FastQCFastQC Report
Thu 2 Feb 2017
SRR4063962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1310199
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTTCG2550.024.15707241
GTATCAA24800.021.645351
CGGATAT550.004486555219.99788317
ATTTCGT2800.019.64377842
GTATAGA1800.019.5557251
GTATAAT1901.8189894E-1218.5264761
ATAGTAC851.432109E-418.1157283
GTATTAC1951.8189894E-1218.051441
CCTAATA1102.7996157E-617.9980952
GTGTAAT2700.017.926081
TAGGACG4350.017.6992764
AATCACG3000.017.60216734
GGGTATA750.001290873417.6001531
TATTAGG1254.85863E-717.5981372
AACGCAG30200.017.5544346
CGGTCCG1903.274181E-1117.36790839
TGTAATA2450.017.059422
GTATTAG2201.8189894E-1217.0001491
ACCGTCG2750.016.7982228
TCACGGA3150.016.7639736