##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063961_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2578507
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23780311629947	32.0	32.0	32.0	32.0	32.0
2	30.842810199855965	32.0	32.0	32.0	32.0	32.0
3	30.86636918185601	32.0	32.0	32.0	32.0	32.0
4	30.885999146017443	32.0	32.0	32.0	32.0	32.0
5	30.803744182195356	32.0	32.0	32.0	32.0	32.0
6	34.452978797420364	36.0	36.0	36.0	32.0	36.0
7	34.38681221342428	36.0	36.0	36.0	32.0	36.0
8	34.35023096698981	36.0	36.0	36.0	32.0	36.0
9	34.47941114761372	36.0	36.0	36.0	32.0	36.0
10	34.18785754702237	36.0	36.0	36.0	32.0	36.0
11	34.45382502355045	36.0	36.0	36.0	32.0	36.0
12	34.270394844768695	36.0	36.0	36.0	32.0	36.0
13	34.35484836767944	36.0	36.0	36.0	32.0	36.0
14	34.25686531004182	36.0	36.0	36.0	32.0	36.0
15	34.192398546911065	36.0	36.0	36.0	32.0	36.0
16	34.191217630977924	36.0	36.0	36.0	32.0	36.0
17	34.11708442133374	36.0	36.0	36.0	32.0	36.0
18	34.106852531329174	36.0	36.0	36.0	32.0	36.0
19	34.12521548322343	36.0	36.0	36.0	32.0	36.0
20	34.104544218805685	36.0	36.0	36.0	32.0	36.0
21	34.095896191090425	36.0	36.0	36.0	32.0	36.0
22	34.06822087355202	36.0	36.0	36.0	32.0	36.0
23	34.01001858827608	36.0	36.0	36.0	32.0	36.0
24	33.994539863572214	36.0	36.0	36.0	32.0	36.0
25	33.60681277964341	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2.0
3	7.0
4	22.0
5	27.0
6	119.0
7	34.0
8	139.0
9	167.0
10	240.0
11	72.0
12	142.0
13	150.0
14	259.0
15	442.0
16	786.0
17	1024.0
18	1331.0
19	1892.0
20	2866.0
21	4301.0
22	6540.0
23	10051.0
24	14486.0
25	21136.0
26	29589.0
27	38928.0
28	52181.0
29	69774.0
30	92072.0
31	123691.0
32	172108.0
33	240774.0
34	518355.0
35	1174800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.31068317125548	17.250088749992727	11.57129194770831	25.867936131043486
2	16.847518341144514	19.628282518007598	37.45118982793687	26.073009312911015
3	18.555581927845417	23.706788227409806	28.634489370415878	29.103140474328903
4	12.543500493907315	15.487436293325482	35.84762600647917	36.121437206288036
5	14.726985219141383	36.431098873778396	33.46914807504421	15.372767832036017
6	34.130453754719944	35.609806237406204	16.92892785732433	13.33081215054952
7	30.501074704053927	30.471477187607526	20.572213479166724	18.455234629171823
8	28.40471666124816	32.44846146743504	19.231288500170315	19.91553337114649
9	27.46667659976184	14.158540735196487	18.566972536155884	39.80781012888579
10	16.022921208442988	26.407003176559428	31.121978174638592	26.448097440358993
11	37.04258591841359	21.08807743289346	22.58592617426747	19.283410474425477
12	24.919601607385747	23.678980773074155	28.581390881520303	22.820026738019795
13	29.342628902285927	19.424802714937396	25.351329430643165	25.88123895213351
14	23.863933159549404	19.78788891333817	25.074211326381203	31.273966600731228
15	25.20661076847913	27.363310149568466	22.135146745354096	25.294932336598308
16	25.8699557469063	25.725744139268986	23.77011676250374	24.634183351320978
17	24.105386849462214	25.93202290532538	25.143662772016263	24.818927473196144
18	24.865688951058072	24.981505789677552	26.120927683562318	24.03187757570206
19	25.679208031462018	24.741565035956757	25.408964719333905	24.17026221324732
20	26.03527811254917	24.097873570914285	24.76749398867017	25.09935432786637
21	27.15428878371843	23.969303233228207	24.30618678096903	24.57022120208433
22	26.2210786937434	24.099888566780947	24.865482522140123	24.813550217335532
23	24.528088038479574	24.071430345825533	25.46407134495297	25.936410270741924
24	24.72719467830222	24.84274032193648	25.328792755672392	25.10127224408891
25	24.882376731357848	24.439790315807123	25.292088403948693	25.385744548886336
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	166.0
1	166.0
2	462.5
3	759.0
4	759.0
5	759.0
6	2095.0
7	3431.0
8	3431.0
9	3431.0
10	3791.5
11	4152.0
12	4152.0
13	4152.0
14	5217.0
15	6282.0
16	6282.0
17	6282.0
18	11001.5
19	15721.0
20	15721.0
21	15721.0
22	25727.0
23	35733.0
24	35733.0
25	35733.0
26	53876.0
27	72019.0
28	72019.0
29	72019.0
30	91246.0
31	110473.0
32	110473.0
33	110473.0
34	138057.5
35	165642.0
36	165642.0
37	165642.0
38	188755.5
39	211869.0
40	211869.0
41	211869.0
42	242916.5
43	273964.0
44	273964.0
45	273964.0
46	303754.5
47	333545.0
48	333545.0
49	333545.0
50	341228.0
51	348911.0
52	348911.0
53	348911.0
54	322139.0
55	295367.0
56	295367.0
57	295367.0
58	270532.5
59	245698.0
60	245698.0
61	245698.0
62	217512.0
63	189326.0
64	189326.0
65	189326.0
66	157210.0
67	125094.0
68	125094.0
69	125094.0
70	95251.0
71	65408.0
72	65408.0
73	65408.0
74	50674.5
75	35941.0
76	35941.0
77	35941.0
78	28883.0
79	21825.0
80	21825.0
81	21825.0
82	15164.0
83	8503.0
84	8503.0
85	8503.0
86	6570.5
87	4638.0
88	4638.0
89	4638.0
90	3256.5
91	1875.0
92	1875.0
93	1875.0
94	1317.0
95	759.0
96	759.0
97	759.0
98	1082.5
99	1406.0
100	1406.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04041098201401043
2	0.0058561020001109175
3	0.0016676316953958242
4	0.0034128276556937794
5	0.00930771178825576
6	0.013651310622775118
7	0.022803893881226618
8	0.03571834398743149
9	0.04902061541814701
10	0.05836710933885385
11	0.05472158888845367
12	0.0640680828091605
13	0.0640680828091605
14	0.06763603899465855
15	0.06050012662366245
16	0.06740334619995214
17	0.06356391508729664
18	0.07853381821340799
19	0.07523733695506742
20	0.08229568506116136
21	0.075586376147127
22	0.08051170696841234
23	0.08450626661087211
24	0.07896042167036972
25	0.07992997498164635
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2578507.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.32103647435323
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.37502958505569	32.07736326071647
2	14.992622318157265	13.289771212185006
3	5.413890487451992	7.198477132875411
4	2.383960950011264	4.22638480875532
5	1.280899784650901	2.838540303775189
6	0.7568780460136338	2.012737169040448
7	0.48119544616067667	1.4928956644406017
8	0.3549103862577235	1.2583996939564284
9	0.2689792339030701	1.0729294592995402
>10	1.4619898374747615	12.010324452264108
>50	0.11652212005048661	3.5961295288467197
>100	0.08940143078202555	8.227861915346521
>500	0.01590143592430893	4.775368617058604
>1k	0.007731084869276522	5.686855741833751
>5k	8.785323715086957E-5	0.23596103960611386
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6060	0.23501972265345797	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4216	0.16350547041369287	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	3139	0.12173711376389515	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2913	0.1129723518299543	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2853	0.11064542388289036	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2847	0.11041273108818397	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2712	0.1051771432072901	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2687	0.10420758989601346	No Hit
GAACTACGACGGTATCTGATCGTCT	2674	0.10370342217414961	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2663	0.1032768187171879	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.8782132451065675E-5	1.1634639735319702E-4
2	0.0	0.0	0.0	3.8782132451065675E-5	1.1634639735319702E-4
3	0.0	0.0	0.0	3.8782132451065675E-5	1.1634639735319702E-4
4	0.0	0.0	0.0	3.8782132451065675E-5	1.1634639735319702E-4
5	0.0	0.0	0.0	3.8782132451065675E-5	1.1634639735319702E-4
6	0.0	0.0	0.0	3.8782132451065675E-5	1.1634639735319702E-4
7	0.0	0.0	0.0	7.756426490213135E-5	1.1634639735319702E-4
8	0.0	0.0	0.0	7.756426490213135E-5	1.1634639735319702E-4
9	0.0	0.0	0.0	7.756426490213135E-5	1.1634639735319702E-4
10	0.0	0.0	0.0	7.756426490213135E-5	1.551285298042627E-4
11	0.0	0.0	0.0	7.756426490213135E-5	1.551285298042627E-4
12	0.0	0.0	0.0	7.756426490213135E-5	4.2660345696172243E-4
13	0.0	0.0	0.0	7.756426490213135E-5	4.6538558941278807E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGT	70	7.2745006E-6	13.570069	6
CGAACGA	200	0.0	13.301766	16
GTATCAA	4055	0.0	12.04025	1
GTCTAAA	145	9.822543E-11	11.791466	1
CGACCAT	485	0.0	11.7525425	10
CGTCGTA	525	0.0	11.580916	10
ACGAACG	230	0.0	11.566753	15
CCGTCGT	530	0.0	11.471441	9
AGGCCCG	630	0.0	11.460283	10
CGAGCCG	615	0.0	11.432413	15
CGCATCG	275	0.0	11.401071	13
GTATTAG	425	0.0	11.3984165	1
ACCGTCG	555	0.0	11.125876	8
TCGAACG	215	0.0	11.04412	3
CGCTTAC	95	1.3584593E-5	11.001888	18
CGACGCT	95	1.3590439E-5	11.001462	15
CCGACCA	540	0.0	10.907162	9
GTTCAAA	795	0.0	10.872704	1
GTACGCC	140	8.378265E-9	10.855635	4
TCCATTA	570	0.0	10.83309	8
>>END_MODULE