42095464359984	36.0	36.0	36.0	32.0	36.0
8	34.38221640108569	36.0	36.0	36.0	32.0	36.0
9	34.51608115779751	36.0	36.0	36.0	32.0	36.0
10	34.21370736883892	36.0	36.0	36.0	32.0	36.0
11	34.496129554206455	36.0	36.0	36.0	32.0	36.0
12	34.31330129026926	36.0	36.0	36.0	32.0	36.0
13	34.391642901829854	36.0	36.0	36.0	32.0	36.0
14	34.291403453628554	36.0	36.0	36.0	32.0	36.0
15	34.23572888116938	36.0	36.0	36.0	32.0	36.0
16	34.22828119146143	36.0	36.0	36.0	32.0	36.0
17	34.148962240303675	36.0	36.0	36.0	32.0	36.0
18	34.15030517730569	36.0	36.0	36.0	32.0	36.0
19	34.16588047136727	36.0	36.0	36.0	32.0	36.0
20	34.13519140472055	36.0	36.0	36.0	32.0	36.0
21	34.12310316181426	36.0	36.0	36.0	32.0	36.0
22	34.10439374478526	36.0	36.0	36.0	32.0	36.0
23	34.04433924302032	36.0	36.0	36.0	32.0	36.0
24	34.034090449763234	36.0	36.0	36.0	32.0	36.0
25	33.63618774814321	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	5.0
4	16.0
5	24.0
6	65.0
7	28.0
8	99.0
9	114.0
10	142.0
11	49.0
12	86.0
13	84.0
14	122.0
15	257.0
16	441.0
17	580.0
18	819.0
19	1099.0
20	1583.0
21	2311.0
22	3599.0
23	5614.0
24	8555.0
25	12585.0
26	17828.0
27	23844.0
28	32916.0
29	44270.0
30	58893.0
31	79924.0
32	113053.0
33	160754.0
34	343624.0
35	744178.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.28409382383739	17.134709569174408	11.511073156158812	26.070123450829392
2	16.585056833264954	19.733933441127494	37.65505466129305	26.0259550643145
3	18.53390727797603	23.951832088010207	28.736105119952214	28.77815551406154
4	12.5488615610722	15.635277217526028	36.08790163916623	35.727959582235535
5	14.61238595904813	36.671696605967504	33.51061807918263	15.205299355801735
6	33.7492864978236	35.74190348502822	17.144265614685896	13.364544402462291
7	30.337566822734725	30.431702473414223	20.8547275708235	18.376003133027556
8	28.264896041985143	32.56950802279104	19.507811561726935	19.657784373496877
9	27.83001813775641	13.926658276822604	18.420355331021597	39.822968254399385
10	15.873702865993856	26.464461350220596	31.66826931492974	25.993566468855807
11	36.940061111131236	20.949938852651186	22.908344168426236	19.20165586779134
12	24.91299452763499	23.528875554254288	28.92505606683952	22.633073851271202
13	29.509837538152457	19.48775499362516	25.287350770776186	25.715056697446197
14	23.748613763180945	19.61951459223659	25.350460589823015	31.28141105475945
15	25.122871999430156	27.30716249286189	22.260084196445497	25.30988131126245
16	25.71525507222579	25.61148030271565	23.89970974456556	24.773554880493
17	24.073012070884754	25.674957803818543	25.27659928960706	24.97543083568964
18	24.6399203042927	24.9109460846465	26.364004105536438	24.08512950552436
19	25.687184391459645	24.662566019691802	25.429988335615494	24.220261253233062
20	25.96184092395173	24.105629674263422	25.014595988472944	24.917933413311903
21	26.989609558481703	23.970223325062033	24.411560498209898	24.628606618246366
22	25.988081797269803	24.230177082515745	24.938265641886385	24.84347547832807
23	24.550884826177498	24.116638016762458	25.413521667961795	25.918955489098245
24	24.775384251267756	24.849285111814297	25.323361547281152	25.051969089636795
25	24.961736056981426	24.36623740610617	25.410880786157236	25.261145750755166
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	254.0
1	254.0
2	451.0
3	648.0
4	648.0
5	648.0
6	1621.0
7	2594.0
8	2594.0
9	2594.0
10	2744.5
11	2895.0
12	2895.0
13	2895.0
14	3560.0
15	4225.0
16	4225.0
17	4225.0
18	7369.5
19	10514.0
20	10514.0
21	10514.0
22	17179.5
23	23845.0
24	23845.0
25	23845.0
26	35483.0
27	47121.0
28	47121.0
29	47121.0
30	59777.5
31	72434.0
32	72434.0
33	72434.0
34	88620.0
35	104806.0
36	104806.0
37	104806.0
38	120128.5
39	135451.0
40	135451.0
41	135451.0
42	155375.5
43	175300.0
44	175300.0
45	175300.0
46	194706.5
47	214113.0
48	214113.0
49	214113.0
50	219400.5
51	224688.0
52	224688.0
53	224688.0
54	209064.5
55	193441.0
56	193441.0
57	193441.0
58	176763.5
59	160086.0
60	160086.0
61	160086.0
62	140919.5
63	121753.0
64	121753.0
65	121753.0
66	100264.5
67	78776.0
68	78776.0
69	78776.0
70	59551.5
71	40327.0
72	40327.0
73	40327.0
74	31190.5
75	22054.0
76	22054.0
77	22054.0
78	17336.0
79	12618.0
80	12618.0
81	12618.0
82	8659.5
83	4701.0
84	4701.0
85	4701.0
86	3566.0
87	2431.0
88	2431.0
89	2431.0
90	1783.0
91	1135.0
92	1135.0
93	1135.0
94	795.0
95	455.0
96	455.0
97	455.0
98	675.5
99	896.0
100	896.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03776633258142536
2	0.004886697985775486
3	0.0015685697238291682
4	0.004343731542911543
5	0.009170099923924369
6	0.014660093957326458
7	0.022804590600285602
8	0.03583578522902023
9	0.04802236539107761
10	0.05598587321974877
11	0.054718951519732904
12	0.06370806262936929
13	0.06328575539603068
14	0.06624190602940103
15	0.05785609096739125
16	0.06569893958653708
17	0.0611138896245749
18	0.07607563160571466
19	0.07462772109141082
20	0.07746321251525586
21	0.07426574346283485
22	0.07667892765334126
23	0.08204826247721804
24	0.07770453093430649
25	0.07854914540098373
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1657561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.16493217845163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.35657193395116	36.557358161548684
2	14.558735448659121	14.315584818746906
3	4.680581505702568	6.9036141685074535
4	2.083441985305846	4.097291356212022
5	1.1180570107273338	2.7484598552025865
6	0.6809219360865223	2.0086488483908305
7	0.44688622243820014	1.5379791572360992
8	0.3056423411678873	1.202150797950588
9	0.23922752686558438	1.0585444620208653
>10	1.3350335371853326	11.960767822607508
>50	0.1004023236990015	3.391000171355486
>100	0.08082270869585642	8.318403995660614
>500	0.009979433160095223	3.2616534203849046
>1k	0.003696086355590823	2.6385429641756537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4168	0.2514537926507682	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2850	0.1719393735735819	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1777	0.10720570766324737	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1679	0.10129340639650668	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8098881428798097E-4
2	0.0	6.032960476266032E-5	0.0	0.0	1.8098881428798097E-4
3	0.0	6.032960476266032E-5	0.0	0.0	1.8098881428798097E-4
4	0.0	6.032960476266032E-5	0.0	0.0	1.8098881428798097E-4
5	0.0	6.032960476266032E-5	0.0	0.0	2.4131841905064127E-4
6	0.0	6.032960476266032E-5	0.0	0.0	3.016480238133016E-4
7	0.0	6.032960476266032E-5	0.0	6.032960476266032E-5	3.016480238133016E-4
8	0.0	6.032960476266032E-5	0.0	6.032960476266032E-5	3.016480238133016E-4
9	0.0	6.032960476266032E-5	0.0	1.8098881428798097E-4	3.016480238133016E-4
10	0.0	6.032960476266032E-5	0.0	1.8098881428798097E-4	3.016480238133016E-4
11	0.0	6.032960476266032E-5	0.0	1.8098881428798097E-4	3.016480238133016E-4
12	0.0	6.032960476266032E-5	0.0	1.8098881428798097E-4	4.8263683810128254E-4
13	0.0	6.032960476266032E-5	0.0	1.8098881428798097E-4	5.429664428639429E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACA	25	0.006043374	18.993935	2
GGTCCAA	335	0.0	12.194921	11
GACGTGG	150	1.4551915E-11	12.033485	7
GCGCGCT	80	2.8653696E-5	11.875509	15
CAAGACG	290	0.0	11.791474	4
AATAGCG	245	0.0	11.631397	5
CGCAAGA	290	0.0	11.461858	2
CGCCGGT	315	0.0	11.460464	7
TCCAACG	100	1.9272447E-6	11.401177	18
AAGACGG	300	0.0	11.398768	5
ATAGTCC	75	2.0783838E-4	11.397392	3
ACGGTAT	220	0.0	11.228433	9
GCCGTTA	110	4.9655864E-7	11.227755	15
CCGTCGT	255	0.0	11.177624	9
CGGAATT	85	5.3207936E-5	11.17695	15
ATAGCGT	240	0.0	11.082806	6
TTAGATA	60	0.0058819833	11.081801	4
GTATCAA	2735	0.0	10.976374	1
AGCGTAT	245	0.0	10.857936	8
GGTTCTA	350	0.0	10.857608	13
>>END_MODULE