##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063964_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3676904
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.342390228300765	32.0	32.0	32.0	32.0	32.0
2	31.397615765872594	32.0	32.0	32.0	32.0	32.0
3	31.47732793676419	32.0	32.0	32.0	32.0	32.0
4	31.562639655536287	32.0	32.0	32.0	32.0	32.0
5	31.513472747724716	32.0	32.0	32.0	32.0	32.0
6	35.10098631892483	36.0	36.0	36.0	36.0	36.0
7	35.12027510100889	36.0	36.0	36.0	36.0	36.0
8	35.06387901343086	36.0	36.0	36.0	36.0	36.0
9	35.173652072504474	36.0	36.0	36.0	36.0	36.0
10	35.027814976948	36.0	36.0	36.0	36.0	36.0
11	35.172651774427614	36.0	36.0	36.0	36.0	36.0
12	35.082302937471304	36.0	36.0	36.0	36.0	36.0
13	35.126213520940446	36.0	36.0	36.0	36.0	36.0
14	35.064032131380095	36.0	36.0	36.0	36.0	36.0
15	35.04042096285353	36.0	36.0	36.0	36.0	36.0
16	35.05162631387711	36.0	36.0	36.0	36.0	36.0
17	35.01977750846908	36.0	36.0	36.0	36.0	36.0
18	35.01697487886548	36.0	36.0	36.0	36.0	36.0
19	35.01587803217054	36.0	36.0	36.0	36.0	36.0
20	35.010908090067076	36.0	36.0	36.0	36.0	36.0
21	35.00964643080157	36.0	36.0	36.0	36.0	36.0
22	34.99078055886147	36.0	36.0	36.0	36.0	36.0
23	34.937247205801405	36.0	36.0	36.0	36.0	36.0
24	34.91328438273069	36.0	36.0	36.0	32.0	36.0
25	34.88891007216941	36.0	36.0	36.0	32.0	36.0
26	34.83484039833512	36.0	36.0	36.0	32.0	36.0
27	34.82762182531825	36.0	36.0	36.0	32.0	36.0
28	34.80453093145755	36.0	36.0	36.0	32.0	36.0
29	34.78483963682489	36.0	36.0	36.0	32.0	36.0
30	34.7673433954218	36.0	36.0	36.0	32.0	36.0
31	34.76108949268189	36.0	36.0	36.0	32.0	36.0
32	34.72404446784577	36.0	36.0	36.0	32.0	36.0
33	34.70806281589076	36.0	36.0	36.0	32.0	36.0
34	34.699651935432634	36.0	36.0	36.0	32.0	36.0
35	34.67164277337673	36.0	36.0	36.0	32.0	36.0
36	34.643803319314294	36.0	36.0	36.0	32.0	36.0
37	34.6393509321973	36.0	36.0	36.0	32.0	36.0
38	34.60847631594407	36.0	36.0	36.0	32.0	36.0
39	34.60328607981062	36.0	36.0	36.0	32.0	36.0
40	34.58876951913893	36.0	36.0	36.0	32.0	36.0
41	34.57329617526049	36.0	36.0	36.0	32.0	36.0
42	34.51903775567706	36.0	36.0	36.0	32.0	36.0
43	34.50965894132673	36.0	36.0	36.0	32.0	36.0
44	34.45908269565918	36.0	36.0	36.0	32.0	36.0
45	34.43780909156182	36.0	36.0	36.0	32.0	36.0
46	34.41717814770252	36.0	36.0	36.0	32.0	36.0
47	34.40287644170204	36.0	36.0	36.0	32.0	36.0
48	34.36087017773649	36.0	36.0	36.0	32.0	36.0
49	34.3500317114616	36.0	36.0	36.0	32.0	36.0
50	33.74643722000901	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	1.0
17	3.0
18	2.0
19	11.0
20	31.0
21	112.0
22	369.0
23	1062.0
24	2729.0
25	6082.0
26	12907.0
27	23536.0
28	39299.0
29	61000.0
30	88689.0
31	127795.0
32	185400.0
33	299181.0
34	630156.0
35	2198538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.73963364647042	17.712246073637353	11.736031364118286	26.81208891577394
2	16.155228176901964	19.905659248428833	36.68815769004407	27.250954884625134
3	18.106341928812743	23.62390648193829	28.767813268481497	29.501938320767472
4	12.288327353664931	16.03949953547876	35.59584367718058	36.076329433675724
5	14.352999153635777	36.635386727529465	33.67001150968314	15.341602609151611
6	34.95567868467423	35.54805285649674	16.370479580755415	13.125788878073616
7	30.602112701912453	30.245888999519433	20.623742317923536	18.52825598064458
8	28.284401301856484	33.23190739597972	18.813224063838508	19.670467238325298
9	27.250564068867533	13.863202692735316	18.001330484522647	40.884902753874506
10	15.491376107665253	27.064699998205	31.955289242667284	25.488634651462466
11	37.446442684629616	20.835067129882713	22.299805651605617	19.418684533882054
12	24.475454866871548	23.483287552013927	29.000870298349152	23.040387282765373
13	29.675523990163406	19.475006268898483	24.927479315354695	25.921990425583413
14	23.58241843119849	19.235327841744777	25.045173972829254	32.13707975422748
15	25.292350740382535	27.15470301472932	21.930765992121298	25.622180252766846
16	26.010878253474345	25.662620751251673	23.443074108815715	24.883426886458263
17	24.27925776686038	25.776468463685752	24.967173469853986	24.97710029959988
18	24.93465945404948	25.117177832352883	25.655839581820505	24.292323131777128
19	25.60807984547861	24.966017587632198	25.017936295283366	24.407966271605826
20	25.862579143186615	24.450974739453017	24.653456191118554	25.03298992624181
21	26.67409864385907	24.351492451257904	24.31809478844158	24.65631411644144
22	26.105598165411582	24.242371497084093	24.705206912016397	24.94682342548793
23	24.96195810182963	24.19176434720103	25.05812693252163	25.788150618447713
24	24.98736702742484	24.80425011817065	24.956525712246723	25.251857142157792
25	25.193910296073057	24.306297627656875	25.051178610699598	25.448613465570467
26	24.99908885933348	25.10146299272991	25.190945165650813	24.708502982285793
27	25.47371541716984	24.692603109326893	24.668532480389917	25.165148993113352
28	25.028204333186466	24.508504816767026	25.304481687581937	25.158809162464575
29	24.94618976776064	24.698012679469432	25.12297344709136	25.232824105678564
30	25.003522094000473	24.774844361521872	25.23320613905064	24.988427405427018
31	25.32255647339162	24.49226101092763	24.74115653872296	25.444025976957786
32	25.26235627690027	24.745571866649442	24.428718102141143	25.563353754309144
33	24.92914256589177	24.49960549387516	24.811647463560877	25.759604476672195
34	25.3651337860636	24.55271604162638	25.14165159242962	24.9404985798804
35	25.69263485869343	24.400530571071037	25.207293421196137	24.699541149039394
36	24.860082276544386	24.820562336381872	24.928949784109065	25.390405602964677
37	25.693577326409507	24.529294862258215	24.72569001441754	25.051437796914737
38	24.99180635304786	24.470105632088522	24.985169839001976	25.552918175861638
39	25.503198904895573	24.50491972887843	24.558228010945605	25.433653355280388
40	25.65664472184765	24.592920252893958	24.90338060453688	24.84705442072151
41	24.79964098972605	24.90310122321872	25.293062534422152	25.00419525263308
42	25.754373062868318	24.76090804356364	24.927709427800266	24.557009465767777
43	25.27649399226456	24.153302167103682	25.067238389020147	25.502965451611615
44	24.88912375195378	24.607795755439373	24.90095454067031	25.60212595193654
45	25.041829260465203	24.74388417048062	24.93983984125551	25.274446727798672
46	24.92991309985901	24.656201589180345	24.920829271901273	25.49305603905937
47	25.086127297328538	24.510250011898854	25.27139583744127	25.13222685333134
48	25.458798123929956	24.942049344069016	24.55582042707927	25.043332104921763
49	24.912310284384745	24.94064948998949	24.762128812840217	25.38491141278555
50	24.9152195262846	25.06466814383729	24.750268504363383	25.269843825514727
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	17.0
1	35.5
2	54.0
3	153.5
4	253.0
5	309.5
6	366.0
7	362.5
8	359.0
9	481.5
10	604.0
11	929.0
12	1254.0
13	2359.0
14	3464.0
15	4603.5
16	5743.0
17	5826.5
18	5910.0
19	6131.0
20	6352.0
21	7777.5
22	9203.0
23	10735.5
24	12268.0
25	16267.5
26	20267.0
27	25522.0
28	30777.0
29	36477.5
30	42178.0
31	47726.5
32	53275.0
33	62311.5
34	71348.0
35	83281.5
36	95215.0
37	111873.0
38	128531.0
39	141979.0
40	155427.0
41	171204.5
42	186982.0
43	195393.5
44	203805.0
45	221260.0
46	238715.0
47	255296.0
48	271877.0
49	281748.5
50	291620.0
51	288313.5
52	285007.0
53	285587.0
54	286167.0
55	280743.5
56	275320.0
57	264373.5
58	253427.0
59	233562.0
60	213697.0
61	188099.0
62	162501.0
63	140550.5
64	118600.0
65	100309.5
66	82019.0
67	69866.5
68	57714.0
69	49918.0
70	42122.0
71	32226.0
72	22330.0
73	19079.0
74	15828.0
75	12558.5
76	9289.0
77	8018.5
78	6748.0
79	5542.5
80	4337.0
81	3561.5
82	2786.0
83	2205.0
84	1624.0
85	1202.5
86	781.0
87	542.0
88	303.0
89	226.5
90	150.0
91	134.5
92	119.0
93	84.0
94	49.0
95	56.0
96	63.0
97	45.0
98	27.0
99	29.5
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009410090663231892
2	5.439358764873926E-4
3	2.719679382436963E-4
4	0.0
5	0.0
6	1.3598396912184815E-4
7	2.719679382436963E-5
8	2.719679382436963E-5
9	0.0013054461035697424
10	7.071166394336105E-4
11	5.439358764873926E-5
12	1.0878717529747852E-4
13	5.98329464136132E-4
14	2.1757435059495704E-4
15	0.004242699836601662
16	7.343134332579801E-4
17	0.0
18	3.8075511354117487E-4
19	5.439358764873926E-5
20	6.527230517848712E-4
21	0.0
22	8.15903814731089E-5
23	0.0010062813715016764
24	4.895422888386534E-4
25	0.0020397595368277224
26	0.005276178001927708
27	0.006092081816658798
28	0.00446027418719662
29	0.0025564986194907455
30	0.003100434495978138
31	0.007098363188160475
32	0.0035083864033436827
33	0.004215503042777293
34	0.002746876176261333
35	0.0030188441145050293
36	0.0075879054769991275
37	0.0036171735786411615
38	0.00747911830170165
39	0.004813832506913425
40	0.002882860145383181
41	0.0035083864033436827
42	0.002066956330652092
43	0.0016590044232865477
44	0.001550217247989069
45	0.001414233278867221
46	0.0010878717529747852
47	0.002148546712125201
48	0.0018765787738815045
49	5.16739082663023E-4
50	0.0014958236603403298
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3676904.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.17974059102392
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.00927286299424	30.04632032871248
2	15.567287323919569	13.75517431353403
3	6.374659751208394	8.448924424932837
4	3.254484060390751	5.751290461827425
5	1.8742075643230955	4.140100200276458
6	1.1747315795231101	3.1139601868448876
7	0.7498910742163196	2.3190995191280885
8	0.5237193912642034	1.8510229478833173
9	0.37839539665689215	1.5045669418625187
>10	1.9070829625159942	14.776277093163436
>50	0.1093237560027541	3.311812554682441
>100	0.06478606688202297	5.647969286156821
>500	0.008560372429134131	2.653449237444672
>1k	0.003597837673434894	2.6800325035506436
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.99164732068066E-4
2	0.0	0.0	0.0	0.0	2.99164732068066E-4
3	0.0	0.0	0.0	0.0	2.99164732068066E-4
4	0.0	0.0	0.0	0.0	2.99164732068066E-4
5	0.0	0.0	0.0	0.0	3.5355831971680524E-4
6	0.0	0.0	0.0	0.0	4.6234549501428376E-4
7	0.0	0.0	0.0	0.0	4.6234549501428376E-4
8	0.0	0.0	0.0	0.0	4.6234549501428376E-4
9	0.0	0.0	0.0	2.7196793824369633E-5	4.6234549501428376E-4
10	0.0	0.0	0.0	2.7196793824369633E-5	4.6234549501428376E-4
11	0.0	0.0	0.0	2.7196793824369633E-5	4.895422888386533E-4
12	0.0	0.0	0.0	2.7196793824369633E-5	9.246909900285675E-4
13	0.0	0.0	0.0	2.7196793824369633E-5	0.0010606749591504157
14	0.0	0.0	0.0	2.7196793824369633E-5	0.0010606749591504157
15	0.0	0.0	0.0	5.4393587648739266E-5	0.001196658928272264
16	0.0	0.0	0.0	1.3598396912184818E-4	0.0013054461035697424
17	0.0	0.0	0.0	1.3598396912184818E-4	0.0013054461035697424
18	0.0	0.0	0.0	1.9037755677058744E-4	0.0014686268665159601
19	0.0	0.0	0.0	2.7196793824369635E-4	0.0014686268665159601
20	0.0	0.0	0.0	3.263615258924356E-4	0.0017133980109352868
21	0.0	0.0	0.0	3.8075511354117487E-4	0.0017405948047596565
22	0.0	0.0	0.0	4.895422888386533E-4	0.0017949883924083957
23	0.0	0.0	0.0	8.15903814731089E-4	0.0018221851862327654
24	0.0	0.0	0.0	0.0011422653406235246	0.0018221851862327654
25	0.0	0.0	0.0	0.001414233278867221	0.0018221851862327654
26	0.0	0.0	0.0	0.0016590044232865477	0.0018765787738815047
27	0.0	0.0	0.0	0.002148546712125201	0.0019037755677058742
28	0.0	0.0	0.0	0.0034539928156949434	0.0019037755677058742
29	0.0	0.0	0.0	0.006336852961078125	0.0019037755677058742
30	0.0	0.0	0.0	0.013081657829521793	0.0019037755677058742
31	0.0	0.0	0.0	0.030460409083293987	0.0019037755677058742
32	0.0	0.0	0.0	0.048410293007377946	0.0019309723615302439
33	0.0	0.0	0.0	0.06742085189061232	0.0019309723615302439
34	0.0	0.0	0.0	0.08988540358954164	0.0020941531244764618
35	0.0	0.0	0.0	0.11547758657827346	0.0020941531244764618
36	2.7196793824369633E-5	0.0	0.0	0.1529819652620792	0.0020941531244764618
37	2.7196793824369633E-5	0.0	0.0	0.20990485473648482	0.0020941531244764618
38	2.7196793824369633E-5	0.0	0.0	0.29212076246755425	0.002148546712125201
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4070	0.0	27.40655	1
GGTATCA	1840	0.0	23.555328	1
TCAACGC	6285	0.0	17.571774	4
CAACGCA	6415	0.0	17.215683	5
TATCAAC	6465	0.0	17.149895	2
AACGCAG	6450	0.0	17.122263	6
ATCAACG	6455	0.0	17.109001	3
CGCAATA	830	0.0	16.699265	36
CGTCGTA	775	0.0	16.464085	10
TATAACG	125	9.455474E-6	15.839152	2
CTAGCGG	835	0.0	15.808398	29
TAGGACG	490	0.0	15.7140875	4
ACTAACG	85	0.0029817403	15.529215	3
ACCGTCG	825	0.0	15.199808	8
CAATACG	915	0.0	15.147761	38
GTATAGA	515	0.0	14.95208	1
TCTAGCG	900	0.0	14.911327	28
ACTATAC	370	0.0	14.8646755	3
CCGTCGT	815	0.0	14.847043	9
ATACCGT	845	0.0	14.840049	6
>>END_MODULE