##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063966_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1657561
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332579012175117	32.0	32.0	32.0	32.0	32.0
2	31.416922212817507	32.0	32.0	32.0	32.0	32.0
3	31.489081849778078	32.0	32.0	32.0	32.0	32.0
4	31.572249226423644	32.0	32.0	32.0	32.0	32.0
5	31.522709571472785	32.0	32.0	32.0	32.0	32.0
6	35.111627264396304	36.0	36.0	36.0	36.0	36.0
7	35.13012914758492	36.0	36.0	36.0	36.0	36.0
8	35.07891655269399	36.0	36.0	36.0	36.0	36.0
9	35.188536651139835	36.0	36.0	36.0	36.0	36.0
10	35.0437576656304	36.0	36.0	36.0	36.0	36.0
11	35.18550508850051	36.0	36.0	36.0	36.0	36.0
12	35.09219208222201	36.0	36.0	36.0	36.0	36.0
13	35.13442039237168	36.0	36.0	36.0	36.0	36.0
14	35.0845688333642	36.0	36.0	36.0	36.0	36.0
15	35.05256518462971	36.0	36.0	36.0	36.0	36.0
16	35.068343789459334	36.0	36.0	36.0	36.0	36.0
17	35.02554174476837	36.0	36.0	36.0	36.0	36.0
18	35.02720563526772	36.0	36.0	36.0	36.0	36.0
19	35.024232592345015	36.0	36.0	36.0	36.0	36.0
20	35.015773778461245	36.0	36.0	36.0	36.0	36.0
21	35.009901898029696	36.0	36.0	36.0	36.0	36.0
22	34.990016053707826	36.0	36.0	36.0	36.0	36.0
23	34.93938986257519	36.0	36.0	36.0	36.0	36.0
24	34.912964289097054	36.0	36.0	36.0	32.0	36.0
25	34.88324049612654	36.0	36.0	36.0	32.0	36.0
26	34.83145175351013	36.0	36.0	36.0	32.0	36.0
27	34.826151797731725	36.0	36.0	36.0	32.0	36.0
28	34.8022787698311	36.0	36.0	36.0	32.0	36.0
29	34.779297413488855	36.0	36.0	36.0	32.0	36.0
30	34.75407662221782	36.0	36.0	36.0	32.0	36.0
31	34.74676829389688	36.0	36.0	36.0	32.0	36.0
32	34.7127128353044	36.0	36.0	36.0	32.0	36.0
33	34.68705827417513	36.0	36.0	36.0	32.0	36.0
34	34.67519204421436	36.0	36.0	36.0	32.0	36.0
35	34.63846157094671	36.0	36.0	36.0	32.0	36.0
36	34.607243413666225	36.0	36.0	36.0	32.0	36.0
37	34.597545429700624	36.0	36.0	36.0	32.0	36.0
38	34.56194734311437	36.0	36.0	36.0	32.0	36.0
39	34.558051860534846	36.0	36.0	36.0	32.0	36.0
40	34.53955299382647	36.0	36.0	36.0	32.0	36.0
41	34.51578674932627	36.0	36.0	36.0	32.0	36.0
42	34.45878914863465	36.0	36.0	36.0	32.0	36.0
43	34.434303775245674	36.0	36.0	36.0	32.0	36.0
44	34.389807675252975	36.0	36.0	36.0	32.0	36.0
45	34.35117199306692	36.0	36.0	36.0	32.0	36.0
46	34.31526622549638	36.0	36.0	36.0	32.0	36.0
47	34.28809558139942	36.0	36.0	36.0	32.0	36.0
48	34.243334031145764	36.0	36.0	36.0	32.0	36.0
49	34.22092459945667	36.0	36.0	36.0	32.0	36.0
50	33.61806956124089	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	7.0
20	18.0
21	49.0
22	183.0
23	520.0
24	1371.0
25	2948.0
26	6041.0
27	11042.0
28	18471.0
29	28400.0
30	40543.0
31	57917.0
32	84321.0
33	137160.0
34	286715.0
35	981852.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46331880469357	18.042482997630046	12.023897440352934	26.47030075732345
2	15.844742219404145	20.555481483849988	37.185899938041196	26.41387635870467
3	17.818512425213733	24.532550011854795	29.03399400442941	28.61494355850206
4	12.176082810828682	16.27143737093235	36.17990529458645	35.372574523652524
5	14.201890609154052	37.09619133172173	33.37494065075132	15.326977408372905
6	33.78940586091105	36.06703355176712	16.894612435884596	13.248948151437235
7	30.163173482001564	30.69407400391298	20.877663024166228	18.265089489919227
8	28.23497898418218	32.815262907368115	19.336181292875494	19.613576815574206
9	27.738509505630017	13.858395312582955	18.321745908404242	40.08134927338279
10	15.75199285209668	26.65357102565294	31.757975179014313	25.836460943236066
11	37.164446535872	20.96123217259104	22.728468351070248	19.14585294046671
12	24.776040884265214	23.671644474368	28.88678941357673	22.665525227790052
13	29.52916682503283	19.543531390587678	25.366278322657948	25.56102346172154
14	23.74419251441427	19.54506596748592	25.3344828147897	31.376258703310107
15	25.167105584665922	27.337441967800615	22.2383912976588	25.25706114987466
16	25.74566243130687	25.66771620575632	23.83736749292481	24.749253870012
17	24.10927863288289	25.77847813745618	25.17011440302951	24.942128826631418
18	24.699089924617887	24.914648382708265	26.318101058486747	24.068160634187098
19	25.681287144183536	24.618460497079745	25.427239178527973	24.273013180208753
20	26.091956033985035	24.034706582534614	25.04022495811597	24.83311242536438
21	27.02392249817654	24.04864738009642	24.383114708900607	24.544315412826435
22	26.004052952628477	24.23476236412986	25.046438825331496	24.714745857910163
23	24.528234727015942	24.087886285248537	25.411827449049834	25.972051538685687
24	24.8024952414852	24.76309986697274	25.47565540811617	24.95874948342589
25	24.99665162815335	24.221458297386096	25.561108691166694	25.220781383293854
26	24.62576932150082	25.008039375746243	25.717435098255347	24.648756204497584
27	25.29289180047761	24.62788955001635	24.928473945933423	25.150744703572624
28	24.574444850683143	24.598095182934113	25.760400414604806	25.067059551777938
29	24.682702337779553	24.636367859558266	25.44770387924559	25.23322592341659
30	24.589606109950388	24.89446410935069	25.728555113053268	24.787374667645658
31	25.318205403579057	24.500187037685077	24.836794536086206	25.34481302264966
32	24.99862745015231	24.8125036576191	24.779441797551613	25.40942709467698
33	24.603390554496055	24.461549321352145	25.27899163010027	25.65606849405154
34	25.165489397336245	24.62859907765151	25.403979950624674	24.801931574387577
35	25.730990623893295	24.355856461887733	25.358926728088075	24.554226186130894
36	24.72873502190151	24.989682759952213	25.080788213005757	25.20079400514052
37	25.660365975058674	24.748806930961877	24.797253678755226	24.793573415224223
38	24.82786374869676	24.698203459860217	25.225351874734525	25.2485809167085
39	25.507925600174243	24.53475975835562	24.717145396286043	25.240169245184095
40	25.651472234037097	24.73117890335834	25.176906393003982	24.440442469600576
41	24.774584266720883	25.00607845704326	25.44831260222517	24.77102467401069
42	25.838238239687634	25.081265791626546	25.037706844667106	24.042789124018718
43	25.10984698343864	24.345819962558814	25.275092562259694	25.26924049174285
44	24.678469685836017	24.876835700090858	24.930770984988616	25.513923629084513
45	24.89117876847246	25.097328261545005	24.86342671497133	25.14806625501121
46	24.726069628569604	24.81614297832086	24.99321282528666	25.46457456782288
47	25.078777282459768	24.71437767605961	25.27201766976105	24.934827371719575
48	25.627171162735696	25.13016418484943	24.400158791255862	24.842505861159005
49	24.910228844051574	25.245422756988035	24.7165692939209	25.127779105039494
50	24.913199629088666	25.52036483002813	24.508684260219518	25.05775128066368
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	93.0
1	75.0
2	57.0
3	157.5
4	258.0
5	281.0
6	304.0
7	277.0
8	250.0
9	364.5
10	479.0
11	694.5
12	910.0
13	1747.5
14	2585.0
15	3359.0
16	4133.0
17	4463.5
18	4794.0
19	4751.0
20	4708.0
21	5210.0
22	5712.0
23	6418.5
24	7125.0
25	8319.5
26	9514.0
27	12091.5
28	14669.0
29	17468.0
30	20267.0
31	23370.0
32	26473.0
33	30925.5
34	35378.0
35	41272.0
36	47166.0
37	54734.5
38	62303.0
39	67479.0
40	72655.0
41	78691.5
42	84728.0
43	87157.5
44	89587.0
45	97974.5
46	106362.0
47	113009.5
48	119657.0
49	124181.0
50	128705.0
51	125189.0
52	121673.0
53	120477.0
54	119281.0
55	119300.0
56	119319.0
57	115827.0
58	112335.0
59	102555.5
60	92776.0
61	81877.0
62	70978.0
63	61863.5
64	52749.0
65	44734.0
66	36719.0
67	32287.0
68	27855.0
69	24877.0
70	21899.0
71	16978.5
72	12058.0
73	10074.0
74	8090.0
75	6368.5
76	4647.0
77	4030.5
78	3414.0
79	2784.5
80	2155.0
81	1680.5
82	1206.0
83	1012.5
84	819.0
85	566.5
86	314.0
87	230.0
88	146.0
89	112.5
90	79.0
91	66.0
92	53.0
93	46.5
94	40.0
95	43.5
96	47.0
97	34.5
98	22.0
99	18.5
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008747792690585746
2	4.8263683810128254E-4
3	2.4131841905064127E-4
4	0.0
5	0.0
6	1.8098881428798094E-4
7	0.0
8	0.0
9	0.0010256032809652254
10	7.239552571519238E-4
11	6.032960476266032E-5
12	2.4131841905064127E-4
13	6.032960476266032E-4
14	1.2065920952532063E-4
15	0.004585049961962184
16	4.8263683810128254E-4
17	0.0
18	3.619776285759619E-4
19	0.0
20	4.8263683810128254E-4
21	0.0
22	2.4131841905064127E-4
23	6.032960476266032E-4
24	3.619776285759619E-4
25	0.0023528545857437523
26	0.00567098284769007
27	0.007420541385807219
28	0.004766038776250165
29	0.002895821028607695
30	0.004102413123860902
31	0.008506474271535105
32	0.0037404354952849397
33	0.004464390752436863
34	0.0031974690524209973
35	0.0030164802381330157
36	0.008506474271535105
37	0.004283401938148883
38	0.009230429528687028
39	0.005067686800063467
40	0.002775161819082375
41	0.004102413123860902
42	0.0022321953762184316
43	0.0016892289333544888
44	0.001146262490490546
45	0.0015685697238291682
46	0.0013875809095411874
47	0.002413184190506413
48	0.0023528545857437523
49	7.842848619145841E-4
50	0.001327251304778527
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1657561.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.50846008897426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.37726248169703	39.57943978636326
2	14.148884995356259	14.85872326164302
3	4.634356238770371	7.3002872880468885
4	1.9642628042469004	4.125616602442202
5	1.0216738889842514	2.6823261311838342
6	0.6263369797756078	1.973279418287771
7	0.39267547404345177	1.4433149119710746
8	0.28549694503406964	1.199280395507642
9	0.21403461759821046	1.0114765358233053
>10	1.1743159599254251	11.205428173378287
>50	0.08476892123603007	3.0703242192153444
>100	0.06609238654005738	7.25894138218545
>500	0.007523411076503741	2.7251870806339222
>1k	0.002314895715847305	1.5663748133179767
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1736	0.10473219386797832	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1667	0.10056945113935474	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	6.032960476266032E-5
3	0.0	0.0	0.0	0.0	6.032960476266032E-5
4	0.0	0.0	0.0	0.0	6.032960476266032E-5
5	0.0	0.0	0.0	0.0	6.032960476266032E-5
6	0.0	0.0	0.0	0.0	1.2065920952532063E-4
7	0.0	0.0	0.0	6.032960476266032E-5	1.2065920952532063E-4
8	0.0	0.0	0.0	6.032960476266032E-5	1.2065920952532063E-4
9	0.0	0.0	0.0	1.2065920952532063E-4	1.2065920952532063E-4
10	0.0	0.0	0.0	1.2065920952532063E-4	1.2065920952532063E-4
11	0.0	0.0	0.0	1.2065920952532063E-4	1.2065920952532063E-4
12	0.0	0.0	0.0	1.2065920952532063E-4	2.4131841905064127E-4
13	0.0	0.0	0.0	1.8098881428798097E-4	2.4131841905064127E-4
14	0.0	0.0	0.0	1.8098881428798097E-4	2.4131841905064127E-4
15	0.0	0.0	0.0	1.8098881428798097E-4	2.4131841905064127E-4
16	0.0	0.0	0.0	1.8098881428798097E-4	2.4131841905064127E-4
17	0.0	0.0	0.0	1.8098881428798097E-4	3.016480238133016E-4
18	0.0	0.0	0.0	3.016480238133016E-4	4.223072333386222E-4
19	0.0	0.0	0.0	3.6197762857596193E-4	4.223072333386222E-4
20	0.0	0.0	0.0	3.6197762857596193E-4	4.8263683810128254E-4
21	0.0	0.0	0.0	4.8263683810128254E-4	6.032960476266032E-4
22	0.0	0.0	0.0	5.429664428639429E-4	6.636256523892635E-4
23	0.0	0.0	0.0	0.0015685697238291682	6.636256523892635E-4
24	0.0	0.0	0.0	0.0025338434000317335	6.636256523892635E-4
25	0.0	0.0	0.0	0.0028958210286076955	6.636256523892635E-4
26	0.0	0.0	0.0	0.003438787471471638	7.842848619145841E-4
27	0.0	0.0	0.0	0.003981753914335581	7.842848619145841E-4
28	0.0	0.0	0.0	0.006032960476266032	7.842848619145841E-4
29	0.0	0.0	0.0	0.009652736762025651	7.842848619145841E-4
30	0.0	6.032960476266032E-5	0.0	0.01665097091449425	7.842848619145841E-4
31	0.0	6.032960476266032E-5	0.0	0.038852265467153244	7.842848619145841E-4
32	0.0	6.032960476266032E-5	0.0	0.06045026397218564	7.842848619145841E-4
33	0.0	6.032960476266032E-5	0.0	0.08066068156767685	7.842848619145841E-4
34	0.0	6.032960476266032E-5	0.0	0.1035256017727251	7.842848619145841E-4
35	0.0	6.032960476266032E-5	0.0	0.1345350186207325	7.842848619145841E-4
36	0.0	6.032960476266032E-5	0.0	0.17839464128318655	7.842848619145841E-4
37	0.0	6.032960476266032E-5	0.0	0.23999116774586274	7.842848619145841E-4
38	0.0	6.032960476266032E-5	0.0	0.32390964797072325	8.446144666772444E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3335	0.0	21.571718	1
TAGGACG	485	0.0	20.864578	4
GTATAAT	185	1.8189894E-12	19.02747	1
ATGCGTA	110	2.7978804E-6	17.999876	22
CGTCGTA	405	0.0	17.925262	10
AACGCAG	4105	0.0	17.309435	6
CCGTCGT	395	0.0	17.265184	9
CTATTCC	515	0.0	17.08623	4
TCGATCG	130	7.3236697E-7	16.92245	41
GGTATCA	1725	0.0	16.835176	1
ATACCGT	420	0.0	16.760778	6
AATCGTA	145	1.2505734E-7	16.689037	13
CGAGCCG	650	0.0	16.584002	15
GTATTAC	160	2.1300366E-8	16.500385	1
TGCGTAT	120	6.4098385E-6	16.499886	23
TACCGTC	415	0.0	16.43263	7
AGGACGT	590	0.0	16.405678	5
GGACGTG	590	0.0	16.405678	6
TACGACG	405	0.0	16.295202	5
TATGGCG	505	0.0	16.11773	16
>>END_MODULE