FastQCFastQC Report
Thu 2 Feb 2017
SRR4063959_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063959_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207028
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT88650.4016713879479553No Hit
CTGTAGGACGTGGAATATGGCAAGA86460.39174854147749827No Hit
GTCCTACAGTGGACATTTCTAAATT82990.37602604044896576No Hit
CTTTAGGACGTGAAATATGGCGAGG74850.33914386224370513No Hit
GTCCTACAGTGTGCATTTCTCATTT53500.2424074366070571No Hit
CTGTAGGACCTGGAATATGGCGAGA37600.17036485264346443No Hit
CTGAAGGACCTGGAATATGGCGAGA37460.16973051542617493No Hit
ATTTAGAAATGTCCACTGTAGGACG36690.1662416607310827No Hit
GTCCTTCAGTGTGCATTTCTCATTT33960.15387208499393756No Hit
GTATCAACGCAGAGTACTTTTTTTT27460.124420714191211No Hit
TTTCTAAATTTTCCACCTTTTTCAG26850.1216568163158782No Hit
GAATATGGCAAGAAAACTGAAAATC24720.11200582865283086No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGC250.006025089419.00424214
GCTCGTA801.2885903E-714.2505959
GTATCAA22550.013.9828411
TAGAAAT10600.013.3500934
GGTATCA6700.013.1829321
ATTTAGA11000.012.6919551
CGGTCCA2250.012.66748410
TAGGACC16800.012.6066434
AAATGTC10500.012.576437
TTTAGAA12050.012.5284062
GCGTTAT1301.9826984E-1012.4196521
TTAGAAA11250.012.3232563
CCGATAA853.943587E-612.2946319
AGGACCT25300.012.20074755
CGCCGGT2350.012.1278927
ACCTTTT8150.012.12568215
TAAGACG1103.8313374E-812.0875774
AAGGCGA951.0379008E-612.00240717
CCGTCGT1750.011.94335659
GAAATGT12150.011.8836536
Produced by FastQC (version 0.11.5)