##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063961_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2578507
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34682356883266	32.0	32.0	32.0	32.0	32.0
2	31.425837897667137	32.0	32.0	32.0	32.0	32.0
3	31.485942834361126	32.0	32.0	32.0	32.0	32.0
4	31.570051584114374	32.0	32.0	32.0	32.0	32.0
5	31.521728659259022	32.0	32.0	32.0	32.0	32.0
6	35.10562624030107	36.0	36.0	36.0	36.0	36.0
7	35.11688740810089	36.0	36.0	36.0	36.0	36.0
8	35.070846036097635	36.0	36.0	36.0	36.0	36.0
9	35.17772106106363	36.0	36.0	36.0	36.0	36.0
10	35.029052083240416	36.0	36.0	36.0	36.0	36.0
11	35.17287213104328	36.0	36.0	36.0	36.0	36.0
12	35.071267985698704	36.0	36.0	36.0	36.0	36.0
13	35.11785308319892	36.0	36.0	36.0	36.0	36.0
14	35.05781446395142	36.0	36.0	36.0	36.0	36.0
15	35.03230125029717	36.0	36.0	36.0	36.0	36.0
16	35.043861816159506	36.0	36.0	36.0	36.0	36.0
17	35.00758462164345	36.0	36.0	36.0	36.0	36.0
18	35.009356965096465	36.0	36.0	36.0	36.0	36.0
19	35.00696643445219	36.0	36.0	36.0	36.0	36.0
20	34.996620912799536	36.0	36.0	36.0	36.0	36.0
21	34.98789299389143	36.0	36.0	36.0	36.0	36.0
22	34.98053369643751	36.0	36.0	36.0	36.0	36.0
23	34.92303065300967	36.0	36.0	36.0	32.0	36.0
24	34.892450941572	36.0	36.0	36.0	32.0	36.0
25	34.86965907015183	36.0	36.0	36.0	32.0	36.0
26	34.81380892120906	36.0	36.0	36.0	32.0	36.0
27	34.80981164681732	36.0	36.0	36.0	32.0	36.0
28	34.7837388069918	36.0	36.0	36.0	32.0	36.0
29	34.75280617814883	36.0	36.0	36.0	32.0	36.0
30	34.73366409321363	36.0	36.0	36.0	32.0	36.0
31	34.731090898725505	36.0	36.0	36.0	32.0	36.0
32	34.692609715622254	36.0	36.0	36.0	32.0	36.0
33	34.66794234027676	36.0	36.0	36.0	32.0	36.0
34	34.65960689654905	36.0	36.0	36.0	32.0	36.0
35	34.625499562343634	36.0	36.0	36.0	32.0	36.0
36	34.60027217300554	36.0	36.0	36.0	32.0	36.0
37	34.58957761216084	36.0	36.0	36.0	32.0	36.0
38	34.549295386826564	36.0	36.0	36.0	32.0	36.0
39	34.55736672423228	36.0	36.0	36.0	32.0	36.0
40	34.52680485257554	36.0	36.0	36.0	32.0	36.0
41	34.510444222179736	36.0	36.0	36.0	32.0	36.0
42	34.440502973232185	36.0	36.0	36.0	32.0	36.0
43	34.44154000745392	36.0	36.0	36.0	32.0	36.0
44	34.36786830518591	36.0	36.0	36.0	32.0	36.0
45	34.34342896877922	36.0	36.0	36.0	32.0	36.0
46	34.316814342563355	36.0	36.0	36.0	32.0	36.0
47	34.284930388011354	36.0	36.0	36.0	32.0	36.0
48	34.23845814651657	36.0	36.0	36.0	32.0	36.0
49	34.22529083690679	36.0	36.0	36.0	32.0	36.0
50	33.625544937438605	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	2.0
18	4.0
19	12.0
20	33.0
21	90.0
22	284.0
23	844.0
24	2191.0
25	4897.0
26	9797.0
27	17879.0
28	29273.0
29	45578.0
30	65196.0
31	91863.0
32	131968.0
33	209981.0
34	438550.0
35	1530064.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34461352886534	18.13645998374876	12.122066583535828	26.396859903850068
2	16.084376318598128	20.35899997517933	36.93261498175681	26.624008724465735
3	17.815946409131826	24.240808315225255	28.954791140116786	28.988454135526133
4	12.10433014143456	16.114208726212496	35.94250471299865	35.838956419354304
5	14.243979170892304	36.93199204035514	33.204059558496446	15.619969230256114
6	34.165714564945745	35.98296843210759	16.626914965487767	13.224402037458901
7	30.34016248963061	30.60803109401075	20.58578175010849	18.466024666250146
8	28.408067148935412	32.65816226211525	19.088798285209233	19.84497230374011
9	27.525262025034174	13.925944599036253	18.446058232094554	40.10273514383502
10	15.973869937630067	26.67348203558212	31.082270758887194	26.270377267900617
11	37.26937226440427	20.974393699297188	22.50931353272011	19.24692050357843
12	24.836678364677773	23.779785231807164	28.512651342776284	22.87088506073878
13	29.38568935427574	19.31929416327322	25.447779716889663	25.84723676556137
14	23.894338650891097	19.68418097019122	25.033915040593335	31.387565338324347
15	25.299353472515797	27.39837650626942	21.985851636098495	25.316418385116297
16	25.91684652034043	25.743294996868315	23.704074291686787	24.635784191104467
17	24.14389412167584	25.957230288690315	25.066521052686692	24.832354536947157
18	24.883711189554834	24.95662196615544	26.115088885217496	24.04457795907223
19	25.72412086688959	24.71628920002513	25.368682485128986	24.1909074479563
20	26.184133420774724	24.00126585763988	24.81049948244883	25.00410123913656
21	27.21446945848896	23.91798820014838	24.341450304381567	24.52609203698109
22	26.194046000352145	24.05796078498291	24.95421760386457	24.793775610800378
23	24.611371534746485	23.94660287741939	25.53055255961723	25.911473028216893
24	24.867665155321102	24.764969670315693	25.292059676625566	25.075305497737638
25	25.003703764032615	24.270552399922746	25.422132142544108	25.30361169350053
26	24.677934311631134	25.2740505965817	25.67329724832413	24.374717843463046
27	25.42791885201045	24.62170588876379	24.88854310371241	25.061832155513347
28	24.527147152178568	24.630118306293227	25.79216038523235	25.050574156295863
29	24.58536880496549	24.66246980727852	25.587216436630577	25.164944951125413
30	24.609370758034814	24.92297560816691	25.73859643407317	24.7290571997251
31	25.32305263749881	24.589660996899113	24.763186524427965	25.324099841174107
32	25.111812313549187	24.708426995052797	24.580752303341416	25.5990083880566
33	24.649958695475117	24.385532169049917	25.249746937065865	25.714762198409097
34	25.289033818411134	24.57433201288542	25.344532965562607	24.79210120314084
35	25.843594745639788	24.369403086374266	25.32017545560671	24.466826712379238
36	24.613670309502027	25.016803688317466	25.0097447817421	25.359781220438403
37	25.752017921219238	24.817871265701623	24.74022609405227	24.689884719026868
38	24.90408334238329	24.51805079355229	25.303652047101167	25.274213816963247
39	25.55537413361868	24.50937539874581	24.585663107737094	25.34958735989842
40	25.687186366687882	24.751585158364584	25.21279265170651	24.348435823241026
41	24.69667329608037	25.19527553436074	25.44352949571984	24.66452167383905
42	26.043121829990124	25.087978802867756	24.9232682118255	23.94563115531662
43	25.170779305899536	24.315583558337217	25.20285253991314	25.310784595850105
44	24.85142363384206	24.931781303035287	24.6535545082514	25.563240554871257
45	24.93757926212056	25.298490965572608	24.74067955027594	25.023250222030896
46	24.800036921033975	24.806552397617818	24.82671934894876	25.56669133239945
47	25.025170140844192	24.710174942174607	25.263801691867833	25.00085322511336
48	25.85796533195266	25.16774580126471	24.26433658915901	24.709952277623614
49	25.011654071478823	25.259821803235837	24.53098664416261	25.197537481122733
50	25.049612812574296	25.509265776913182	24.44731695228211	24.993804458230414
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	26.0
2	33.0
3	142.5
4	252.0
5	293.0
6	334.0
7	308.0
8	282.0
9	421.5
10	561.0
11	864.5
12	1168.0
13	2194.5
14	3221.0
15	4432.5
16	5644.0
17	6155.0
18	6666.0
19	6778.0
20	6890.0
21	7564.5
22	8239.0
23	9512.0
24	10785.0
25	12741.5
26	14698.0
27	18256.5
28	21815.0
29	26069.5
30	30324.0
31	35006.0
32	39688.0
33	46615.0
34	53542.0
35	61833.0
36	70124.0
37	82694.0
38	95264.0
39	105237.0
40	115210.0
41	125430.0
42	135650.0
43	138563.5
44	141477.0
45	155062.0
46	168647.0
47	179843.5
48	191040.0
49	197189.5
50	203339.0
51	195943.5
52	188548.0
53	185596.0
54	182644.0
55	182945.5
56	183247.0
57	179194.0
58	175141.0
59	158885.5
60	142630.0
61	125294.5
62	107959.0
63	94240.0
64	80521.0
65	68404.0
66	56287.0
67	50267.0
68	44247.0
69	40339.5
70	36432.0
71	28239.0
72	20046.0
73	16983.0
74	13920.0
75	10849.0
76	7778.0
77	6738.5
78	5699.0
79	4763.5
80	3828.0
81	2945.0
82	2062.0
83	1670.0
84	1278.0
85	941.0
86	604.0
87	440.5
88	277.0
89	215.5
90	154.0
91	129.0
92	104.0
93	81.0
94	58.0
95	60.5
96	63.0
97	50.5
98	38.0
99	34.0
100	30.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009385276053157894
2	4.2660345696172243E-4
3	3.102570596085254E-4
4	0.0
5	0.0
6	3.8782132451065675E-5
7	1.551285298042627E-4
8	0.0
9	0.0012410282384341016
10	8.144247814723791E-4
11	3.8782132451065675E-5
12	2.3269279470639404E-4
13	2.714749271574597E-4
14	1.939106622553284E-4
15	0.004537509496774684
16	8.532069139234449E-4
17	0.0
18	5.041677218638538E-4
19	3.8782132451065675E-5
20	6.980783841191821E-4
21	0.0
22	1.939106622553284E-4
23	6.205141192170508E-4
24	3.878213245106568E-4
25	0.0019003244901022183
26	0.0058561020001109175
27	0.006050012662366246
28	0.004459945231872553
29	0.002753531404025663
30	0.003994559642459765
31	0.008221812079625922
32	0.003063788463634188
33	0.00376186684775337
34	0.003451609788144845
35	0.002986224198732057
36	0.007911555020017396
37	0.00430481670206829
38	0.00880354406639191
39	0.004266034569617224
40	0.002249363682161809
41	0.003839431112655502
42	0.0017451959602979553
43	0.0012798103708851671
44	0.0013573746357872986
45	0.00143493890068943
46	0.0012022461059830358
47	0.002133017284808612
48	0.002016670887455415
49	3.4903919205959104E-4
50	0.0015512852980426272
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2578507.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.126813697129464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.33565749021719	34.56075867897962
2	14.698461672755148	13.853833297726592
3	5.089363523897019	7.195364598829832
4	2.302754351254216	4.340859012072467
5	1.2347179979793017	2.9094162529631613
6	0.746931340797614	2.112029648538976
7	0.4829230468426927	1.5931037121023988
8	0.3412125795087235	1.286420933249967
9	0.23929079317301225	1.0149311366372598
>10	1.3290021716619447	11.470693603571682
>50	0.10277573647004166	3.357775286475678
>100	0.07851803892594712	7.695186857181431
>500	0.012426524673853662	4.033533621967687
>1k	0.005964731843448663	4.576093359703321
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	3081	0.11948775008173333	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	3039	0.11785890051878858	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	3027	0.1173935149293758	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	2768	0.10734894262454979	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	2658	0.10308290805493257	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2624	0.10176431555159633	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	2583	0.10017424812110265	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.9391066225532837E-4
2	0.0	0.0	0.0	0.0	1.9391066225532837E-4
3	0.0	0.0	0.0	0.0	1.9391066225532837E-4
4	0.0	0.0	0.0	0.0	1.9391066225532837E-4
5	0.0	0.0	0.0	0.0	2.3269279470639404E-4
6	0.0	0.0	0.0	0.0	3.102570596085254E-4
7	0.0	0.0	0.0	0.0	3.102570596085254E-4
8	0.0	0.0	0.0	0.0	3.102570596085254E-4
9	0.0	0.0	0.0	0.0	3.490391920595911E-4
10	0.0	0.0	0.0	0.0	3.490391920595911E-4
11	0.0	0.0	0.0	0.0	3.490391920595911E-4
12	0.0	0.0	0.0	0.0	6.980783841191822E-4
13	0.0	0.0	0.0	0.0	8.144247814723792E-4
14	0.0	0.0	0.0	3.8782132451065675E-5	8.919890463745105E-4
15	0.0	0.0	0.0	3.8782132451065675E-5	0.0010858997086298388
16	0.0	0.0	0.0	3.8782132451065675E-5	0.0011634639735319703
17	0.0	0.0	0.0	3.8782132451065675E-5	0.0012022461059830358
18	0.0	0.0	0.0	3.8782132451065675E-5	0.0012410282384341016
19	0.0	0.0	0.0	3.8782132451065675E-5	0.0012798103708851673
20	0.0	0.0	0.0	7.756426490213135E-5	0.0013185925033362329
21	0.0	0.0	0.0	1.1634639735319702E-4	0.0013573746357872986
22	0.0	0.0	0.0	1.551285298042627E-4	0.0013961567682383644
23	0.0	0.0	0.0	2.714749271574597E-4	0.0013961567682383644
24	0.0	0.0	0.0	5.817319867659852E-4	0.0013961567682383644
25	0.0	0.0	0.0	6.980783841191822E-4	0.0013961567682383644
26	0.0	0.0	0.0	9.307711788255761E-4	0.001551285298042627
27	0.0	0.0	0.0	0.0010858997086298388	0.001667631695395824
28	0.0	0.0	0.0	0.0020942351523575466	0.001667631695395824
29	0.0	0.0	0.0	0.0038394311126555016	0.0017451959602979555
30	0.0	0.0	0.0	0.007601297960408872	0.0017451959602979555
31	0.0	0.0	0.0	0.01857664144406046	0.0017451959602979555
32	0.0	0.0	0.0	0.029513202795260977	0.0017451959602979555
33	0.0	0.0	0.0	0.04122540679548281	0.0017451959602979555
34	0.0	0.0	0.0	0.05549723153747498	0.0018615423576511523
35	0.0	0.0	0.0	0.07159181650466724	0.0018615423576511523
36	0.0	0.0	0.0	0.09583064928658329	0.0018615423576511523
37	0.0	0.0	0.0	0.13403104975088298	0.001900324490102218
38	0.0	0.0	0.0	0.18770552106315785	0.0019778887550043495
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4400	0.0	22.800573	1
GGTATCA	2180	0.0	20.183992	1
CGTCTTA	225	0.0	18.577166	15
GGCGTTA	805	0.0	17.490105	42
CGTTATT	820	0.0	16.901224	44
TAGGACG	500	0.0	16.718473	4
GTAAACG	545	0.0	16.551195	27
CGCGGGA	560	0.0	16.498816	44
TAAACGC	575	0.0	16.452587	28
AACGCAG	6090	0.0	16.290796	6
GTATTAG	675	0.0	15.970772	1
GCGTTAT	900	0.0	15.643929	43
CGCTTCG	595	0.0	15.530404	32
TAACACG	85	0.0029829782	15.527995	4
CGCAATA	880	0.0	15.501592	36
ATAAGAC	370	0.0	15.458049	3
TAGTACG	285	0.0	15.437188	2
TACGACG	875	0.0	15.335744	5
TCTATAC	360	0.0	15.276384	3
AAACGCT	605	0.0	15.273408	29
>>END_MODULE