##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063962_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1310199
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260496306286296	32.0	32.0	32.0	32.0	32.0
2	30.853673373281463	32.0	32.0	32.0	32.0	32.0
3	30.88014187157829	32.0	32.0	32.0	32.0	32.0
4	30.907241571700176	32.0	32.0	32.0	32.0	32.0
5	30.807441464998828	32.0	32.0	32.0	32.0	32.0
6	34.48051708175628	36.0	36.0	36.0	32.0	36.0
7	34.424549247862345	36.0	36.0	36.0	32.0	36.0
8	34.37220681743766	36.0	36.0	36.0	32.0	36.0
9	34.514927885000674	36.0	36.0	36.0	32.0	36.0
10	34.2067678268721	36.0	36.0	36.0	32.0	36.0
11	34.493738737397905	36.0	36.0	36.0	32.0	36.0
12	34.311761037827075	36.0	36.0	36.0	32.0	36.0
13	34.39419355380366	36.0	36.0	36.0	32.0	36.0
14	34.293398941687485	36.0	36.0	36.0	32.0	36.0
15	34.22831798833612	36.0	36.0	36.0	32.0	36.0
16	34.237391419166094	36.0	36.0	36.0	32.0	36.0
17	34.15457575528603	36.0	36.0	36.0	32.0	36.0
18	34.1536026206706	36.0	36.0	36.0	32.0	36.0
19	34.165799241183976	36.0	36.0	36.0	32.0	36.0
20	34.14756536984076	36.0	36.0	36.0	32.0	36.0
21	34.13658459516455	36.0	36.0	36.0	32.0	36.0
22	34.11128156867773	36.0	36.0	36.0	32.0	36.0
23	34.0521218532452	36.0	36.0	36.0	32.0	36.0
24	34.041598261027524	36.0	36.0	36.0	32.0	36.0
25	33.63995240417677	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	10.0
5	15.0
6	51.0
7	19.0
8	78.0
9	72.0
10	122.0
11	37.0
12	86.0
13	69.0
14	142.0
15	217.0
16	398.0
17	507.0
18	628.0
19	857.0
20	1315.0
21	1847.0
22	2945.0
23	4570.0
24	6731.0
25	10034.0
26	14159.0
27	18874.0
28	25967.0
29	35020.0
30	46280.0
31	62795.0
32	88838.0
33	126133.0
34	268612.0
35	592767.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.698543784206414	17.02417965695856	11.365538262314308	25.91173829652072
2	16.442759127084987	19.707145577682976	38.06977732897044	25.780317966261595
3	18.530173670528217	24.245333854380537	28.635716259267795	28.58877621582345
4	12.427136595079826	15.720100720753313	36.25741037142877	35.59535231273809
5	14.369580483634586	36.89247984855887	33.87945774303859	14.858481924767952
6	33.76696241075579	35.766154795046525	17.242977063866523	13.223905730331161
7	30.13197079801605	30.47107780604942	21.043032694587648	18.353918701346885
8	28.113509204852242	33.09349002553171	19.428987649494864	19.364013120121186
9	27.83495550420675	13.885787767207662	18.28542673046408	39.99382999812151
10	15.664460415392286	26.88098385518927	32.11458760512914	25.339968124289307
11	36.94879711518415	20.90827101201472	22.999793047147833	19.143138825653295
12	24.897298643097223	23.469828063532407	29.394697791854004	22.23817550151637
13	29.7863971784387	19.679672141664618	25.13980261901743	25.39412806087925
14	23.526801534833137	19.707055833221315	25.52782801559273	31.238314616352813
15	24.943944476010678	27.621168520928414	22.377915039987293	25.056971963073615
16	25.41641621993589	25.658754459352018	24.308366053705452	24.616463267006637
17	23.957891006626117	25.8807329359903	25.446094439743266	24.71528161764032
18	24.56994236872558	24.941165687049576	26.53650935505677	23.95238258916807
19	25.5965488348575	24.740631771586393	25.449898757980783	24.212920635575323
20	25.974293813145486	24.33899413296425	25.09901243451048	24.58769961937978
21	26.76038694943153	24.177312358932642	24.45534155963688	24.606959131998945
22	25.991298449730827	24.47998460118913	24.884661684458813	24.64405526462123
23	24.433242635859063	24.429957971574666	25.447134474155803	25.689664918410465
24	24.83868060287014	24.81973779487383	25.501984411902196	24.83959719035383
25	24.918862974094967	24.538097547748443	25.417868095523623	25.12517138263297
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	177.0
1	177.0
2	349.0
3	521.0
4	521.0
5	521.0
6	1314.0
7	2107.0
8	2107.0
9	2107.0
10	2205.5
11	2304.0
12	2304.0
13	2304.0
14	2773.0
15	3242.0
16	3242.0
17	3242.0
18	5676.5
19	8111.0
20	8111.0
21	8111.0
22	13445.5
23	18780.0
24	18780.0
25	18780.0
26	28353.0
27	37926.0
28	37926.0
29	37926.0
30	48586.5
31	59247.0
32	59247.0
33	59247.0
34	71611.0
35	83975.0
36	83975.0
37	83975.0
38	96945.5
39	109916.0
40	109916.0
41	109916.0
42	125149.5
43	140383.0
44	140383.0
45	140383.0
46	155648.0
47	170913.0
48	170913.0
49	170913.0
50	174550.0
51	178187.0
52	178187.0
53	178187.0
54	165907.0
55	153627.0
56	153627.0
57	153627.0
58	139753.5
59	125880.0
60	125880.0
61	125880.0
62	110088.0
63	94296.0
64	94296.0
65	94296.0
66	76990.5
67	59685.0
68	59685.0
69	59685.0
70	44743.0
71	29801.0
72	29801.0
73	29801.0
74	22627.5
75	15454.0
76	15454.0
77	15454.0
78	12201.5
79	8949.0
80	8949.0
81	8949.0
82	6130.5
83	3312.0
84	3312.0
85	3312.0
86	2506.5
87	1701.0
88	1701.0
89	1701.0
90	1211.5
91	722.0
92	722.0
93	722.0
94	510.5
95	299.0
96	299.0
97	299.0
98	491.5
99	684.0
100	684.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03839111463220473
2	0.004579457013781876
3	0.0012975128205715315
4	0.0032056199096473135
5	0.00908258974400072
6	0.012975128205715315
7	0.021981393666153003
8	0.03472754902117922
9	0.048618568629650916
10	0.05632732126951707
11	0.05510613273250857
12	0.06258591252168565
13	0.06510461387926567
14	0.06647845098340023
15	0.05976191402985348
16	0.06655477526696327
17	0.0637307767751311
18	0.0773928235329137
19	0.07327131222051002
20	0.07785076923429189
21	0.07510309502602276
22	0.07685855354797248
23	0.08281184766588892
24	0.07594266214521611
25	0.07586633786165307
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1310199.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.593600624821924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.22409375681922	43.7038952068723
2	13.807948715240888	15.628830700767319
3	4.162191535762888	7.066602164969374
4	1.7339731044621967	3.925271254724648
5	0.8753126615627346	2.476854759516566
6	0.5088415161136477	1.7278304126558803
7	0.3277081399017478	1.2982328513771952
8	0.2260583317110875	1.0234763954216644
9	0.16095904152966148	0.819832654195541
>10	0.8332276287852846	8.408714821169607
>50	0.06650551050154632	2.6549958868937926
>100	0.06545551484679725	7.660812831633885
>500	0.0063693598216141905	2.495184788204361
>1k	0.0013551829407689767	1.1094652715978446
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3187	0.24324549171538065	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2135	0.16295234540707176	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1487	0.11349420965822749	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5264856712606252E-4
2	0.0	0.0	0.0	0.0	1.5264856712606252E-4
3	0.0	0.0	0.0	0.0	1.5264856712606252E-4
4	0.0	0.0	0.0	0.0	1.5264856712606252E-4
5	0.0	0.0	0.0	0.0	1.5264856712606252E-4
6	0.0	0.0	0.0	0.0	2.289728506890938E-4
7	0.0	0.0	0.0	0.0	2.289728506890938E-4
8	0.0	0.0	0.0	0.0	2.289728506890938E-4
9	0.0	0.0	0.0	0.0	2.289728506890938E-4
10	0.0	0.0	0.0	0.0	3.0529713425212504E-4
11	0.0	0.0	0.0	0.0	3.0529713425212504E-4
12	0.0	0.0	0.0	0.0	6.105942685042501E-4
13	0.0	0.0	0.0	0.0	7.632428356303127E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGATCGG	45	6.7702937E-4	14.775193	6
TAGATCG	45	6.772255E-4	14.774629	5
ACGCGCG	40	0.0052712653	14.252952	13
GTAATAC	130	1.4551915E-11	13.147529	3
ATTCCGA	110	2.752131E-9	12.952278	6
TAATACT	180	0.0	12.663968	4
GGATATA	60	4.10523E-4	12.66155	1
AATTCCG	100	1.4457328E-7	12.347368	5
CGGTCCA	155	1.8189894E-12	12.260135	10
AGCTCGC	55	0.0030641218	12.092951	10
CGCCAGT	150	1.4551915E-11	12.036286	18
CCCTTAA	95	1.0444292E-6	11.995152	1
GTCTATA	80	2.877427E-5	11.870202	1
CGTCGTA	145	9.640644E-11	11.795095	10
CCGTCGT	145	9.640644E-11	11.794194	9
GTCCTAA	275	0.0	11.740708	1
GAATAAA	115	7.108429E-8	11.560545	1
ACGAACG	100	1.9229374E-6	11.402798	15
CGAACGA	100	1.9238214E-6	11.402363	16
TTATACT	125	1.835906E-8	11.397571	4
>>END_MODULE