FastQCFastQC Report
Thu 2 Feb 2017
SRR4063960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909587
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29700.022.5163081
TAGGACG5550.018.2314874
AACGCAG38600.017.3238146
ATACCGT5550.017.0424796
GTAGGAC7400.016.9433943
TGTAGGA8200.016.6316412
ATTGCGA1206.413238E-616.49924510
ATTTCGT2950.016.40817542
GGTATCA15800.016.0110861
TACCGTC5500.015.9980117
ACCGTCG5250.015.9218288
CCGTCGT5350.015.6254519
TATAACG850.002983779915.5270732
TCAACGC43150.015.4461094
TATCAAC44000.015.3976812
TACACCG1004.9512216E-415.397685
AGGACGT6150.015.3797995
ATCAACG43300.015.3418013
GGCGTTA4750.015.28551142
CAACGCA43650.015.2691785
Produced by FastQC (version 0.11.5)