##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063959_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2207028
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.341145649262266	32.0	32.0	32.0	32.0	32.0
2	31.010158004338866	32.0	32.0	32.0	32.0	32.0
3	31.011468816888595	32.0	32.0	32.0	32.0	32.0
4	31.038511971755682	32.0	32.0	32.0	32.0	32.0
5	30.987136094331383	32.0	32.0	32.0	32.0	32.0
6	34.66376094911347	36.0	36.0	36.0	32.0	36.0
7	34.610326647419065	36.0	36.0	36.0	32.0	36.0
8	34.58050147075615	36.0	36.0	36.0	32.0	36.0
9	34.6873347325	36.0	36.0	36.0	32.0	36.0
10	34.43072675108789	36.0	36.0	36.0	32.0	36.0
11	34.64334933675513	36.0	36.0	36.0	32.0	36.0
12	34.496558720596205	36.0	36.0	36.0	32.0	36.0
13	34.54941124444275	36.0	36.0	36.0	32.0	36.0
14	34.476474698100795	36.0	36.0	36.0	32.0	36.0
15	34.43111460298646	36.0	36.0	36.0	32.0	36.0
16	34.425118756989036	36.0	36.0	36.0	32.0	36.0
17	34.38087600157316	36.0	36.0	36.0	32.0	36.0
18	34.37959328110019	36.0	36.0	36.0	32.0	36.0
19	34.35219172570534	36.0	36.0	36.0	32.0	36.0
20	34.32416534815145	36.0	36.0	36.0	32.0	36.0
21	34.313763123983925	36.0	36.0	36.0	32.0	36.0
22	34.275182734428384	36.0	36.0	36.0	32.0	36.0
23	34.24362128618214	36.0	36.0	36.0	32.0	36.0
24	34.21377617320668	36.0	36.0	36.0	32.0	36.0
25	33.85115866223718	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	23.0
5	32.0
6	82.0
7	37.0
8	146.0
9	153.0
10	216.0
11	60.0
12	141.0
13	106.0
14	171.0
15	321.0
16	559.0
17	687.0
18	916.0
19	1207.0
20	1757.0
21	2596.0
22	3956.0
23	6246.0
24	9345.0
25	13739.0
26	20365.0
27	27419.0
28	37838.0
29	51949.0
30	69624.0
31	95754.0
32	136879.0
33	197947.0
34	444524.0
35	1082230.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.402214682812186	17.600563869303798	11.373298250146746	26.62392319773727
2	14.909271005237207	19.25300715663858	41.67597836242842	24.161743475695793
3	19.274424193653626	23.48816851171187	28.82846879372467	28.408938500909837
4	11.954068508463532	15.762646815340162	37.08756789381129	35.19571678238501
5	13.24186532742377	37.73870897432005	35.192600752485816	13.82682494577036
6	33.57829553616542	36.68496670420327	17.139665614584445	12.597072145046868
7	29.68456899313973	30.35391776831169	21.074583736125483	18.886929502423097
8	25.763939378006306	37.94232244097986	18.986572362564342	17.307165818449494
9	27.155375047203616	14.017196850764938	18.83212466379463	39.99530343823681
10	15.553709820890191	27.67016701379979	33.42932765588574	23.34679550942428
11	36.50787102249621	20.066018391031797	25.06998756431489	18.3561230221571
12	25.634644236955612	22.850502396932797	31.053077110368633	20.461776255742958
13	30.717447921389684	20.827136737410633	24.948447344652852	23.506967996546834
14	21.5259458493805	21.822344181170703	25.49769256369821	31.15401740575059
15	23.06860171470283	32.59164048367133	21.44784029959694	22.8919175020289
16	22.881332108497705	25.97559880768962	28.34933750962382	22.793731574188854
17	22.09015789533342	28.097253550661392	27.34035342257688	22.472235131428313
18	22.50376038385164	25.38981247735494	30.70404996855199	21.402377170241433
19	25.606797052023094	24.50259804861813	25.56634920548549	24.324255693873283
20	25.719258403365153	25.915341458415337	26.383646201593695	21.98175393662581
21	24.515783239937715	24.937129134445254	24.74346056842699	25.80362705719004
22	25.251261465653712	27.71183938856498	25.11648745926057	21.92041168652074
23	22.614535881674033	27.414918631623582	26.922740290100883	23.047805196601505
24	25.018070874104282	25.33101215940824	27.327424285985074	22.32349268050241
25	23.510285891787838	26.56247378361649	27.298006307803586	22.62923401679209
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	71.0
2	235.5
3	400.0
4	400.0
5	400.0
6	940.0
7	1480.0
8	1480.0
9	1480.0
10	2055.0
11	2630.0
12	2630.0
13	2630.0
14	4005.0
15	5380.0
16	5380.0
17	5380.0
18	9723.0
19	14066.0
20	14066.0
21	14066.0
22	25710.0
23	37354.0
24	37354.0
25	37354.0
26	62673.5
27	87993.0
28	87993.0
29	87993.0
30	122795.0
31	157597.0
32	157597.0
33	157597.0
34	175566.0
35	193535.0
36	193535.0
37	193535.0
38	207436.0
39	221337.0
40	221337.0
41	221337.0
42	238057.0
43	254777.0
44	254777.0
45	254777.0
46	269698.5
47	284620.0
48	284620.0
49	284620.0
50	276606.0
51	268592.0
52	268592.0
53	268592.0
54	240823.0
55	213054.0
56	213054.0
57	213054.0
58	193155.5
59	173257.0
60	173257.0
61	173257.0
62	150219.0
63	127181.0
64	127181.0
65	127181.0
66	104358.0
67	81535.0
68	81535.0
69	81535.0
70	61594.0
71	41653.0
72	41653.0
73	41653.0
74	31327.0
75	21001.0
76	21001.0
77	21001.0
78	16067.0
79	11133.0
80	11133.0
81	11133.0
82	7632.5
83	4132.0
84	4132.0
85	4132.0
86	3081.0
87	2030.0
88	2030.0
89	2030.0
90	1435.5
91	841.0
92	841.0
93	841.0
94	588.0
95	335.0
96	335.0
97	335.0
98	689.5
99	1044.0
100	1044.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038196162441074606
2	0.005709034955605457
3	0.0016311528444587018
4	0.004032572309911791
5	0.009469748458107463
6	0.013275771761844435
7	0.02514693968540499
8	0.037697754627490004
9	0.05251405963132321
10	0.060805753257321606
11	0.05795123577951888
12	0.06801001165368087
13	0.06868965867220535
14	0.0702301919141941
15	0.0633431021264796
16	0.07077390952901368
17	0.0658351411944026
18	0.08160295202417006
19	0.07788754832290302
20	0.08337003427233365
21	0.0776609993167282
22	0.0840496812908581
23	0.08758384578718531
24	0.0825544578501043
25	0.08259976765133926
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2207028.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.442323404633655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.44626429013644	36.807962752997554
2	14.691483593034626	14.527621662013745
3	4.800180026813012	7.11996159858456
4	2.113504275929164	4.179862477102632
5	1.0897703950873643	2.694039015535242
6	0.6375637089642893	1.8913578653802068
7	0.42591335008397907	1.474070191804213
8	0.29106833278360306	1.1512875713867547
9	0.19842008335232758	0.8829314937972131
>10	1.1218604014808065	10.0221748172125
>50	0.09445853399013397	3.24499515444143
>100	0.07620414409780461	7.783371895198369
>500	0.008077103818513249	2.6813228167188083
>1k	0.004772834074575999	3.785300817265784
>5k	4.589263533246153E-4	1.7537398705610254
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	8865	0.4016713879479553	No Hit
CTGTAGGACGTGGAATATGGCAAGA	8646	0.39174854147749827	No Hit
GTCCTACAGTGGACATTTCTAAATT	8299	0.37602604044896576	No Hit
CTTTAGGACGTGAAATATGGCGAGG	7485	0.33914386224370513	No Hit
GTCCTACAGTGTGCATTTCTCATTT	5350	0.2424074366070571	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3760	0.17036485264346443	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3746	0.16973051542617493	No Hit
ATTTAGAAATGTCCACTGTAGGACG	3669	0.1662416607310827	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	3396	0.15387208499393756	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2746	0.124420714191211	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2685	0.1216568163158782	No Hit
GAATATGGCAAGAAAACTGAAAATC	2472	0.11200582865283086	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3592940370489183E-4
2	0.0	0.0	0.0	0.0	1.3592940370489183E-4
3	0.0	0.0	0.0	0.0	1.3592940370489183E-4
4	0.0	0.0	0.0	0.0	1.3592940370489183E-4
5	0.0	0.0	0.0	0.0	1.3592940370489183E-4
6	0.0	0.0	0.0	0.0	2.265490061748197E-4
7	0.0	0.0	0.0	0.0	2.265490061748197E-4
8	0.0	0.0	0.0	4.5309801234963945E-5	2.265490061748197E-4
9	0.0	0.0	0.0	4.5309801234963945E-5	2.7185880740978366E-4
10	0.0	0.0	0.0	4.5309801234963945E-5	2.7185880740978366E-4
11	0.0	0.0	0.0	4.5309801234963945E-5	2.7185880740978366E-4
12	0.0	0.0	0.0	9.061960246992789E-5	9.968156271692068E-4
13	0.0	0.0	0.0	9.061960246992789E-5	0.0011327450308740985
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACGC	25	0.0060250894	19.004242	14
GCTCGTA	80	1.2885903E-7	14.250595	9
GTATCAA	2255	0.0	13.982841	1
TAGAAAT	1060	0.0	13.350093	4
GGTATCA	670	0.0	13.182932	1
ATTTAGA	1100	0.0	12.691955	1
CGGTCCA	225	0.0	12.667484	10
TAGGACC	1680	0.0	12.606643	4
AAATGTC	1050	0.0	12.57643	7
TTTAGAA	1205	0.0	12.528406	2
GCGTTAT	130	1.9826984E-10	12.419652	1
TTAGAAA	1125	0.0	12.323256	3
CCGATAA	85	3.943587E-6	12.294631	9
AGGACCT	2530	0.0	12.2007475	5
CGCCGGT	235	0.0	12.127892	7
ACCTTTT	815	0.0	12.125682	15
TAAGACG	110	3.8313374E-8	12.087577	4
AAGGCGA	95	1.0379008E-6	12.002407	17
CCGTCGT	175	0.0	11.9433565	9
GAAATGT	1215	0.0	11.883653	6
>>END_MODULE