FastQCFastQC Report
Thu 2 Feb 2017
SRR4063963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051799
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACG6850.022.481634
AGGACGT6750.022.1628445
AATCGTA703.2126154E-522.00197426
GGACGTG6900.021.0433646
GATATAC3450.021.0423641
ATACACT4400.019.4998934
CTATAGA1252.2884706E-819.3589761
ACCGTCG2450.018.8570428
GACGTGA4450.018.7864157
GTATCAA26850.018.7625081
GTAGGAC6350.018.708563
TTAGGAC5550.018.630533
GTATAGG1303.5881385E-818.6143991
TGTAGGA6600.017.9990482
CGTCGTA2450.017.95823310
CGAGCCG2700.017.92497615
ATACCGT2350.017.7871386
CCGTCGT2600.017.7691359
ACTGTTC4250.017.5999058
GTCCTAA4250.017.599071
Produced by FastQC (version 0.11.5)