FastQCFastQC Report
Thu 2 Feb 2017
SRR4063965_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063965_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences739794
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT82991.1217987710092268No Hit
TATCAACGCAGAGTACTTTTTTTTT61240.8277980086348361No Hit
GTACTTTTTTTTTTTTTTTTTTTTT44220.597733963779106No Hit
ACGCAGAGTACTTTTTTTTTTTTTT39980.5404207117116386No Hit
GTACATGGGGTGGTATCAACGCAAA37540.5074385572199829No Hit
CCATAGGGTCTTCTCGTCTTATTAT34570.4672922462198937No Hit
GGTATCAACGCAGAGTACTTTTTTT31320.4233610978191226No Hit
GAGTACTTTTTTTTTTTTTTTTTTT29360.39686723601435No Hit
GTATTAGAGGCACTGCCTGCCCAGT27800.37578028478197983No Hit
GTATCAACGCAGAGTACATGGGGTG26040.3519898782634085No Hit
CCATTGGGATGTCCTGATCCAACAT24350.3291456810950075No Hit
GTTCTAAGTTGGTCGTTAAGCGCCC20750.28048348594338424No Hit
CTTCTACACCATTGGGATGTCCTGA20430.2761579574854621No Hit
CTCTAATACTTGTAATGCTAGAGGT19840.26818276439116834No Hit
GTATCAACGCAGAGTACATGGGCAG19620.26520896357634693No Hit
GTCAGGATACCGCGGCCGTTAAACT19220.2598020530039443No Hit
TATCAACGCAGAGTACATGGGGTGG19160.2589910164180839No Hit
GATATATTTTGATCAACGGACCAAG17950.24263511193656612No Hit
ATGTAGATAAGGGAAGTCGGCAAAA17770.240202002178985No Hit
ATCCTGACCGTGCAAAGGTAGCATA17660.23871510177157426No Hit
GTATCAACGCAGAGTACATGGGAGA17320.23411922778503205No Hit
GTACATGGGAGAAATCGTAAATAGA17050.2304695631486603No Hit
ACCTATAACTTCTCTGTTAACCCAA16700.22573851639780804No Hit
GTACATGGGTGGTATCAACGCAAAA16410.22181850623281615No Hit
ACGCAGAGTACATGGGGTGGTATCA16390.22154816070419603No Hit
GTGCTGGGAAGCCCCGGCCGGTTGC16290.22019643306109538No Hit
GTATCCTGACCGTGCAAAGGTAGCA16170.21857435988937463No Hit
CCTCTAGCATTACAAGTATTAGAGG15860.2143840041957626No Hit
TCCCAATGGTGTAGAAGCTATTAAT15860.2143840041957626No Hit
ATCGTAAATAGATAGAAACCGACCT15670.21181572167387136No Hit
GTTATATAATTTAAGCTCCATAGGG15510.20965295744491036No Hit
GGTATCAACGCAGAGTACATGGGGT15380.2078957115088795No Hit
GCCTAAAGGAAAGATCCAAAAAGAT15270.20640881110146878No Hit
CCTATAACTTCTCTGTTAACCCAAC15210.2055977745156084No Hit
GCAGAGTACTTTTTTTTTTTTTTTT14770.19965017288596557No Hit
GATTAAAGATAAGAGACAGTTGGAC14650.19802809971424476No Hit
GATAGAAACCGACCTGGATTGCTCC14490.19586533548528376No Hit
GTTCATGCTAGTCCCTAATTAAGGA14490.19586533548528376No Hit
ATAAATAATCCACCTATAACTTCTC14350.19397291678494286No Hit
CTGTTAGTATGAGTAACAAGAATTC14280.19302670743477238No Hit
TCCCAGCACCGGGGCGGGGACGCCC13930.18829566068392012No Hit
CTGTTAACCCAACACCGGAATGCCT13800.18653841474788926No Hit
TGCTAGAGGTGATGTTTTTGGTAAA13780.18626806921926914No Hit
GGTCAGGATACCGCGGCCGTTAAAC13540.1830239228758276No Hit
GTGTAGAAGCTATTAATGGTTCGTT13370.1807259858825565No Hit
ATCTATAACTTTATAGATGCAACAC13170.1780225305963552No Hit
CACTATAAATAATCCACCTATAACT12740.1722101017310224No Hit
GACCTGGATTGCTCCGGTCTGAACT12210.165045945222589No Hit
ACTAGAGGCTGTTCACCTTGGAGAC12130.16396456310810847No Hit
GGATTGCTCCGGTCTGAACTCAGAT11900.160855589528977No Hit
GTACATGGGCAGAAATCACATTGCG11900.160855589528977No Hit
ACTTAGAACTGGTACGGACAAGGGG11650.1574762704212254No Hit
GACTATAGGCAATAATCACACTATA11600.15680040659967504No Hit
GTACATGGGAGTGGTATCAACGCAA11500.1554486789565744No Hit
TATCAACGCAGAGTACATGGGAGAA11410.1542321240777838No Hit
AGGGATAACAGCGCAATCCTATTTA11380.15382660578485363No Hit
ACCTTTGCACGGTCAGGATACCGCG11360.1535562602562335No Hit
GATTAAACCTTGTACCTTTTGCATA11290.15261005090606303No Hit
TATCTACATTGTTCTATCAACTAGA11220.15166384155589258No Hit
GTCCTGATCCAACATCGAGGTCGTA11150.15071763220572215No Hit
GCCTCTAGTTGATAGAACAATGTAG10930.1477438313909007No Hit
CTATAGAACTAGTACCGCAAGGGAA10740.1451755488690095No Hit
GTTTTAATTAGACAGTCAGATTCCC10530.14233692081849814No Hit
ACGCAGAGTACATGGGCAGAAATCA10420.1408500204110874No Hit
TATCAACGCAGAGTACATGGGCAGA10370.14017415658953708No Hit
ATCAACGCAGAGTACTTTTTTTTTT10230.13828173788919618No Hit
GTTATAGATTAACCCAATTTTAAGT10050.13584862813161502No Hit
ATTTAAGAGTTCATATCGACAATTA10040.13571345536730495No Hit
GGTATCAACGCAGAGTACATGGGAG10000.1351727643100647No Hit
ATCCTAAGGCATAGGGAAGTTCCGT9960.13463207325282442No Hit
AGCATGAACGGCTAAACGAGGGTCC9960.13463207325282442No Hit
CCATTAATAGCTTCTACACCATTGG9930.13422655495989425No Hit
CAGTTGGACCCTCGTTTAGCCGTTC9920.13409138219558417No Hit
CCCCTTGTCCGTACCAGTTCTAAGT9500.12841412609456146No Hit
CCTATAGTCTGATTAACTAACAATG9420.12733274398008093No Hit
ATTTCAGCCTCTTCACTGAAAGGTC9340.12625136186560043No Hit
GTTAGTATGAGTAACAAGAATTCCA9320.1259810163369803No Hit
TCGCAATGCTATGTTTTAATTAGAC9310.12584584357267023No Hit
GTATCAACGCAGAGTACATGGGTGG9050.12233135170060855No Hit
GGGCAGGCAGTGCCTCTAATACTTG8920.12057410576457771No Hit
AAATATATCTGGGTCAATAAGATAT8820.11922237812147707No Hit
ATTATAACCTAGACTTACAAGTCAA8800.11895203259285694No Hit
GTTTAAAATTGAACTTAAATTCATT8720.11787065047837642No Hit
GTCCGTACCAGTTCTAAGTTGGTCG8710.11773547771406635No Hit
ACGCAGAGTACATGGGAGAAATCGT8640.1167892683638959No Hit
GTATAAGTTTGAAATTTCGGTTGGG8580.1159782317780355No Hit
GGATACCGCGGCCGTTAAACTTTAG8540.11543754072079525No Hit
CTTAAATAGGATTGCGCTGTTATCC8480.11462650413493487No Hit
AGATAAGGGAAGTCGGCAAAATGGA8440.1140858130776946No Hit
TGATAGAACAATGTAGATAAGGGAA8410.11368029478476441No Hit
GGTATCAACGCAGAGTACATGGGCA8410.11368029478476441No Hit
GATAAAAGGAACTCGGCAAACAAGA8390.11340994925614428No Hit
CAATTAGGGTTTACGACCTCGATGT8330.1125989126702839No Hit
GTCGTACTCATAACCGCAGCAGGTC8320.11246373990597383No Hit
GCTATCACCAAGCTCGTTAGGCTTT8300.1121933943773537No Hit
ACTAAAAGTATTGGAGAAAGAAATT8140.11003063014839266No Hit
GGGATGTCCTGATCCAACATCGAGG8090.10935476632684234No Hit
GTCAGATTCCCCTTGTCCGTACCAG7990.1080030386837417No Hit
CTATAGTATAAGTTTGAAATTTCGG7920.10705682933357123No Hit
GTTCTATCAACTAGAGGCTGTTCAC7790.10529958339754039No Hit
GTTATCCCTAGGGTAACTTGGTCCG7780.10516441063323032No Hit
GAACAATGTAGATAAGGGAAGTCGG7510.10151474599685859No Hit
CTATTAAAGGTTTTTTCCGTTCCAG7470.10097405493961834No Hit
GTATCAACGCAGAGTACATGGGAGT7450.10070370941099818No Hit
CTTCACTGAAAGGTCAATTTCACTG7400.10002784558944787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACAAA307.7156455E-419.0006771
CCTAGAG250.006042056718.9929682
TTGCTAA753.270543E-916.46725310
CGCACCA350.002168236316.28629510
ACTTGCT755.8236765E-815.201578
AACTTGC755.8236765E-815.201577
TGGTCGT3600.015.04220210
GGTCGTT3600.015.04016811
TTAGAAC1650.014.9661813
CTTAGAA1650.014.9641562
GATCTAG456.754575E-414.7783046
CTCTACT1101.2732926E-1114.6823421
AGCGCCC3650.014.57783419
CAGCACC3550.014.451224
AAGCGCC3700.014.37986318
GTTAAGC3700.014.3788915
TAAGCGC3700.014.3788917
AATACTG400.00527439414.2505075
GCTTTGA400.00527439414.2505071
GTCGTTA3800.014.2485812
Produced by FastQC (version 0.11.5)