FastQCFastQC Report
Thu 2 Feb 2017
SRR4063960_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063960_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909587
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39920.20905043865505996No Hit
TATCAACGCAGAGTACTTTTTTTTT25520.13364146278750327No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTAGAC456.780184E-414.7729263
TAATACG400.00528659914.24718954
CGTTATT1600.013.0551342
TCGCGTA751.4764217E-512.6688179
GTATCAA25700.012.5642971
ATCGCCA2750.012.43782216
CGCATCG2700.012.31496813
GCGTTAT1700.012.2904051
AAGACGG2650.012.18755155
CGAGCCG3600.012.14031215
GGTATCA9300.011.8458821
CGCCAGT2850.011.66864718
CGCGGCG1552.5465852E-1111.64652716
TAATACC1157.095514E-811.5629364
CGGTTCT3000.011.4004412
AGGCCCG4050.011.26028410
TCGCCAG3300.010.94096417
TCTAGAT3150.010.851022
GTATAGG1502.1445885E-910.7634151
GTTATTC1951.8189894E-1210.714433
Produced by FastQC (version 0.11.5)