FastQCFastQC Report
Thu 2 Feb 2017
SRR4063963_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063963_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051799
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31830.3026243607381258No Hit
TATCAACGCAGAGTACTTTTTTTTT22660.21544040258642572No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17810.1693289307177512No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11770.1119035100812988No Hit
GTCCTAAAGTGTGTATTTCTCATTT11630.11057245728508965No Hit
GGTATCAACGCAGAGTACTTTTTTT10990.1044876445024192No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10950.10410734370350228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGAC350.002166561516.28909915
AACCGCG653.369003E-614.6156417
CAACGGA400.00527365414.25160614
ACGAACG903.6243364E-813.72507415
CGAACGA903.626701E-813.724421516
CGATAAC852.6956332E-713.41210
ATAAGAT500.001502551513.2958063
ATTTGCG1301.4551915E-1113.15595416
TTGCGCG1301.4551915E-1113.15532918
GCGTTAT655.4580534E-513.1515741
AACGAAC957.366907E-813.00146514
CGAACGT604.094389E-412.6650814
TCGAACG751.4823059E-512.6626713
TCTTATG604.1026113E-412.662072
TTTGCGC1457.2759576E-1212.45027117
GATATAC2150.012.3699561
TAACGAA1001.4403486E-712.35021613
TGGATTG701.0905589E-412.2139195
GTGCTAT550.003071281112.088821
ATTCCGA951.0390759E-611.99963956
Produced by FastQC (version 0.11.5)