##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063965_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	739794
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.085611670275778	32.0	32.0	32.0	32.0	32.0
2	30.860873432333864	32.0	32.0	32.0	32.0	32.0
3	30.928908588066406	32.0	32.0	32.0	32.0	32.0
4	30.90028440349611	32.0	32.0	32.0	32.0	32.0
5	30.907236338764577	32.0	32.0	32.0	32.0	32.0
6	34.49997972408536	36.0	36.0	36.0	32.0	36.0
7	34.33977431555271	36.0	36.0	36.0	32.0	36.0
8	34.25084009873019	36.0	36.0	36.0	32.0	36.0
9	34.32904294979413	36.0	36.0	36.0	32.0	36.0
10	34.177781923075884	36.0	36.0	36.0	32.0	36.0
11	34.46649337518282	36.0	36.0	36.0	32.0	36.0
12	34.30656101563408	36.0	36.0	36.0	32.0	36.0
13	34.375081441590496	36.0	36.0	36.0	32.0	36.0
14	34.30180023087508	36.0	36.0	36.0	32.0	36.0
15	34.28401825373009	36.0	36.0	36.0	32.0	36.0
16	34.26254606011944	36.0	36.0	36.0	32.0	36.0
17	34.20874459646875	36.0	36.0	36.0	32.0	36.0
18	34.22157789871235	36.0	36.0	36.0	32.0	36.0
19	34.183991759868285	36.0	36.0	36.0	32.0	36.0
20	34.07586436224138	36.0	36.0	36.0	32.0	36.0
21	34.04004222797157	36.0	36.0	36.0	32.0	36.0
22	34.07829341681603	36.0	36.0	36.0	32.0	36.0
23	34.10559831520666	36.0	36.0	36.0	32.0	36.0
24	34.08553732525541	36.0	36.0	36.0	32.0	36.0
25	33.78223262151356	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	7.0
5	8.0
6	31.0
7	17.0
8	28.0
9	53.0
10	67.0
11	14.0
12	48.0
13	38.0
14	175.0
15	233.0
16	320.0
17	398.0
18	530.0
19	724.0
20	1053.0
21	1504.0
22	2258.0
23	3089.0
24	4307.0
25	5903.0
26	8053.0
27	10600.0
28	14283.0
29	19126.0
30	24710.0
31	33520.0
32	46757.0
33	66381.0
34	147844.0
35	347715.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.485180807936025	21.62420184066833	12.9978607572	21.892756594195646
2	15.209756651463579	21.36984597749006	39.39807667871559	24.022320692330776
3	17.394019597638767	27.666761739591205	31.483691839363704	23.455526823406323
4	9.483932102945927	17.85278337638743	39.734808392372194	32.92847612829445
5	10.888161896908331	41.171643707839344	34.20234409852244	13.737850296729887
6	27.574235675032615	41.526824882892505	18.79533057097086	12.103608871104022
7	26.594207111383923	36.46777920341359	20.053648373310477	16.88436531189201
8	28.90076412261119	31.573232586158174	19.473700579143006	20.052302712087634
9	26.711087549801064	15.285309733555843	22.273416981994533	35.730185734648565
10	15.620287302920502	27.86481262823608	31.652282585320528	24.86261748352289
11	32.707637376756516	24.81626746372009	24.126644937042695	18.349450222480694
12	23.711720874997802	29.07002065358381	28.8967577795842	18.321500691834192
13	25.2267009333153	23.154199918842146	28.97213580413905	22.6469633437035
14	22.29719036301684	23.99831186415917	27.68515725499373	26.01934051783026
15	24.539507229793976	28.146627335938252	26.71297630012538	20.6008891341424
16	23.8986556219997	29.54412343551544	25.21543998993644	21.34178095254842
17	19.210870514972523	27.58788131271802	28.7319040038089	24.469344168500555
18	19.159009988853175	28.753260175535427	29.903385171477115	22.18434466413428
19	22.87564016892614	27.439270187133584	29.704243985185148	19.98084565875513
20	23.20629956197428	28.312045301436907	28.787407369329543	19.694247767259267
21	25.053940168685617	24.842778781053642	29.131343465292293	20.971937584968448
22	22.76687156400156	26.99301437167222	28.596976321371372	21.643137742954853
23	23.238450580565562	26.51662015197655	29.403552252253473	20.84137701520442
24	22.227558097536495	29.270552777142733	27.625600116878214	20.87628900844256
25	22.035629399056575	28.307231531571748	29.31748421649277	20.33965485287891
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	300.0
1	300.0
2	830.0
3	1360.0
4	1360.0
5	1360.0
6	3866.0
7	6372.0
8	6372.0
9	6372.0
10	5778.5
11	5185.0
12	5185.0
13	5185.0
14	5035.0
15	4885.0
16	4885.0
17	4885.0
18	7897.5
19	10910.0
20	10910.0
21	10910.0
22	18609.5
23	26309.0
24	26309.0
25	26309.0
26	39942.5
27	53576.0
28	53576.0
29	53576.0
30	60938.0
31	68300.0
32	68300.0
33	68300.0
34	71334.0
35	74368.0
36	74368.0
37	74368.0
38	72064.0
39	69760.0
40	69760.0
41	69760.0
42	67046.0
43	64332.0
44	64332.0
45	64332.0
46	83218.0
47	102104.0
48	102104.0
49	102104.0
50	96764.5
51	91425.0
52	91425.0
53	91425.0
54	71940.5
55	52456.0
56	52456.0
57	52456.0
58	40579.0
59	28702.0
60	28702.0
61	28702.0
62	23656.0
63	18610.0
64	18610.0
65	18610.0
66	17526.5
67	16443.0
68	16443.0
69	16443.0
70	15661.5
71	14880.0
72	14880.0
73	14880.0
74	14089.0
75	13298.0
76	13298.0
77	13298.0
78	11775.5
79	10253.0
80	10253.0
81	10253.0
82	7687.0
83	5121.0
84	5121.0
85	5121.0
86	2756.0
87	391.0
88	391.0
89	391.0
90	250.0
91	109.0
92	109.0
93	109.0
94	89.5
95	70.0
96	70.0
97	70.0
98	172.5
99	275.0
100	275.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03784837400681811
2	0.00432552845792207
3	0.0014869004074107117
4	0.003649664636371747
5	0.00865105691584414
6	0.012030376023595758
7	0.022033160582540547
8	0.03419870937044636
9	0.047580813037142775
10	0.058800152474878145
11	0.05460979678126614
12	0.06150360776107943
13	0.06677534556917196
14	0.06988431914830345
15	0.05717807930315736
16	0.0666401728048619
17	0.06393671751866059
18	0.07704847565673688
19	0.07245260167019468
20	0.07677813012811675
21	0.07420984760622552
22	0.07596709354225635
23	0.08123883135034889
24	0.07610226630656643
25	0.07745399394966707
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	739794.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.15381304828284
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.82411356679616	12.799365783756345
2	14.31564111491705	5.483982271628649
3	6.250519090950739	3.591638223683798
4	3.2121509594425737	2.4609975584010164
5	1.9759292661401355	1.892328988013943
6	1.2426611796373743	1.428101995027973
7	0.8834431722039888	1.1844913751424002
8	0.6135761327918545	0.9401858030706836
9	0.49617698871712423	0.8553313152672967
>10	3.009521680552638	11.33295832147107
>50	0.4565354832388789	6.243660424777017
>100	0.5884862334176584	23.929909463605192
>500	0.0832630402197646	10.932251108066831
>1k	0.046570853004275124	14.97548649711004
>5k	0.0014112379698265189	1.9493108709777458
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	8299	1.1217987710092268	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6124	0.8277980086348361	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	4422	0.597733963779106	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3998	0.5404207117116386	No Hit
GTACATGGGGTGGTATCAACGCAAA	3754	0.5074385572199829	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	3457	0.4672922462198937	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3132	0.4233610978191226	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2936	0.39686723601435	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	2780	0.37578028478197983	No Hit
GTATCAACGCAGAGTACATGGGGTG	2604	0.3519898782634085	No Hit
CCATTGGGATGTCCTGATCCAACAT	2435	0.3291456810950075	No Hit
GTTCTAAGTTGGTCGTTAAGCGCCC	2075	0.28048348594338424	No Hit
CTTCTACACCATTGGGATGTCCTGA	2043	0.2761579574854621	No Hit
CTCTAATACTTGTAATGCTAGAGGT	1984	0.26818276439116834	No Hit
GTATCAACGCAGAGTACATGGGCAG	1962	0.26520896357634693	No Hit
GTCAGGATACCGCGGCCGTTAAACT	1922	0.2598020530039443	No Hit
TATCAACGCAGAGTACATGGGGTGG	1916	0.2589910164180839	No Hit
GATATATTTTGATCAACGGACCAAG	1795	0.24263511193656612	No Hit
ATGTAGATAAGGGAAGTCGGCAAAA	1777	0.240202002178985	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	1766	0.23871510177157426	No Hit
GTATCAACGCAGAGTACATGGGAGA	1732	0.23411922778503205	No Hit
GTACATGGGAGAAATCGTAAATAGA	1705	0.2304695631486603	No Hit
ACCTATAACTTCTCTGTTAACCCAA	1670	0.22573851639780804	No Hit
GTACATGGGTGGTATCAACGCAAAA	1641	0.22181850623281615	No Hit
ACGCAGAGTACATGGGGTGGTATCA	1639	0.22154816070419603	No Hit
GTGCTGGGAAGCCCCGGCCGGTTGC	1629	0.22019643306109538	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	1617	0.21857435988937463	No Hit
CCTCTAGCATTACAAGTATTAGAGG	1586	0.2143840041957626	No Hit
TCCCAATGGTGTAGAAGCTATTAAT	1586	0.2143840041957626	No Hit
ATCGTAAATAGATAGAAACCGACCT	1567	0.21181572167387136	No Hit
GTTATATAATTTAAGCTCCATAGGG	1551	0.20965295744491036	No Hit
GGTATCAACGCAGAGTACATGGGGT	1538	0.2078957115088795	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	1527	0.20640881110146878	No Hit
CCTATAACTTCTCTGTTAACCCAAC	1521	0.2055977745156084	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	1477	0.19965017288596557	No Hit
GATTAAAGATAAGAGACAGTTGGAC	1465	0.19802809971424476	No Hit
GATAGAAACCGACCTGGATTGCTCC	1449	0.19586533548528376	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	1449	0.19586533548528376	No Hit
ATAAATAATCCACCTATAACTTCTC	1435	0.19397291678494286	No Hit
CTGTTAGTATGAGTAACAAGAATTC	1428	0.19302670743477238	No Hit
TCCCAGCACCGGGGCGGGGACGCCC	1393	0.18829566068392012	No Hit
CTGTTAACCCAACACCGGAATGCCT	1380	0.18653841474788926	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	1378	0.18626806921926914	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	1354	0.1830239228758276	No Hit
GTGTAGAAGCTATTAATGGTTCGTT	1337	0.1807259858825565	No Hit
ATCTATAACTTTATAGATGCAACAC	1317	0.1780225305963552	No Hit
CACTATAAATAATCCACCTATAACT	1274	0.1722101017310224	No Hit
GACCTGGATTGCTCCGGTCTGAACT	1221	0.165045945222589	No Hit
ACTAGAGGCTGTTCACCTTGGAGAC	1213	0.16396456310810847	No Hit
GGATTGCTCCGGTCTGAACTCAGAT	1190	0.160855589528977	No Hit
GTACATGGGCAGAAATCACATTGCG	1190	0.160855589528977	No Hit
ACTTAGAACTGGTACGGACAAGGGG	1165	0.1574762704212254	No Hit
GACTATAGGCAATAATCACACTATA	1160	0.15680040659967504	No Hit
GTACATGGGAGTGGTATCAACGCAA	1150	0.1554486789565744	No Hit
TATCAACGCAGAGTACATGGGAGAA	1141	0.1542321240777838	No Hit
AGGGATAACAGCGCAATCCTATTTA	1138	0.15382660578485363	No Hit
ACCTTTGCACGGTCAGGATACCGCG	1136	0.1535562602562335	No Hit
GATTAAACCTTGTACCTTTTGCATA	1129	0.15261005090606303	No Hit
TATCTACATTGTTCTATCAACTAGA	1122	0.15166384155589258	No Hit
GTCCTGATCCAACATCGAGGTCGTA	1115	0.15071763220572215	No Hit
GCCTCTAGTTGATAGAACAATGTAG	1093	0.1477438313909007	No Hit
CTATAGAACTAGTACCGCAAGGGAA	1074	0.1451755488690095	No Hit
GTTTTAATTAGACAGTCAGATTCCC	1053	0.14233692081849814	No Hit
ACGCAGAGTACATGGGCAGAAATCA	1042	0.1408500204110874	No Hit
TATCAACGCAGAGTACATGGGCAGA	1037	0.14017415658953708	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	1023	0.13828173788919618	No Hit
GTTATAGATTAACCCAATTTTAAGT	1005	0.13584862813161502	No Hit
ATTTAAGAGTTCATATCGACAATTA	1004	0.13571345536730495	No Hit
GGTATCAACGCAGAGTACATGGGAG	1000	0.1351727643100647	No Hit
ATCCTAAGGCATAGGGAAGTTCCGT	996	0.13463207325282442	No Hit
AGCATGAACGGCTAAACGAGGGTCC	996	0.13463207325282442	No Hit
CCATTAATAGCTTCTACACCATTGG	993	0.13422655495989425	No Hit
CAGTTGGACCCTCGTTTAGCCGTTC	992	0.13409138219558417	No Hit
CCCCTTGTCCGTACCAGTTCTAAGT	950	0.12841412609456146	No Hit
CCTATAGTCTGATTAACTAACAATG	942	0.12733274398008093	No Hit
ATTTCAGCCTCTTCACTGAAAGGTC	934	0.12625136186560043	No Hit
GTTAGTATGAGTAACAAGAATTCCA	932	0.1259810163369803	No Hit
TCGCAATGCTATGTTTTAATTAGAC	931	0.12584584357267023	No Hit
GTATCAACGCAGAGTACATGGGTGG	905	0.12233135170060855	No Hit
GGGCAGGCAGTGCCTCTAATACTTG	892	0.12057410576457771	No Hit
AAATATATCTGGGTCAATAAGATAT	882	0.11922237812147707	No Hit
ATTATAACCTAGACTTACAAGTCAA	880	0.11895203259285694	No Hit
GTTTAAAATTGAACTTAAATTCATT	872	0.11787065047837642	No Hit
GTCCGTACCAGTTCTAAGTTGGTCG	871	0.11773547771406635	No Hit
ACGCAGAGTACATGGGAGAAATCGT	864	0.1167892683638959	No Hit
GTATAAGTTTGAAATTTCGGTTGGG	858	0.1159782317780355	No Hit
GGATACCGCGGCCGTTAAACTTTAG	854	0.11543754072079525	No Hit
CTTAAATAGGATTGCGCTGTTATCC	848	0.11462650413493487	No Hit
AGATAAGGGAAGTCGGCAAAATGGA	844	0.1140858130776946	No Hit
TGATAGAACAATGTAGATAAGGGAA	841	0.11368029478476441	No Hit
GGTATCAACGCAGAGTACATGGGCA	841	0.11368029478476441	No Hit
GATAAAAGGAACTCGGCAAACAAGA	839	0.11340994925614428	No Hit
CAATTAGGGTTTACGACCTCGATGT	833	0.1125989126702839	No Hit
GTCGTACTCATAACCGCAGCAGGTC	832	0.11246373990597383	No Hit
GCTATCACCAAGCTCGTTAGGCTTT	830	0.1121933943773537	No Hit
ACTAAAAGTATTGGAGAAAGAAATT	814	0.11003063014839266	No Hit
GGGATGTCCTGATCCAACATCGAGG	809	0.10935476632684234	No Hit
GTCAGATTCCCCTTGTCCGTACCAG	799	0.1080030386837417	No Hit
CTATAGTATAAGTTTGAAATTTCGG	792	0.10705682933357123	No Hit
GTTCTATCAACTAGAGGCTGTTCAC	779	0.10529958339754039	No Hit
GTTATCCCTAGGGTAACTTGGTCCG	778	0.10516441063323032	No Hit
GAACAATGTAGATAAGGGAAGTCGG	751	0.10151474599685859	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	747	0.10097405493961834	No Hit
GTATCAACGCAGAGTACATGGGAGT	745	0.10070370941099818	No Hit
CTTCACTGAAAGGTCAATTTCACTG	740	0.10002784558944787	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.351727643100647E-4	0.0
8	0.0	0.0	0.0	2.703455286201294E-4	0.0
9	0.0	0.0	0.0	4.055182929301941E-4	0.0
10	0.0	0.0	0.0	4.055182929301941E-4	0.0
11	0.0	0.0	0.0	4.055182929301941E-4	1.351727643100647E-4
12	0.0	0.0	0.0	4.055182929301941E-4	1.351727643100647E-4
13	0.0	0.0	0.0	4.055182929301941E-4	1.351727643100647E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACAAA	30	7.7156455E-4	19.000677	1
CCTAGAG	25	0.0060420567	18.992968	2
TTGCTAA	75	3.270543E-9	16.467253	10
CGCACCA	35	0.0021682363	16.286295	10
ACTTGCT	75	5.8236765E-8	15.20157	8
AACTTGC	75	5.8236765E-8	15.20157	7
TGGTCGT	360	0.0	15.042202	10
GGTCGTT	360	0.0	15.040168	11
TTAGAAC	165	0.0	14.966181	3
CTTAGAA	165	0.0	14.964156	2
GATCTAG	45	6.754575E-4	14.778304	6
CTCTACT	110	1.2732926E-11	14.682342	1
AGCGCCC	365	0.0	14.577834	19
CAGCACC	355	0.0	14.45122	4
AAGCGCC	370	0.0	14.379863	18
GTTAAGC	370	0.0	14.37889	15
TAAGCGC	370	0.0	14.37889	17
AATACTG	40	0.005274394	14.250507	5
GCTTTGA	40	0.005274394	14.250507	1
GTCGTTA	380	0.0	14.24858	12
>>END_MODULE