##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063962_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1310199
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332141911266916	32.0	32.0	32.0	32.0	32.0
2	31.422027493533424	32.0	32.0	32.0	32.0	32.0
3	31.497126009102434	32.0	32.0	32.0	32.0	32.0
4	31.582522960252604	32.0	32.0	32.0	32.0	32.0
5	31.534902713251956	32.0	32.0	32.0	32.0	32.0
6	35.13260046756256	36.0	36.0	36.0	36.0	36.0
7	35.144535295783314	36.0	36.0	36.0	36.0	36.0
8	35.096001447108414	36.0	36.0	36.0	36.0	36.0
9	35.2106557858768	36.0	36.0	36.0	36.0	36.0
10	35.06067475246127	36.0	36.0	36.0	36.0	36.0
11	35.19984216138159	36.0	36.0	36.0	36.0	36.0
12	35.12065800691345	36.0	36.0	36.0	36.0	36.0
13	35.14986196753318	36.0	36.0	36.0	36.0	36.0
14	35.09605182113557	36.0	36.0	36.0	36.0	36.0
15	35.07280497084794	36.0	36.0	36.0	36.0	36.0
16	35.08421239826927	36.0	36.0	36.0	36.0	36.0
17	35.05961995086243	36.0	36.0	36.0	36.0	36.0
18	35.04272480745291	36.0	36.0	36.0	36.0	36.0
19	35.04490386574864	36.0	36.0	36.0	36.0	36.0
20	35.03902765915712	36.0	36.0	36.0	36.0	36.0
21	35.03618915905141	36.0	36.0	36.0	36.0	36.0
22	35.01873303215771	36.0	36.0	36.0	36.0	36.0
23	34.965734212894375	36.0	36.0	36.0	36.0	36.0
24	34.93811703412993	36.0	36.0	36.0	36.0	36.0
25	34.913181890689884	36.0	36.0	36.0	32.0	36.0
26	34.84983731478958	36.0	36.0	36.0	32.0	36.0
27	34.84466481809252	36.0	36.0	36.0	32.0	36.0
28	34.823057413415825	36.0	36.0	36.0	32.0	36.0
29	34.79889009226843	36.0	36.0	36.0	32.0	36.0
30	34.77552035988426	36.0	36.0	36.0	32.0	36.0
31	34.77392136614362	36.0	36.0	36.0	32.0	36.0
32	34.732748994618376	36.0	36.0	36.0	32.0	36.0
33	34.717381863365794	36.0	36.0	36.0	32.0	36.0
34	34.70766349233971	36.0	36.0	36.0	32.0	36.0
35	34.66620719447962	36.0	36.0	36.0	32.0	36.0
36	34.637020025202276	36.0	36.0	36.0	32.0	36.0
37	34.62509282940989	36.0	36.0	36.0	32.0	36.0
38	34.58013782639126	36.0	36.0	36.0	32.0	36.0
39	34.57549502022212	36.0	36.0	36.0	32.0	36.0
40	34.56087434046278	36.0	36.0	36.0	32.0	36.0
41	34.54778319934606	36.0	36.0	36.0	32.0	36.0
42	34.48258775956935	36.0	36.0	36.0	32.0	36.0
43	34.47123833860353	36.0	36.0	36.0	32.0	36.0
44	34.40855015154187	36.0	36.0	36.0	32.0	36.0
45	34.37125734335013	36.0	36.0	36.0	32.0	36.0
46	34.352714358658496	36.0	36.0	36.0	32.0	36.0
47	34.31612526036121	36.0	36.0	36.0	32.0	36.0
48	34.274000361777105	36.0	36.0	36.0	32.0	36.0
49	34.24590462975472	36.0	36.0	36.0	32.0	36.0
50	33.671028599472294	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	4.0
20	7.0
21	37.0
22	139.0
23	372.0
24	1034.0
25	2243.0
26	4675.0
27	8697.0
28	14137.0
29	21615.0
30	31531.0
31	45021.0
32	65905.0
33	106286.0
34	222232.0
35	786263.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.69076697606253	17.782730825598435	11.924844284318514	26.601657914020514
2	15.700587623350149	20.514000609070575	37.51462570781557	26.27078605976371
3	17.892756421754015	24.741049996374585	28.893943267986828	28.472250313884572
4	12.096635701904825	16.27157401280264	36.31746017208073	35.31433011321181
5	13.938416988564335	37.439656113307976	33.63763825189914	14.98428864622855
6	33.9354051830334	35.98364522003544	16.9623217254186	13.118627871512551
7	29.962517087125068	30.701642577413928	21.014091773985133	18.32174856147587
8	28.0000213707994	33.35241440422409	19.3480532346613	19.29951099031521
9	27.789150226227765	13.75753329303365	18.17996556208899	40.27335091864959
10	15.44261036351133	27.159433762812046	32.23395331832791	25.164002555348713
11	37.10672958840603	20.741582003955124	22.955596821551534	19.196091586087306
12	24.749923294036474	23.617575358838895	29.324575369524304	22.30792597760033
13	29.8652718605031	19.622590242361053	25.114601349113187	25.397536548022657
14	23.532568003132354	19.58064321624916	25.63156532948862	31.255223451129872
15	25.009102087641082	27.68752017915656	22.300076556762296	25.003301176440058
16	25.43473133782687	25.611730323460115	24.275193351737972	24.67834498697504
17	23.954452720540925	25.89240260448985	25.44926381412289	24.70388086084633
18	24.593743679375972	24.934227347837535	26.556657596769945	23.915371376016548
19	25.635133010430483	24.77885021958513	25.522249308883083	24.0637674611013
20	25.99279493813874	24.219922301345605	25.17505094680924	24.61223181370641
21	26.740518043442258	24.150835102148605	24.544439432483156	24.564207421925982
22	25.949035222893624	24.48811211121364	24.93430387292312	24.628548792969617
23	24.378888268962314	24.391329203146718	25.61550186194227	25.61428066594871
24	24.873777945353005	24.795926700763477	25.501701660292277	24.828593693591237
25	24.955826376862255	24.439481563856933	25.543154290546806	25.061537768734006
26	24.502913442441766	25.2586376660708	25.826747493004532	24.411701398482904
27	25.195707263456786	24.924205416297742	25.052361615729858	24.827725704515615
28	24.5260106430074	24.90046986771041	25.52741810898855	25.046101380293646
29	24.57963342049786	24.883106415522917	25.346635464513657	25.190624699465562
30	24.57275753999936	24.969125578370654	25.66683407803405	24.791282803595934
31	25.272404325494453	24.558638203547382	24.882889993305817	25.286067477652352
32	24.835745252488245	24.960462844232765	24.926421200464066	25.27737070281492
33	24.49946456390008	24.68173418707977	25.441038295702693	25.37776295331745
34	25.139639646515917	24.785789194891	25.465172902810195	24.609398255782892
35	25.576398625817742	24.61575333107916	25.476487240881085	24.331360802222015
36	24.62458542766309	25.176381685157835	25.239202036509084	24.959830850669995
37	25.302505146384785	25.037648562027197	24.91331098971639	24.746535301871628
38	24.730434251604876	25.020800415244988	25.27376399734365	24.975001335806486
39	25.475692348087193	24.641355943587836	24.809505527259237	25.073446181065727
40	25.611315198749473	24.881789878694853	25.20396254828583	24.302932374269844
41	24.674675400506658	25.143322955410554	25.537475394095193	24.644526249987596
42	25.617611163140026	25.323908594998052	25.054630356847095	24.003849885014834
43	24.87932955777069	24.68363125677388	25.42704055931246	25.00999862614297
44	24.66105879865637	25.120536596796022	24.9346083715023	25.283796233045308
45	24.768467281644842	25.245881869847093	25.02011170967087	24.9655391388372
46	24.595516680786755	24.94897686595074	25.058121341179522	25.397385112082983
47	24.930581315650077	24.8067802095307	25.410749477547117	24.851888997272102
48	25.242221214716302	25.314654784202915	24.600394148527744	24.842729852553035
49	24.78896222843675	25.31712909252995	24.902533369155055	24.991375309878247
50	24.786078195660885	25.57826244585647	24.71448470624153	24.921174652241113
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	45.5
2	28.0
3	110.0
4	192.0
5	214.5
6	237.0
7	215.0
8	193.0
9	263.5
10	334.0
11	525.0
12	716.0
13	1288.0
14	1860.0
15	2475.5
16	3091.0
17	3299.5
18	3508.0
19	3616.0
20	3724.0
21	4085.5
22	4447.0
23	5155.0
24	5863.0
25	6777.0
26	7691.0
27	9742.0
28	11793.0
29	14194.5
30	16596.0
31	19362.0
32	22128.0
33	26012.0
34	29896.0
35	34888.5
36	39881.0
37	45078.0
38	50275.0
39	53951.5
40	57628.0
41	62247.5
42	66867.0
43	69425.0
44	71983.0
45	78609.5
46	85236.0
47	90384.5
48	95533.0
49	99007.5
50	102482.0
51	99809.5
52	97137.0
53	96501.0
54	95865.0
55	94742.5
56	93620.0
57	90795.0
58	87970.0
59	79603.0
60	71236.0
61	62996.0
62	54756.0
63	47783.0
64	40810.0
65	34391.0
66	27972.0
67	23907.5
68	19843.0
69	17586.0
70	15329.0
71	11798.0
72	8267.0
73	7116.5
74	5966.0
75	4657.0
76	3348.0
77	2863.5
78	2379.0
79	1954.5
80	1530.0
81	1193.0
82	856.0
83	710.0
84	564.0
85	407.0
86	250.0
87	175.0
88	100.0
89	77.5
90	55.0
91	45.0
92	35.0
93	27.5
94	20.0
95	21.0
96	22.0
97	15.0
98	8.0
99	12.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00908258974400072
2	4.5794570137818754E-4
3	3.052971342521251E-4
4	0.0
5	0.0
6	7.632428356303127E-5
7	1.5264856712606255E-4
8	0.0
9	0.001144864253445469
10	4.5794570137818754E-4
11	0.0
12	7.632428356303127E-5
13	3.8162141781515634E-4
14	1.5264856712606255E-4
15	0.004579457013781876
16	0.0016791342383866879
17	0.0
18	3.052971342521251E-4
19	7.632428356303127E-5
20	6.869185520672813E-4
21	0.0
22	0.0
23	6.105942685042502E-4
24	6.105942685042502E-4
25	0.0021370799397648754
26	0.004961078431597032
27	0.00602961840147947
28	0.0032819441932103446
29	0.0025187013575800315
30	0.004045187028840657
31	0.008090374057681314
32	0.002976647058958219
33	0.003968862745277625
34	0.003129295626084282
35	0.002900322775395188
36	0.008700968326185565
37	0.004274159879529751
38	0.009845832579631033
39	0.004274159879529751
40	0.0024423770740170008
41	0.0035109170438994385
42	0.002289728506890938
43	0.001450161387697594
44	0.0012211885370085004
45	0.0012975128205715315
46	6.869185520672813E-4
47	0.0025187013575800315
48	0.0025187013575800315
49	5.342699849412188E-4
50	0.0018317828055127501
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1310199.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.97768233016194
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.15920478030733	46.878079554913484
2	13.454978729484314	16.13996879992192
3	4.016697113340372	7.227365504412221
4	1.6101564559713417	3.8629380967243403
5	0.8194841123815583	2.4575378883517915
6	0.4598061062763984	1.65468627454287
7	0.28724382752236227	1.2059753326905251
8	0.21727206842731048	1.042518007948035
9	0.1484995432819186	0.8015992589823344
>10	0.7150666443603173	7.644182193049816
>50	0.05284113997165359	2.216459699960229
>100	0.05286176236410674	6.3485582013400075
>500	0.005375740979732098	2.1879572001132197
>1k	5.119753314030569E-4	0.3321739870492229
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	7.632428356303126E-5
15	0.0	0.0	0.0	7.632428356303126E-5	2.289728506890938E-4
16	0.0	0.0	0.0	7.632428356303126E-5	3.0529713425212504E-4
17	0.0	0.0	0.0	7.632428356303126E-5	3.0529713425212504E-4
18	0.0	0.0	0.0	7.632428356303126E-5	3.0529713425212504E-4
19	0.0	0.0	0.0	7.632428356303126E-5	3.0529713425212504E-4
20	0.0	0.0	0.0	2.289728506890938E-4	3.0529713425212504E-4
21	0.0	0.0	0.0	3.0529713425212504E-4	3.0529713425212504E-4
22	0.0	0.0	0.0	3.0529713425212504E-4	3.0529713425212504E-4
23	0.0	0.0	0.0	0.0010685399698824377	3.0529713425212504E-4
24	0.0	0.0	0.0	0.002060755656201844	3.0529713425212504E-4
25	0.0	0.0	0.0	0.0022134042233279065	3.0529713425212504E-4
26	0.0	0.0	0.0	0.002823998491832157	3.0529713425212504E-4
27	0.0	0.0	0.0	0.0033582684767733757	3.0529713425212504E-4
28	0.0	0.0	0.0	0.005037402715160064	3.0529713425212504E-4
29	0.0	0.0	0.0	0.008395671191933438	3.0529713425212504E-4
30	0.0	0.0	0.0	0.015875450981110505	3.0529713425212504E-4
31	0.0	0.0	0.0	0.036177710408876816	3.0529713425212504E-4
32	0.0	0.0	0.0	0.05617467270239101	3.0529713425212504E-4
33	0.0	0.0	0.0	0.07510309502602276	3.0529713425212504E-4
34	0.0	0.0	0.0	0.09807670437849518	5.342699849412188E-4
35	0.0	0.0	0.0	0.12509550075980824	5.342699849412188E-4
36	0.0	0.0	0.0	0.1650131010632736	5.342699849412188E-4
37	0.0	0.0	0.0	0.2211877737656646	5.342699849412188E-4
38	0.0	0.0	0.0	0.29957281298489774	5.342699849412188E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTTCG	255	0.0	24.157072	41
GTATCAA	2480	0.0	21.64535	1
CGGATAT	55	0.0044865552	19.997883	17
ATTTCGT	280	0.0	19.643778	42
GTATAGA	180	0.0	19.555725	1
GTATAAT	190	1.8189894E-12	18.526476	1
ATAGTAC	85	1.432109E-4	18.115728	3
GTATTAC	195	1.8189894E-12	18.05144	1
CCTAATA	110	2.7996157E-6	17.998095	2
GTGTAAT	270	0.0	17.92608	1
TAGGACG	435	0.0	17.699276	4
AATCACG	300	0.0	17.602167	34
GGGTATA	75	0.0012908734	17.600153	1
TATTAGG	125	4.85863E-7	17.598137	2
AACGCAG	3020	0.0	17.554434	6
CGGTCCG	190	3.274181E-11	17.367908	39
TGTAATA	245	0.0	17.05942	2
GTATTAG	220	1.8189894E-12	17.000149	1
ACCGTCG	275	0.0	16.798222	8
TCACGGA	315	0.0	16.76397	36
>>END_MODULE