FastQCFastQC Report
Thu 2 Feb 2017
SRR4063959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207028
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA45730.20720172104749013No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA33320.15097225771489986No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA31880.14444764633706506No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC30510.138240203567875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA24450.029.0623191
TAGGACC23650.023.5324124
GGTATCA10200.023.2932531
TGTAGGA54950.022.4175192
TTAGGAC33850.021.7697453
GTAGGAC54950.021.6569253
CTGTAGG55250.021.6209181
AGGACCT38150.021.2192655
TAAGACG1259.986252E-1021.117784
TAGGACG58950.020.8222394
AGGACGT58850.020.5585865
CTTTAGG34900.020.4863471
GGACGTG58650.020.1786146
GTCCTAC45800.020.125891
TTTAGGA36600.019.9537372
TACCGTC3650.019.8883237
GACGTGA32800.019.8515747
GGACCTG39400.019.6527586
ACCGTCG3300.019.3313038
GTCCTAA27700.019.1400831
Produced by FastQC (version 0.11.5)