FastQCFastQC Report
Thu 2 Feb 2017
SRR4063961_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063961_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2578507
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60600.23501972265345797No Hit
TATCAACGCAGAGTACTTTTTTTTT42160.16350547041369287No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG31390.12173711376389515No Hit
CTATTGGAGCTGGAATTACCGCGGC29130.1129723518299543No Hit
GTATCTGATCGTCTTCGAACCTCCG28530.11064542388289036No Hit
GAATAGGACCGCGGTTCTATTTTGT28470.11041273108818397No Hit
GATTAAGAGGGACGGCCGGGGGCAT27120.1051771432072901No Hit
GCGCAAGACGGACCAGAGCGAAAGC26870.10420758989601346No Hit
GAACTACGACGGTATCTGATCGTCT26740.10370342217414961No Hit
GTCTTGCGCCGGTCCAAGAATTTCA26630.1032768187171879No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGT707.2745006E-613.5700696
CGAACGA2000.013.30176616
GTATCAA40550.012.040251
GTCTAAA1459.822543E-1111.7914661
CGACCAT4850.011.752542510
CGTCGTA5250.011.58091610
ACGAACG2300.011.56675315
CCGTCGT5300.011.4714419
AGGCCCG6300.011.46028310
CGAGCCG6150.011.43241315
CGCATCG2750.011.40107113
GTATTAG4250.011.39841651
ACCGTCG5550.011.1258768
TCGAACG2150.011.044123
CGCTTAC951.3584593E-511.00188818
CGACGCT951.3590439E-511.00146215
CCGACCA5400.010.9071629
GTTCAAA7950.010.8727041
GTACGCC1408.378265E-910.8556354
TCCATTA5700.010.833098
Produced by FastQC (version 0.11.5)