FastQCFastQC Report
Thu 2 Feb 2017
SRR4063966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1657561
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA17360.10473219386797832No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA16670.10056945113935474No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA33350.021.5717181
TAGGACG4850.020.8645784
GTATAAT1851.8189894E-1219.027471
ATGCGTA1102.7978804E-617.99987622
CGTCGTA4050.017.92526210
AACGCAG41050.017.3094356
CCGTCGT3950.017.2651849
CTATTCC5150.017.086234
TCGATCG1307.3236697E-716.9224541
GGTATCA17250.016.8351761
ATACCGT4200.016.7607786
AATCGTA1451.2505734E-716.68903713
CGAGCCG6500.016.58400215
GTATTAC1602.1300366E-816.5003851
TGCGTAT1206.4098385E-616.49988623
TACCGTC4150.016.432637
AGGACGT5900.016.4056785
GGACGTG5900.016.4056786
TACGACG4050.016.2952025
TATGGCG5050.016.1177316
Produced by FastQC (version 0.11.5)