##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063963_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1051799
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.184804320977676	32.0	32.0	32.0	32.0	32.0
2	30.754795355386342	32.0	32.0	32.0	32.0	32.0
3	30.776240517437266	32.0	32.0	32.0	32.0	32.0
4	30.79930385938758	32.0	32.0	32.0	32.0	32.0
5	30.704839042440618	32.0	32.0	32.0	32.0	32.0
6	34.341837176114446	36.0	36.0	36.0	32.0	36.0
7	34.28106796070352	36.0	36.0	36.0	32.0	36.0
8	34.23901524911128	36.0	36.0	36.0	32.0	36.0
9	34.390815165254956	36.0	36.0	36.0	32.0	36.0
10	34.04221053642379	36.0	36.0	36.0	32.0	36.0
11	34.368299456455084	36.0	36.0	36.0	32.0	36.0
12	34.169348896509696	36.0	36.0	36.0	32.0	36.0
13	34.25995080809166	36.0	36.0	36.0	32.0	36.0
14	34.1562152084191	36.0	36.0	36.0	32.0	36.0
15	34.09291794344737	36.0	36.0	36.0	32.0	36.0
16	34.092250515545274	36.0	36.0	36.0	32.0	36.0
17	34.005819552975424	36.0	36.0	36.0	32.0	36.0
18	34.0057225762717	36.0	36.0	36.0	32.0	36.0
19	34.01585474030684	36.0	36.0	36.0	32.0	36.0
20	33.98480603233127	36.0	36.0	36.0	32.0	36.0
21	33.97020248165286	36.0	36.0	36.0	32.0	36.0
22	33.95737208344941	36.0	36.0	36.0	32.0	36.0
23	33.90991054374457	36.0	36.0	36.0	32.0	36.0
24	33.882659139246186	36.0	36.0	36.0	32.0	36.0
25	33.492596969573086	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	7.0
4	11.0
5	10.0
6	33.0
7	12.0
8	58.0
9	70.0
10	104.0
11	24.0
12	65.0
13	69.0
14	110.0
15	158.0
16	302.0
17	382.0
18	515.0
19	786.0
20	1227.0
21	1822.0
22	2907.0
23	4331.0
24	6514.0
25	9350.0
26	13177.0
27	17333.0
28	23090.0
29	30595.0
30	39616.0
31	53503.0
32	74097.0
33	103218.0
34	215413.0
35	452890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.51765791300874	17.105015360910052	11.508603060768332	25.868723665312878
2	16.195288180136117	19.729153078047958	38.439801547533705	25.635757194282228
3	18.81357092466281	24.193983901545764	28.628189598945784	28.364255574845643
4	12.470038117314616	15.648541054791382	36.08233111766736	35.79908971022664
5	14.348876547118843	36.95019458730661	33.6677401534449	15.033188712129638
6	33.60385905657363	35.70863476269807	17.260206759624822	13.427299421103477
7	30.023032315613097	30.40826728897663	20.909101283229013	18.65959911218126
8	27.904457465901107	33.505321815019016	19.39364311549922	19.196577603580657
9	27.750821605733883	13.970198659748215	18.33386442435282	39.94511531016508
10	16.005913247634368	26.81357193071911	31.811198450531254	25.369316371115268
11	37.03560658669533	20.79004195165571	23.03345668325073	19.140894778398227
12	25.177573507726088	23.313360872369696	29.511832208494987	21.99723341140923
13	30.067888972608475	19.751733375954213	25.03596136721693	25.144416284220377
14	23.602238055506135	19.890000009513926	25.457833812676544	31.0499281223034
15	24.849405040507474	27.9304656552165	22.45596310375926	24.76416620051676
16	25.0449761348209	25.625693907315387	24.756900542567735	24.572429415295975
17	23.442530310295226	26.055722994725667	25.815314595368022	24.686432099611082
18	24.224496048502655	24.953520191361704	27.201487353876423	23.620496406259218
19	25.70208255630742	24.505906665550288	25.515596337258422	24.27641444088387
20	25.988239324046585	24.4683908045977	25.195345208190606	24.348024663165106
21	26.604808001537588	24.05323321262267	24.592910738174574	24.74904804766517
22	25.90982789200052	24.715119775292873	25.04709943594857	24.32795289675804
23	24.32365823556895	24.705041625789434	25.734719686062853	25.23658045257876
24	24.969148107163825	24.896644969642878	25.694464927796577	24.439741995396716
25	24.87114517849378	24.667051700826555	25.792372707992183	24.66943041268748
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109.0
1	109.0
2	327.5
3	546.0
4	546.0
5	546.0
6	1398.5
7	2251.0
8	2251.0
9	2251.0
10	2224.0
11	2197.0
12	2197.0
13	2197.0
14	2313.5
15	2430.0
16	2430.0
17	2430.0
18	4283.5
19	6137.0
20	6137.0
21	6137.0
22	10482.0
23	14827.0
24	14827.0
25	14827.0
26	23311.5
27	31796.0
28	31796.0
29	31796.0
30	41615.0
31	51434.0
32	51434.0
33	51434.0
34	60439.5
35	69445.0
36	69445.0
37	69445.0
38	78484.5
39	87524.0
40	87524.0
41	87524.0
42	99649.0
43	111774.0
44	111774.0
45	111774.0
46	124557.0
47	137340.0
48	137340.0
49	137340.0
50	139759.5
51	142179.0
52	142179.0
53	142179.0
54	131248.0
55	120317.0
56	120317.0
57	120317.0
58	109598.5
59	98880.0
60	98880.0
61	98880.0
62	86984.0
63	75088.0
64	75088.0
65	75088.0
66	61720.5
67	48353.0
68	48353.0
69	48353.0
70	36074.5
71	23796.0
72	23796.0
73	23796.0
74	18011.5
75	12227.0
76	12227.0
77	12227.0
78	9810.5
79	7394.0
80	7394.0
81	7394.0
82	5060.0
83	2726.0
84	2726.0
85	2726.0
86	2134.5
87	1543.0
88	1543.0
89	1543.0
90	1098.5
91	654.0
92	654.0
93	654.0
94	461.5
95	269.0
96	269.0
97	269.0
98	416.0
99	563.0
100	563.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04078726068383788
2	0.005799587183482776
3	0.0019965791943137422
4	0.0043734591875443886
5	0.008176467176713421
6	0.013595753561279294
7	0.02215252153690962
8	0.03536797429927201
9	0.04791790066352982
10	0.057045119837535505
11	0.055999292640514016
12	0.06512651181451969
13	0.06379545901831053
14	0.0673132414082919
15	0.0603727518280584
16	0.06522158701424892
17	0.06379545901831053
18	0.07653553578202679
19	0.07444388138798383
20	0.07976809257282047
21	0.0761552349831099
22	0.07900749097498667
23	0.08442677735955254
24	0.07701091178067293
25	0.07701091178067293
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1051799.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.00282327605483
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.98392079714887	41.03346246630178
2	14.225047446216285	15.363854466630263
3	4.4429711103774965	7.197989510829991
4	1.907556618313496	4.1205377179141
5	0.9634893218894656	2.6015571789181355
6	0.56638480056597	1.835182697472458
7	0.36427161028414307	1.3770186776260946
8	0.2617588489841704	1.1308573490108544
9	0.1776264463124027	0.8633096630426094
>10	0.9373484203017994	9.133694789429178
>50	0.08252066001672441	3.1126975820208638
>100	0.07878301737598305	8.451168227625255
>500	0.007081618904953475	2.6539506479204875
>1k	0.001239283308366858	1.1247190252579924
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3183	0.3026243607381258	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2266	0.21544040258642572	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1781	0.1693289307177512	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1177	0.1119035100812988	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1163	0.11057245728508965	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1099	0.1044876445024192	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1095	0.10410734370350228	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9015039945845166E-4
2	0.0	0.0	0.0	9.507519972922583E-5	1.9015039945845166E-4
3	0.0	0.0	0.0	9.507519972922583E-5	1.9015039945845166E-4
4	0.0	0.0	0.0	9.507519972922583E-5	1.9015039945845166E-4
5	0.0	0.0	0.0	9.507519972922583E-5	1.9015039945845166E-4
6	0.0	0.0	0.0	9.507519972922583E-5	1.9015039945845166E-4
7	0.0	0.0	0.0	9.507519972922583E-5	1.9015039945845166E-4
8	0.0	0.0	0.0	9.507519972922583E-5	2.852255991876775E-4
9	0.0	0.0	0.0	9.507519972922583E-5	2.852255991876775E-4
10	0.0	0.0	0.0	9.507519972922583E-5	2.852255991876775E-4
11	0.0	0.0	0.0	9.507519972922583E-5	2.852255991876775E-4
12	0.0	0.0	0.0	9.507519972922583E-5	5.70451198375355E-4
13	0.0	0.0	0.0	9.507519972922583E-5	5.70451198375355E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGAC	35	0.0021665615	16.289099	15
AACCGCG	65	3.369003E-6	14.615641	7
CAACGGA	40	0.005273654	14.251606	14
ACGAACG	90	3.6243364E-8	13.725074	15
CGAACGA	90	3.626701E-8	13.7244215	16
CGATAAC	85	2.6956332E-7	13.412	10
ATAAGAT	50	0.0015025515	13.295806	3
ATTTGCG	130	1.4551915E-11	13.155954	16
TTGCGCG	130	1.4551915E-11	13.155329	18
GCGTTAT	65	5.4580534E-5	13.151574	1
AACGAAC	95	7.366907E-8	13.001465	14
CGAACGT	60	4.094389E-4	12.665081	4
TCGAACG	75	1.4823059E-5	12.662671	3
TCTTATG	60	4.1026113E-4	12.66207	2
TTTGCGC	145	7.2759576E-12	12.450271	17
GATATAC	215	0.0	12.369956	1
TAACGAA	100	1.4403486E-7	12.350216	13
TGGATTG	70	1.0905589E-4	12.213919	5
GTGCTAT	55	0.0030712811	12.08882	1
ATTCCGA	95	1.0390759E-6	11.9996395	6
>>END_MODULE