##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063960_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1909587
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.261580121775022	32.0	32.0	32.0	32.0	32.0
2	30.80918072860781	32.0	32.0	32.0	32.0	32.0
3	30.811408959110008	32.0	32.0	32.0	32.0	32.0
4	30.851021189398544	32.0	32.0	32.0	32.0	32.0
5	30.72991123211459	32.0	32.0	32.0	32.0	32.0
6	34.41314430816716	36.0	36.0	36.0	32.0	36.0
7	34.347495557940015	36.0	36.0	36.0	32.0	36.0
8	34.29497372992171	36.0	36.0	36.0	32.0	36.0
9	34.466492492879354	36.0	36.0	36.0	32.0	36.0
10	34.10791548120091	36.0	36.0	36.0	32.0	36.0
11	34.458637914899924	36.0	36.0	36.0	32.0	36.0
12	34.23803052701972	36.0	36.0	36.0	32.0	36.0
13	34.33704303600726	36.0	36.0	36.0	32.0	36.0
14	34.232597415043145	36.0	36.0	36.0	32.0	36.0
15	34.14277694600979	36.0	36.0	36.0	32.0	36.0
16	34.14979678852024	36.0	36.0	36.0	32.0	36.0
17	34.07018271490118	36.0	36.0	36.0	32.0	36.0
18	34.06398346867673	36.0	36.0	36.0	32.0	36.0
19	34.07939098873212	36.0	36.0	36.0	32.0	36.0
20	34.06225325161933	36.0	36.0	36.0	32.0	36.0
21	34.052903062285196	36.0	36.0	36.0	32.0	36.0
22	34.02462888572241	36.0	36.0	36.0	32.0	36.0
23	33.969120024382235	36.0	36.0	36.0	32.0	36.0
24	33.949219909854854	36.0	36.0	36.0	32.0	36.0
25	33.53754607671711	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	12.0
4	18.0
5	24.0
6	82.0
7	42.0
8	89.0
9	130.0
10	178.0
11	57.0
12	107.0
13	95.0
14	176.0
15	265.0
16	429.0
17	658.0
18	820.0
19	1161.0
20	1843.0
21	2787.0
22	4338.0
23	6873.0
24	10539.0
25	15221.0
26	21824.0
27	29269.0
28	39316.0
29	53705.0
30	70442.0
31	96636.0
32	136805.0
33	193135.0
34	406711.0
35	815800.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.41469366244589	17.090761075409755	11.38063933456651	26.113905927577846
2	16.711504048456842	19.71554753015591	37.72477825265659	25.848170168730654
3	18.826641334266192	23.938332921367614	28.567500932159717	28.667524812206473
4	12.717359654947765	15.70849663892479	35.90610775131605	35.668035954811394
5	14.684902666268638	36.56846879402538	33.689150051586616	15.057478488119367
6	33.9018275159704	35.534456634402005	17.140144125138203	13.423571724489383
7	30.110229835807623	30.443632660800734	20.9363355127175	18.50980199067415
8	28.04735135123808	33.07139949677271	19.411864240740247	19.46938491124896
9	27.765040337749408	14.080174617029769	18.259289104144642	39.895495941076184
10	15.904258384989022	26.742573894271327	31.74689672879322	25.606270991946428
11	37.0138137873796	20.83857297351676	22.81554161112648	19.33207162797716
12	24.953415693409237	23.486193438582077	29.007560386675944	22.55283048133274
13	29.759290952135913	19.583129406265687	25.036392466418846	25.62118717517955
14	23.76024017276173	19.596938206030206	25.296744263066522	31.346077358141542
15	25.174596886600053	27.325513153454523	22.174066598337035	25.325823361608386
16	25.6142761395858	25.63953418298876	23.929271190162147	24.81691848726329
17	24.11691129734168	25.801999280020247	25.214330217089092	24.86675920554898
18	24.69962360581645	25.009669274138858	26.258656489733802	24.032050630310895
19	25.75796403991047	24.705070981583933	25.227508436088797	24.309456542416797
20	25.9527478171546	24.367210674716727	24.862320891376584	24.81772061675209
21	26.80401727739527	24.044916615919647	24.339913151845916	24.81115295483917
22	25.978582782477353	24.332434878630004	24.784614570269447	24.904367768623196
23	24.737460829423043	24.21188458496615	25.27378130304963	25.776873282561176
24	24.92602550194173	24.866699159892878	25.148080017189965	25.059195320975427
25	25.048569028289265	24.480414902922213	25.197459893538575	25.273556175249944
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	118.0
1	118.0
2	312.5
3	507.0
4	507.0
5	507.0
6	1408.0
7	2309.0
8	2309.0
9	2309.0
10	2569.5
11	2830.0
12	2830.0
13	2830.0
14	3707.5
15	4585.0
16	4585.0
17	4585.0
18	7975.0
19	11365.0
20	11365.0
21	11365.0
22	18878.0
23	26391.0
24	26391.0
25	26391.0
26	40024.5
27	53658.0
28	53658.0
29	53658.0
30	69277.5
31	84897.0
32	84897.0
33	84897.0
34	103373.0
35	121849.0
36	121849.0
37	121849.0
38	139396.0
39	156943.0
40	156943.0
41	156943.0
42	179968.0
43	202993.0
44	202993.0
45	202993.0
46	224745.5
47	246498.0
48	246498.0
49	246498.0
50	251921.0
51	257344.0
52	257344.0
53	257344.0
54	241767.5
55	226191.0
56	226191.0
57	226191.0
58	206453.0
59	186715.0
60	186715.0
61	186715.0
62	163465.0
63	140215.0
64	140215.0
65	140215.0
66	114494.5
67	88774.0
68	88774.0
69	88774.0
70	67463.0
71	46152.0
72	46152.0
73	46152.0
74	34979.5
75	23807.0
76	23807.0
77	23807.0
78	19052.5
79	14298.0
80	14298.0
81	14298.0
82	9964.0
83	5630.0
84	5630.0
85	5630.0
86	4222.5
87	2815.0
88	2815.0
89	2815.0
90	2038.0
91	1261.0
92	1261.0
93	1261.0
94	855.5
95	450.0
96	450.0
97	450.0
98	721.0
99	992.0
100	992.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03644767166931907
2	0.004922530369132173
3	0.0022517958071562073
4	0.004137020203845125
5	0.009269019950387179
6	0.014348652352576763
7	0.025398162010947918
8	0.037076079801548714
9	0.04990607916790385
10	0.05849432364170891
11	0.05545701766926566
12	0.06383579276566084
13	0.06414999683177566
14	0.0677633435920961
15	0.06236950712379169
16	0.06723967014857139
17	0.06284081322296392
18	0.07755603698600798
19	0.07415215960309743
20	0.07996493482622159
21	0.07483293507967953
22	0.07834154715129502
23	0.08305460814301731
24	0.07839391449564749
25	0.07739893495295055
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1909587.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.557857517518386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.51837044673485	43.62312437787421
2	12.909979055218903	14.34501553807997
3	3.7465448887990513	6.244500213446533
4	1.5543225765739144	3.4541932898222245
5	0.8269818102477147	2.2972668791660977
6	0.5255754321411326	1.7519906984163118
7	0.34705765894493734	1.3497245976218422
8	0.263575360464189	1.171494585743837
9	0.16989460249383154	0.8495082106512943
>10	0.9944593261410508	9.889823750591766
>50	0.06853592702844091	2.627673242093639
>100	0.06378817462578862	7.331931554627298
>500	0.00844706010587271	3.087575853468631
>1k	0.0024676804803673083	1.9761772083964184
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3992	0.20905043865505996	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2552	0.13364146278750327	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	1.0473468870493987E-4
7	0.0	0.0	0.0	0.0	1.0473468870493987E-4
8	0.0	0.0	0.0	0.0	1.0473468870493987E-4
9	0.0	0.0	0.0	0.0	1.0473468870493987E-4
10	0.0	0.0	0.0	0.0	1.0473468870493987E-4
11	0.0	0.0	0.0	0.0	1.0473468870493987E-4
12	0.0	0.0	0.0	0.0	2.0946937740987974E-4
13	0.0	0.0	0.0	0.0	2.6183672176234964E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTAGAC	45	6.780184E-4	14.772926	3
TAATACG	40	0.005286599	14.2471895	4
CGTTATT	160	0.0	13.055134	2
TCGCGTA	75	1.4764217E-5	12.668817	9
GTATCAA	2570	0.0	12.564297	1
ATCGCCA	275	0.0	12.437822	16
CGCATCG	270	0.0	12.314968	13
GCGTTAT	170	0.0	12.290405	1
AAGACGG	265	0.0	12.1875515	5
CGAGCCG	360	0.0	12.140312	15
GGTATCA	930	0.0	11.845882	1
CGCCAGT	285	0.0	11.668647	18
CGCGGCG	155	2.5465852E-11	11.646527	16
TAATACC	115	7.095514E-8	11.562936	4
CGGTTCT	300	0.0	11.40044	12
AGGCCCG	405	0.0	11.260284	10
TCGCCAG	330	0.0	10.940964	17
TCTAGAT	315	0.0	10.85102	2
GTATAGG	150	2.1445885E-9	10.763415	1
GTTATTC	195	1.8189894E-12	10.71443	3
>>END_MODULE