##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063958_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1412981
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.267319235007406	32.0	32.0	32.0	32.0	32.0
2	30.858675382046894	32.0	32.0	32.0	32.0	32.0
3	30.873809343508512	32.0	32.0	32.0	32.0	32.0
4	30.90794426818195	32.0	32.0	32.0	32.0	32.0
5	30.80871504995467	32.0	32.0	32.0	32.0	32.0
6	34.47385279773755	36.0	36.0	36.0	32.0	36.0
7	34.42304673594337	36.0	36.0	36.0	32.0	36.0
8	34.375094923427845	36.0	36.0	36.0	32.0	36.0
9	34.52626893072164	36.0	36.0	36.0	32.0	36.0
10	34.212046729573856	36.0	36.0	36.0	32.0	36.0
11	34.487696579076434	36.0	36.0	36.0	32.0	36.0
12	34.299180243754165	36.0	36.0	36.0	32.0	36.0
13	34.37761866578531	36.0	36.0	36.0	32.0	36.0
14	34.28361386317297	36.0	36.0	36.0	32.0	36.0
15	34.22654090890111	36.0	36.0	36.0	32.0	36.0
16	34.21674742972482	36.0	36.0	36.0	32.0	36.0
17	34.14649170795644	36.0	36.0	36.0	32.0	36.0
18	34.14394744161457	36.0	36.0	36.0	32.0	36.0
19	34.16148200152727	36.0	36.0	36.0	32.0	36.0
20	34.12348219827443	36.0	36.0	36.0	32.0	36.0
21	34.115609480948436	36.0	36.0	36.0	32.0	36.0
22	34.09200194482445	36.0	36.0	36.0	32.0	36.0
23	34.02076956448813	36.0	36.0	36.0	32.0	36.0
24	34.020841044571725	36.0	36.0	36.0	32.0	36.0
25	33.603914702320836	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	11.0
5	15.0
6	45.0
7	27.0
8	80.0
9	80.0
10	141.0
11	39.0
12	85.0
13	69.0
14	142.0
15	254.0
16	421.0
17	544.0
18	727.0
19	973.0
20	1517.0
21	2119.0
22	3332.0
23	5029.0
24	7460.0
25	10559.0
26	15348.0
27	20523.0
28	28152.0
29	37755.0
30	50076.0
31	67934.0
32	95531.0
33	136479.0
34	291392.0
35	636120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.48041278570882	16.97593971334955	11.5242854270446	26.019362073897025
2	16.598261598250982	19.359731390671854	38.173920859617795	25.86808615145937
3	19.588395700087545	22.91481918442078	28.34795631012648	29.148828805365195
4	12.600341135088081	15.519523260175664	35.97701230775764	35.90312329697862
5	14.615630160149088	36.40035077512384	33.83415754162977	15.149861523097297
6	34.278516886889406	35.43634963462938	16.841235776593063	13.44389770188815
7	30.296635815031763	30.21855699293893	20.226237457306173	19.25856973472313
8	27.799111352765767	33.60321249656633	19.128075079080542	19.46960107158736
9	27.090900851527238	14.354103913796498	18.252818480109553	40.30217675456671
10	15.860052487044651	26.923474568709732	31.657364119312426	25.559108824933187
11	37.5043902881162	20.994145338363754	22.133425104517183	19.368039269002864
12	24.49517232197362	23.399614465612796	29.216481382598264	22.88873182981532
13	29.946751667595567	19.50635347006472	24.932315342870677	25.614579519469032
14	23.374808693749234	19.6939383273265	25.0613490197967	31.869903959127566
15	25.0610071310715	27.540594704453557	22.108446106236677	25.289952058238264
16	25.61171489801093	25.76447542304821	24.098486275631828	24.52532340330903
17	23.976902223947015	25.852569677158016	25.211248056064655	24.95928004283031
18	24.529244028166737	25.400140805367037	26.27103758256733	23.79957758389889
19	25.60003739609834	24.73751905217889	25.12422870547173	24.53821484625104
20	25.648795054425122	24.657753525875307	25.01175763963259	24.681693780066976
21	26.726614424858486	24.25065302260223	24.361851014522315	24.660881538016966
22	25.791394982838412	24.470951373847807	25.176325932767373	24.561327710546408
23	24.723904827203235	24.484428742229955	25.159013253701573	25.63265317686524
24	24.655037842784232	24.96908408138288	25.33993345109477	25.035944624738114
25	25.019353860820775	24.621723212521328	25.23077664050974	25.128146286148162
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	63.0
1	63.0
2	188.5
3	314.0
4	314.0
5	314.0
6	778.0
7	1242.0
8	1242.0
9	1242.0
10	1529.5
11	1817.0
12	1817.0
13	1817.0
14	2500.5
15	3184.0
16	3184.0
17	3184.0
18	5716.5
19	8249.0
20	8249.0
21	8249.0
22	13756.0
23	19263.0
24	19263.0
25	19263.0
26	29747.0
27	40231.0
28	40231.0
29	40231.0
30	52936.5
31	65642.0
32	65642.0
33	65642.0
34	78413.0
35	91184.0
36	91184.0
37	91184.0
38	104440.0
39	117696.0
40	117696.0
41	117696.0
42	133958.0
43	150220.0
44	150220.0
45	150220.0
46	164749.0
47	179278.0
48	179278.0
49	179278.0
50	184695.0
51	190112.0
52	190112.0
53	190112.0
54	177060.0
55	164008.0
56	164008.0
57	164008.0
58	150496.5
59	136985.0
60	136985.0
61	136985.0
62	119996.5
63	103008.0
64	103008.0
65	103008.0
66	85522.0
67	68036.0
68	68036.0
69	68036.0
70	51618.5
71	35201.0
72	35201.0
73	35201.0
74	27017.5
75	18834.0
76	18834.0
77	18834.0
78	14767.5
79	10701.0
80	10701.0
81	10701.0
82	7343.5
83	3986.0
84	3986.0
85	3986.0
86	2966.5
87	1947.0
88	1947.0
89	1947.0
90	1340.0
91	733.0
92	733.0
93	733.0
94	532.5
95	332.0
96	332.0
97	332.0
98	523.5
99	715.0
100	715.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03743857843806817
2	0.005237154639729763
3	0.0015569919199196593
4	0.0036093903598137553
5	0.007784959599598296
6	0.011960528839382837
7	0.021656342158882534
8	0.03517386291818503
9	0.049186790197461965
10	0.058245652276994526
11	0.05470703427717711
12	0.06475670939665855
13	0.0649690264766476
14	0.06921536807642849
15	0.06022727835689227
16	0.06843687211646865
17	0.06348280691672428
18	0.078061913075972
19	0.07565565283609617
20	0.08018508387586246
21	0.07678801059603774
22	0.0783450025159574
23	0.08131744163580402
24	0.07692955531603043
25	0.07912349847591722
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1412981.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.10716549666831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.59788003159535	38.34976915010278
2	15.140001280330154	15.778051046678666
3	5.103160772567845	7.977337287968951
4	2.2373601032107038	4.663299726945721
5	1.1671994604246696	3.0409727725985105
6	0.638725910343697	1.9969318030373482
7	0.4141347710462478	1.510557233698217
8	0.28860558223002164	1.2030735069217642
9	0.221788277956144	1.0401082654213678
>10	1.0321917655966841	9.566831027629043
>50	0.07792532421922546	2.8554812817672985
>100	0.07079612700627055	7.688563220617753
>500	0.007775251039563694	2.7241468094549566
>1k	0.0024553424335464293	1.6048768671576334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2570	0.18188496519061473	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1721	0.12179923155371515	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.077235999634814E-5	0.0
10	0.0	0.0	0.0	7.077235999634814E-5	0.0
11	0.0	0.0	0.0	2.1231707998904445E-4	0.0
12	0.0	0.0	0.0	2.1231707998904445E-4	7.077235999634814E-5
13	0.0	0.0	0.0	2.8308943998539256E-4	1.4154471999269628E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTTA	60	2.5649982E-5	14.251727	17
GGTATCA	685	0.0	14.005524	1
TCGCGTA	110	1.8553692E-10	13.819367	9
GCGTTAT	105	1.373337E-9	13.569708	1
CGAACGA	100	1.0051735E-8	13.301611	16
TCCAACG	195	0.0	13.156372	18
TCGACGC	75	1.47651535E-5	12.668201	14
ATAAGCC	90	5.428392E-7	12.661922	3
GGTCGGC	160	0.0	12.4693775	11
CCAACGA	140	5.2750693E-11	12.216631	19
GATTTAG	70	1.09205575E-4	12.212737	1
GCGTAAC	125	1.4078978E-9	12.160613	11
CGGAAAA	55	0.003066236	12.091947	12
TAGGGCC	55	0.00307438	12.087665	4
AGGACGT	340	0.0	12.011907	5
AACCGCG	95	1.0389049E-6	12.000605	7
AAGAATT	310	0.0	11.953061	16
GTATCAA	1850	0.0	11.9120035	1
CCAACGT	80	2.8602983E-5	11.87728	19
CGACGCT	80	2.8613355E-5	11.87686	15
>>END_MODULE