FastQCFastQC Report
Thu 2 Feb 2017
SRR4063964_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063964_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3676904
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58640.15948199898610352No Hit
TATCAACGCAGAGTACTTTTTTTTT38770.10544196965708105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13950.013.8198881
CGTTATT2000.013.2942382
GTATCAA38450.012.9177861
GCGTTAT2050.012.0448711
AAGACGG5400.011.2568325
ACGAACG3050.010.90344315
GTCCTAA4250.010.7259311
CGTCTTA1701.382432E-1010.61940515
CGACCAT5200.010.59733110
CGAACGA3150.010.55730316
ACGGTAT3700.010.5279099
GAACCGT1002.4036475E-510.4488856
CGCGTAA2550.010.4325310
TCGCGTA2550.010.4322469
CGGACCA5100.010.4322469
CGCATCG3300.010.36521113
TAATACT5600.010.345424
CCGTCGT4600.010.3269529
CGGTCCA5550.010.27141110
CGAGCCG4750.010.20168815
Produced by FastQC (version 0.11.5)