##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063960_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1909587
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34350883201446	32.0	32.0	32.0	32.0	32.0
2	31.437088229025438	32.0	32.0	32.0	32.0	32.0
3	31.505870641138635	32.0	32.0	32.0	32.0	32.0
4	31.578367468986748	32.0	32.0	32.0	32.0	32.0
5	31.54698424318976	32.0	32.0	32.0	32.0	32.0
6	35.13242549305164	36.0	36.0	36.0	36.0	36.0
7	35.15168515495759	36.0	36.0	36.0	36.0	36.0
8	35.097160799691245	36.0	36.0	36.0	36.0	36.0
9	35.21186465974056	36.0	36.0	36.0	36.0	36.0
10	35.064793067820425	36.0	36.0	36.0	36.0	36.0
11	35.20970345943913	36.0	36.0	36.0	36.0	36.0
12	35.11718083543719	36.0	36.0	36.0	36.0	36.0
13	35.15609134331141	36.0	36.0	36.0	36.0	36.0
14	35.10189428394726	36.0	36.0	36.0	36.0	36.0
15	35.07108709893815	36.0	36.0	36.0	36.0	36.0
16	35.08712145610543	36.0	36.0	36.0	36.0	36.0
17	35.05310258186718	36.0	36.0	36.0	36.0	36.0
18	35.05259409495351	36.0	36.0	36.0	36.0	36.0
19	35.05006265752752	36.0	36.0	36.0	36.0	36.0
20	35.0450500553261	36.0	36.0	36.0	36.0	36.0
21	35.03833080137223	36.0	36.0	36.0	36.0	36.0
22	35.01943718720331	36.0	36.0	36.0	36.0	36.0
23	34.96602773269822	36.0	36.0	36.0	36.0	36.0
24	34.946016599400814	36.0	36.0	36.0	36.0	36.0
25	34.92111592716121	36.0	36.0	36.0	36.0	36.0
26	34.86305206309008	36.0	36.0	36.0	32.0	36.0
27	34.85966284856359	36.0	36.0	36.0	32.0	36.0
28	34.836528526849	36.0	36.0	36.0	32.0	36.0
29	34.80163145224596	36.0	36.0	36.0	32.0	36.0
30	34.78524256815741	36.0	36.0	36.0	32.0	36.0
31	34.77765611098106	36.0	36.0	36.0	32.0	36.0
32	34.74076174586442	36.0	36.0	36.0	32.0	36.0
33	34.71061857878169	36.0	36.0	36.0	32.0	36.0
34	34.702798039576095	36.0	36.0	36.0	32.0	36.0
35	34.662263096680064	36.0	36.0	36.0	32.0	36.0
36	34.638733401515616	36.0	36.0	36.0	32.0	36.0
37	34.63263365324544	36.0	36.0	36.0	32.0	36.0
38	34.596786635015846	36.0	36.0	36.0	32.0	36.0
39	34.596574023597775	36.0	36.0	36.0	32.0	36.0
40	34.58663313061934	36.0	36.0	36.0	32.0	36.0
41	34.57225829459459	36.0	36.0	36.0	32.0	36.0
42	34.51578639779177	36.0	36.0	36.0	32.0	36.0
43	34.503826220015114	36.0	36.0	36.0	32.0	36.0
44	34.4475601268756	36.0	36.0	36.0	32.0	36.0
45	34.417744779368526	36.0	36.0	36.0	32.0	36.0
46	34.39161190351631	36.0	36.0	36.0	32.0	36.0
47	34.37006588335593	36.0	36.0	36.0	32.0	36.0
48	34.32375272768405	36.0	36.0	36.0	32.0	36.0
49	34.30293147156951	36.0	36.0	36.0	32.0	36.0
50	33.72326057938182	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	4.0
19	8.0
20	26.0
21	50.0
22	206.0
23	562.0
24	1441.0
25	3349.0
26	6680.0
27	12205.0
28	20542.0
29	31431.0
30	45708.0
31	64824.0
32	94506.0
33	151410.0
34	320668.0
35	1155967.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.58499518700501	17.932988864646433	11.953562716100333	26.528453232248218
2	15.889217408244125	20.433153293554913	37.40601326575819	26.27161603244277
3	18.11735367709515	24.446710215555477	28.933670161679704	28.50226594566967
4	12.302398372004	16.293261317761377	36.03925875071416	35.36508155952046
5	14.185266238197055	37.0620977206066	33.532957650004946	15.219678391191394
6	34.01065256518818	35.85880088207555	16.916537450244476	13.214009102491794
7	30.096481847859895	30.57182147716097	20.85603027259369	18.475666402385443
8	27.994811440804447	33.27517063908093	19.338851457855263	19.39116646225936
9	27.711832054072993	13.948816668909759	18.213972068013323	40.125379209003924
10	15.789539833093139	26.918077806079697	31.792635821295708	25.499746539531454
11	37.188374862404736	20.81000803315483	22.744982420273292	19.256634684167146
12	24.88494876370979	23.511508265674617	29.02841666038258	22.575126310233014
13	29.828940994723958	19.5990730921802	25.094955683856355	25.47703022923949
14	23.71769124463299	19.589617209621156	25.3171503935676	31.37554115217825
15	25.178214565518857	27.351353446144948	22.171033613058107	25.29939837527808
16	25.61704797295316	25.673552662999533	23.960764064902364	24.74863529914494
17	24.102541544323458	25.92759586235139	25.126532595791655	24.8433299975335
18	24.68239155753295	24.996334280136406	26.2863011001349	24.034973062195743
19	25.780810196131416	24.70534204516474	25.23655638627619	24.27729137242765
20	26.063336473641574	24.35756781992261	24.890879089055755	24.68821661738006
21	26.939332955241106	24.062532893238174	24.262680883353312	24.735453268167408
22	26.012301102071916	24.369012115197805	24.83173045452284	24.786956328207438
23	24.793068596595045	24.252266217001733	25.243012824876804	25.711652361526415
24	25.095112786876662	24.7781836272469	25.086472120759858	25.040231465116587
25	25.149708124798053	24.419535712359306	25.287018034101244	25.143738128741393
26	24.75341531700154	25.14032572116266	25.5883520486665	24.517906913169305
27	25.360716977199328	24.76499912802618	24.815798803955673	25.05848509081882
28	24.76750482586559	24.71927235478151	25.4352181877788	25.078004631574096
29	24.833440689593775	24.69817180143805	25.17787099443318	25.29051651453499
30	24.749543076496064	24.992432613602443	25.383475341841628	24.874548968059866
31	25.423358661969285	24.59295508142993	24.64621696597891	25.33746929062188
32	24.997302989962932	24.91236026607706	24.682721644474377	25.407615099485632
33	24.75275005826117	24.521800152395514	25.190245639576748	25.53520414976656
34	25.276154197025342	24.63468778459213	25.39518866150903	24.693969356873495
35	25.63399208285612	24.671711895144714	25.145232300983437	24.549063721015727
36	24.742356587490875	25.05297414610285	25.052240939928417	25.152428326477853
37	25.46782821816011	24.82766566379421	24.791897418978117	24.912608699067565
38	24.952263540866912	24.830971561325246	25.11770454405004	25.099060353757803
39	25.435978004713277	24.533438072793924	24.736370777690496	25.294213144802303
40	25.789117577131222	24.702310192308296	25.054646726066725	24.453925504493757
41	24.879995894251845	24.94797116340354	25.463074974941318	24.708957967403293
42	25.724139989662437	25.213860294175404	24.78789405412086	24.274105662041297
43	24.980780792103683	24.498415331795798	25.296824169908273	25.22397970619225
44	24.804522929116093	25.003574123658918	24.79546324570228	25.396439701522706
45	24.90441500306092	25.196053325457164	24.88488167674492	25.014649994737
46	24.832252020228733	24.678604139904408	24.978411268349113	25.510732571517746
47	25.07429795956201	24.642361698439572	25.27717393865856	25.006166403339858
48	25.430036998342537	25.09325519953706	24.504004880743842	24.97270292137656
49	25.029417509741688	25.2012356663282	24.580522283207223	25.188824540722894
50	24.93963291154487	25.48164060229361	24.5480258089923	25.03070067716922
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	38.0
2	46.0
3	93.0
4	140.0
5	179.5
6	219.0
7	215.0
8	211.0
9	292.5
10	374.0
11	535.5
12	697.0
13	1369.5
14	2042.0
15	2760.0
16	3478.0
17	3789.0
18	4100.0
19	4192.5
20	4285.0
21	4911.5
22	5538.0
23	6437.5
24	7337.0
25	8934.5
26	10532.0
27	13827.0
28	17122.0
29	20651.5
30	24181.0
31	27613.0
32	31045.0
33	36916.5
34	42788.0
35	49704.5
36	56621.0
37	64660.5
38	72700.0
39	78244.0
40	83788.0
41	90354.0
42	96920.0
43	100339.5
44	103759.0
45	113698.0
46	123637.0
47	131545.5
48	139454.0
49	144140.5
50	148827.0
51	146136.5
52	143446.0
53	141923.5
54	140401.0
55	139269.0
56	138137.0
57	133329.0
58	128521.0
59	117329.0
60	106137.0
61	93130.0
62	80123.0
63	69791.5
64	59460.0
65	50110.5
66	40761.0
67	35176.0
68	29591.0
69	26688.0
70	23785.0
71	18752.0
72	13719.0
73	11751.5
74	9784.0
75	7679.0
76	5574.0
77	4834.0
78	4094.0
79	3416.5
80	2739.0
81	2153.5
82	1568.0
83	1281.5
84	995.0
85	712.0
86	429.0
87	303.0
88	177.0
89	139.5
90	102.0
91	83.5
92	65.0
93	47.5
94	30.0
95	35.5
96	41.0
97	35.0
98	29.0
99	18.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009059550572977299
2	4.713060991722294E-4
3	4.713060991722294E-4
4	0.0
5	0.0
6	0.0
7	2.6183672176234964E-4
8	5.236734435246993E-5
9	0.0010473468870493986
10	9.949795426969286E-4
11	5.236734435246993E-5
12	1.5710203305740978E-4
13	6.284081322296391E-4
14	2.094693774098797E-4
15	0.004765428336074764
16	5.760407878771693E-4
17	0.0
18	1.5710203305740978E-4
19	0.0
20	4.189387548197594E-4
21	0.0
22	1.0473468870493986E-4
23	8.902448539919888E-4
24	6.284081322296391E-4
25	0.0019899590853938573
26	0.005289101779599463
27	0.006598285388411212
28	0.004451224269959944
29	0.0029849386280907863
30	0.004032285515140185
31	0.00806457103028037
32	0.0034038773829105457
33	0.004294122236902534
34	0.002932571283738316
35	0.003142040661148196
36	0.00864061181815754
37	0.0037704487933778352
38	0.00759326493110814
39	0.004555958958664884
40	0.0025659998732710265
41	0.0036133467603204255
42	0.0024088978402136167
43	0.002042326429746327
44	0.001466285641869158
45	0.001571020330574098
46	0.0013615509531642183
47	0.0024088978402136167
48	0.0021994284628037374
49	5.236734435246993E-4
50	0.0010473468870493986
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1909587.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.098806145266316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.63411328477171	47.06281023566901
2	12.415922459232567	14.675323890656875
3	3.5484720469205624	6.291313848385641
4	1.459849095363675	3.4510135475296093
5	0.760195447431885	2.2463321690145484
6	0.4909172957641849	1.7407575657435563
7	0.30374899013129963	1.2565841879213062
8	0.22082320448989703	1.0440310203619942
9	0.17333360707344658	0.9219428318604044
>10	0.8757448960949176	9.068640802470114
>50	0.059033345135883354	2.429357174470883
>100	0.05060544574647466	6.282072214768649
>500	0.0055424033863908945	2.2592457320128116
>1k	0.0016984784571197902	1.2705747791345086
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0473468870493987E-4
2	0.0	0.0	0.0	0.0	1.0473468870493987E-4
3	0.0	0.0	0.0	0.0	1.0473468870493987E-4
4	0.0	0.0	0.0	0.0	1.0473468870493987E-4
5	0.0	0.0	0.0	0.0	1.0473468870493987E-4
6	0.0	0.0	0.0	0.0	1.5710203305740978E-4
7	0.0	0.0	0.0	0.0	1.5710203305740978E-4
8	0.0	0.0	0.0	0.0	2.0946937740987974E-4
9	0.0	0.0	0.0	0.0	2.0946937740987974E-4
10	0.0	0.0	0.0	5.2367344352469935E-5	2.0946937740987974E-4
11	0.0	0.0	0.0	5.2367344352469935E-5	2.0946937740987974E-4
12	0.0	0.0	0.0	5.2367344352469935E-5	4.713060991722294E-4
13	0.0	0.0	0.0	5.2367344352469935E-5	5.236734435246993E-4
14	0.0	0.0	0.0	5.2367344352469935E-5	5.236734435246993E-4
15	0.0	0.0	0.0	5.2367344352469935E-5	5.760407878771692E-4
16	0.0	0.0	0.0	1.0473468870493987E-4	6.284081322296391E-4
17	0.0	0.0	0.0	1.0473468870493987E-4	6.807754765821091E-4
18	0.0	0.0	0.0	1.5710203305740978E-4	6.807754765821091E-4
19	0.0	0.0	0.0	1.5710203305740978E-4	6.807754765821091E-4
20	0.0	0.0	0.0	1.5710203305740978E-4	8.37877509639519E-4
21	0.0	0.0	0.0	1.5710203305740978E-4	9.949795426969286E-4
22	0.0	0.0	0.0	3.1420406611481957E-4	0.0010473468870493986
23	0.0	0.0	0.0	5.760407878771692E-4	0.0010473468870493986
24	0.0	0.0	0.0	8.902448539919889E-4	0.0010473468870493986
25	0.0	0.0	0.0	0.0014662856418691582	0.0010473468870493986
26	0.0	0.0	0.0	0.0017281223636315078	0.0010473468870493986
27	0.0	0.0	0.0	0.0024612651845660866	0.0010473468870493986
28	0.0	0.0	0.0	0.004032285515140185	0.0010473468870493986
29	0.0	0.0	0.0	0.0068077547658210915	0.0010473468870493986
30	0.0	0.0	0.0	0.013091836088117483	0.0010473468870493986
31	0.0	0.0	0.0	0.02953518221479304	0.0010997142314018685
32	0.0	0.0	0.0	0.04812558945991987	0.0011520815757543384
33	0.0	0.0	0.0	0.06703020077116151	0.0011520815757543384
34	0.0	0.0	0.0	0.08918158743225629	0.0013615509531642181
35	0.0	0.0	0.0	0.11248505566910541	0.0013615509531642181
36	0.0	0.0	0.0	0.14568595198857134	0.0013615509531642181
37	0.0	0.0	0.0	0.20020035745949255	0.0013615509531642181
38	0.0	0.0	0.0	0.27246729266590103	0.0014139182975166883
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2970	0.0	22.516308	1
TAGGACG	555	0.0	18.231487	4
AACGCAG	3860	0.0	17.323814	6
ATACCGT	555	0.0	17.042479	6
GTAGGAC	740	0.0	16.943394	3
TGTAGGA	820	0.0	16.631641	2
ATTGCGA	120	6.413238E-6	16.499245	10
ATTTCGT	295	0.0	16.408175	42
GGTATCA	1580	0.0	16.011086	1
TACCGTC	550	0.0	15.998011	7
ACCGTCG	525	0.0	15.921828	8
CCGTCGT	535	0.0	15.625451	9
TATAACG	85	0.0029837799	15.527073	2
TCAACGC	4315	0.0	15.446109	4
TATCAAC	4400	0.0	15.397681	2
TACACCG	100	4.9512216E-4	15.39768	5
AGGACGT	615	0.0	15.379799	5
ATCAACG	4330	0.0	15.341801	3
GGCGTTA	475	0.0	15.285511	42
CAACGCA	4365	0.0	15.269178	5
>>END_MODULE