FastQCFastQC Report
Thu 2 Feb 2017
SRR4063961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2578507
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA30810.11948775008173333No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA30390.11785890051878858No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT30270.1173935149293758No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA27680.10734894262454979No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC26580.10308290805493257No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT26240.10176431555159633No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC25830.10017424812110265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44000.022.8005731
GGTATCA21800.020.1839921
CGTCTTA2250.018.57716615
GGCGTTA8050.017.49010542
CGTTATT8200.016.90122444
TAGGACG5000.016.7184734
GTAAACG5450.016.55119527
CGCGGGA5600.016.49881644
TAAACGC5750.016.45258728
AACGCAG60900.016.2907966
GTATTAG6750.015.9707721
GCGTTAT9000.015.64392943
CGCTTCG5950.015.53040432
TAACACG850.002982978215.5279954
CGCAATA8800.015.50159236
ATAAGAC3700.015.4580493
TAGTACG2850.015.4371882
TACGACG8750.015.3357445
TCTATAC3600.015.2763843
AAACGCT6050.015.27340829
Produced by FastQC (version 0.11.5)