FastQCFastQC Report
Thu 2 Feb 2017
SRR4063964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3676904
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40700.027.406551
GGTATCA18400.023.5553281
TCAACGC62850.017.5717744
CAACGCA64150.017.2156835
TATCAAC64650.017.1498952
AACGCAG64500.017.1222636
ATCAACG64550.017.1090013
CGCAATA8300.016.69926536
CGTCGTA7750.016.46408510
TATAACG1259.455474E-615.8391522
CTAGCGG8350.015.80839829
TAGGACG4900.015.71408754
ACTAACG850.002981740315.5292153
ACCGTCG8250.015.1998088
CAATACG9150.015.14776138
GTATAGA5150.014.952081
TCTAGCG9000.014.91132728
ACTATAC3700.014.86467553
CCGTCGT8150.014.8470439
ATACCGT8450.014.8400496
Produced by FastQC (version 0.11.5)