FastQCFastQC Report
Thu 2 Feb 2017
SRR4063962_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063962_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310199
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31870.24324549171538065No Hit
TATCAACGCAGAGTACTTTTTTTTT21350.16295234540707176No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14870.11349420965822749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGG456.7702937E-414.7751936
TAGATCG456.772255E-414.7746295
ACGCGCG400.005271265314.25295213
GTAATAC1301.4551915E-1113.1475293
ATTCCGA1102.752131E-912.9522786
TAATACT1800.012.6639684
GGATATA604.10523E-412.661551
AATTCCG1001.4457328E-712.3473685
CGGTCCA1551.8189894E-1212.26013510
AGCTCGC550.003064121812.09295110
CGCCAGT1501.4551915E-1112.03628618
CCCTTAA951.0444292E-611.9951521
GTCTATA802.877427E-511.8702021
CGTCGTA1459.640644E-1111.79509510
CCGTCGT1459.640644E-1111.7941949
GTCCTAA2750.011.7407081
GAATAAA1157.108429E-811.5605451
ACGAACG1001.9229374E-611.40279815
CGAACGA1001.9238214E-611.40236316
TTATACT1251.835906E-811.3975714
Produced by FastQC (version 0.11.5)