##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063964_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3676904
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209352215885975	32.0	32.0	32.0	32.0	32.0
2	30.703055886147695	32.0	32.0	32.0	32.0	32.0
3	30.735286262573076	32.0	32.0	32.0	32.0	32.0
4	30.7761793617674	32.0	32.0	32.0	32.0	32.0
5	30.633819376301368	32.0	32.0	32.0	32.0	32.0
6	34.30683721957386	36.0	36.0	36.0	32.0	36.0
7	34.2516541090004	36.0	36.0	36.0	32.0	36.0
8	34.193016733643304	36.0	36.0	36.0	32.0	36.0
9	34.385287459232	36.0	36.0	36.0	32.0	36.0
10	33.988188704410014	36.0	36.0	36.0	32.0	36.0
11	34.36030502836082	36.0	36.0	36.0	32.0	36.0
12	34.13086988401111	36.0	36.0	36.0	32.0	36.0
13	34.23545297891922	36.0	36.0	36.0	32.0	36.0
14	34.114048667030744	36.0	36.0	36.0	32.0	36.0
15	34.030525681388475	36.0	36.0	36.0	32.0	36.0
16	34.03905378002798	36.0	36.0	36.0	32.0	36.0
17	33.95960106654947	36.0	36.0	36.0	32.0	36.0
18	33.95267540300209	36.0	36.0	36.0	32.0	36.0
19	33.97924558269675	36.0	36.0	36.0	32.0	36.0
20	33.95904054062875	36.0	36.0	36.0	32.0	36.0
21	33.950910875018764	36.0	36.0	36.0	32.0	36.0
22	33.93019398929099	36.0	36.0	36.0	32.0	36.0
23	33.85650781200706	36.0	36.0	36.0	32.0	36.0
24	33.845184154930344	36.0	36.0	36.0	32.0	36.0
25	33.40932670529337	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	12.0
4	32.0
5	50.0
6	152.0
7	60.0
8	213.0
9	231.0
10	302.0
11	101.0
12	217.0
13	198.0
14	320.0
15	554.0
16	950.0
17	1341.0
18	1822.0
19	2564.0
20	4047.0
21	6177.0
22	9801.0
23	15204.0
24	22650.0
25	32749.0
26	46567.0
27	61191.0
28	82688.0
29	110625.0
30	143588.0
31	193568.0
32	268955.0
33	374849.0
34	770884.0
35	1524241.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.66951456881245	16.874568420663135	11.190644747560437	26.26527226296398
2	16.908730903443566	19.26567501924275	37.16746765450634	26.65812642280735
3	18.873422462089184	23.100977928679768	28.413510140329244	29.612089468901804
4	12.643495591780606	15.444651389481196	35.565502093142676	36.34635092559552
5	14.80277464307192	36.23494219221609	33.83662701841738	15.125656146294613
6	34.91313970842839	35.27433338020863	16.548621607808244	13.263905303554735
7	30.674328101120924	30.245909086705	20.561691630799597	18.518071181374477
8	28.413591028549973	33.06984323903735	18.90294532138446	19.613620411028222
9	27.243570644343677	14.067347185885689	18.11634130446909	40.57274086530155
10	15.641048387623464	26.913615454780963	31.9023713788742	25.542964778721377
11	37.273801136841385	20.92086927630242	22.324383204092392	19.48094638276381
12	24.541188406254612	23.493401462253598	29.007413592002617	22.957996539489177
13	29.646132455878476	19.55375966746558	24.855799336357183	25.94430854029876
14	23.63465600848242	19.301103683790267	25.061900892167532	32.00233941555978
15	25.340508102774383	27.12722489195802	21.93560395882254	25.596663046445055
16	25.960106802058856	25.675085407084197	23.46708809809112	24.897719692765826
17	24.280678226613638	25.75404624450491	24.985399938279702	24.97987559060175
18	24.88825670663895	25.107959998323377	25.694016895814585	24.30976639922309
19	25.572897317084788	24.9477501752094	25.015384406023124	24.46396810168269
20	25.860637666643076	24.52713920991176	24.628798972242937	24.983424151202225
21	26.657414579685074	24.37028561350373	24.278617554215614	24.693682252595583
22	26.100776147482176	24.26348187446857	24.696683001074565	24.93905897697469
23	24.936960928840975	24.2942672551765	25.06377398261036	25.70499783337217
24	24.935011206841413	24.908446842011433	24.897804765527294	25.25873718561986
25	25.16192511040194	24.417220660982697	24.954907017412545	25.46594721120282
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	108.0
1	108.0
2	413.0
3	718.0
4	718.0
5	718.0
6	1966.0
7	3214.0
8	3214.0
9	3214.0
10	4020.0
11	4826.0
12	4826.0
13	4826.0
14	7006.0
15	9186.0
16	9186.0
17	9186.0
18	15579.5
19	21973.0
20	21973.0
21	21973.0
22	34705.0
23	47437.0
24	47437.0
25	47437.0
26	71257.5
27	95078.0
28	95078.0
29	95078.0
30	120933.0
31	146788.0
32	146788.0
33	146788.0
34	183465.0
35	220142.0
36	220142.0
37	220142.0
38	258535.0
39	296928.0
40	296928.0
41	296928.0
42	344895.5
43	392863.0
44	392863.0
45	392863.0
46	433070.5
47	473278.0
48	473278.0
49	473278.0
50	488327.0
51	503376.0
52	503376.0
53	503376.0
54	477050.5
55	450725.0
56	450725.0
57	450725.0
58	412880.5
59	375036.0
60	375036.0
61	375036.0
62	326976.5
63	278917.0
64	278917.0
65	278917.0
66	228292.5
67	177668.0
68	177668.0
69	177668.0
70	133730.5
71	89793.0
72	89793.0
73	89793.0
74	67992.0
75	46191.0
76	46191.0
77	46191.0
78	35333.5
79	24476.0
80	24476.0
81	24476.0
82	16786.0
83	9096.0
84	9096.0
85	9096.0
86	6790.5
87	4485.0
88	4485.0
89	4485.0
90	3167.5
91	1850.0
92	1850.0
93	1850.0
94	1337.5
95	825.0
96	825.0
97	825.0
98	1376.0
99	1927.0
100	1927.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.039435351045335965
2	0.005276178001927708
3	0.0013870364850428513
4	0.00369876396011427
5	0.008784564405271391
6	0.013136051417170534
7	0.022573338874226793
8	0.033832811517515826
9	0.04811112827530988
10	0.05656933115468884
11	0.053985635741373716
12	0.06260701938369889
13	0.06355890716755183
14	0.06750244227208543
15	0.059153026568003954
16	0.06644176731293501
17	0.06151914763072411
18	0.07772843675004841
19	0.07422005034670473
20	0.07843555338948202
21	0.07555269324409884
22	0.07778283033769715
23	0.08137280712251393
24	0.0763957938526543
25	0.07693972972914169
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3676904.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.81063853498889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.90205797299788	27.972177631558864
2	15.954980436866492	13.341758397572882
3	6.601507910640468	8.28039783112975
4	3.328719588304115	5.567035659636815
5	1.9002833006358397	3.9726029098480358
6	1.1994862337198327	3.0090771207453075
7	0.8157148358626573	2.387389070491695
8	0.5781665820612246	1.9338811180457482
9	0.42235432161176467	1.5893013487139944
>10	2.077978046659494	15.360201739524953
>50	0.12530166886474048	3.6024457467480064
>100	0.07843861610953523	6.527392471010754
>500	0.010028839775633477	2.8753031956001607
>1k	0.004916097917990919	3.420326824432264
>5k	6.554797223987892E-5	0.16070893494071248
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5864	0.15948199898610352	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3877	0.10544196965708105	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3598396912184818E-4
2	0.0	0.0	0.0	0.0	1.3598396912184818E-4
3	0.0	0.0	0.0	0.0	1.3598396912184818E-4
4	0.0	0.0	0.0	0.0	1.3598396912184818E-4
5	0.0	0.0	0.0	0.0	1.631807629462178E-4
6	0.0	0.0	0.0	0.0	3.5355831971680524E-4
7	0.0	0.0	0.0	2.7196793824369633E-5	3.5355831971680524E-4
8	0.0	0.0	0.0	2.7196793824369633E-5	3.5355831971680524E-4
9	0.0	0.0	0.0	2.7196793824369633E-5	3.5355831971680524E-4
10	0.0	0.0	0.0	2.7196793824369633E-5	3.8075511354117487E-4
11	0.0	0.0	0.0	2.7196793824369633E-5	3.8075511354117487E-4
12	0.0	0.0	0.0	2.7196793824369633E-5	7.343134332579801E-4
13	0.0	0.0	0.0	2.7196793824369633E-5	9.518877838529371E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1395	0.0	13.819888	1
CGTTATT	200	0.0	13.294238	2
GTATCAA	3845	0.0	12.917786	1
GCGTTAT	205	0.0	12.044871	1
AAGACGG	540	0.0	11.256832	5
ACGAACG	305	0.0	10.903443	15
GTCCTAA	425	0.0	10.725931	1
CGTCTTA	170	1.382432E-10	10.619405	15
CGACCAT	520	0.0	10.597331	10
CGAACGA	315	0.0	10.557303	16
ACGGTAT	370	0.0	10.527909	9
GAACCGT	100	2.4036475E-5	10.448885	6
CGCGTAA	255	0.0	10.43253	10
TCGCGTA	255	0.0	10.432246	9
CGGACCA	510	0.0	10.432246	9
CGCATCG	330	0.0	10.365211	13
TAATACT	560	0.0	10.34542	4
CCGTCGT	460	0.0	10.326952	9
CGGTCCA	555	0.0	10.271411	10
CGAGCCG	475	0.0	10.201688	15
>>END_MODULE