FastQCFastQC Report
Thu 2 Feb 2017
SRR4063958_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063958_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412981
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25700.18188496519061473No Hit
TATCAACGCAGAGTACTTTTTTTTT17210.12179923155371515No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA602.5649982E-514.25172717
GGTATCA6850.014.0055241
TCGCGTA1101.8553692E-1013.8193679
GCGTTAT1051.373337E-913.5697081
CGAACGA1001.0051735E-813.30161116
TCCAACG1950.013.15637218
TCGACGC751.47651535E-512.66820114
ATAAGCC905.428392E-712.6619223
GGTCGGC1600.012.469377511
CCAACGA1405.2750693E-1112.21663119
GATTTAG701.09205575E-412.2127371
GCGTAAC1251.4078978E-912.16061311
CGGAAAA550.00306623612.09194712
TAGGGCC550.0030743812.0876654
AGGACGT3400.012.0119075
AACCGCG951.0389049E-612.0006057
AAGAATT3100.011.95306116
GTATCAA18500.011.91200351
CCAACGT802.8602983E-511.8772819
CGACGCT802.8613355E-511.8768615
Produced by FastQC (version 0.11.5)