FastQCFastQC Report
Thu 2 Feb 2017
SRR4063958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412981
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19950.025.031011
GTAGGAC5350.022.203433
CCGTCGT4200.020.9518079
GATATAC7250.020.9366171
CGTCGTA4100.020.92625610
GGTATCA8850.020.6314741
TACCGTC4400.020.4972637
TAGGACG4550.020.3056984
ACTGTTC8300.020.141898
ACCGTCG4150.020.141898
AGTATCG550.00448652619.9980375
ATACCGT4450.019.7733426
GTCGTAG4500.019.06614511
TCTATAC1504.656613E-1019.0647963
ATACACT8050.018.8552914
CTATACC1801.4551915E-1118.3315354
TACGAAT3450.017.85458441
ATACGAA3500.017.59889640
ACGCTTA750.001291866217.59827417
TATACAC9750.017.5982743
Produced by FastQC (version 0.11.5)