##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063958_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1412981
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.316782745132453	32.0	32.0	32.0	32.0	32.0
2	31.38002704919599	32.0	32.0	32.0	32.0	32.0
3	31.469818065494156	32.0	32.0	32.0	32.0	32.0
4	31.561887244060607	32.0	32.0	32.0	32.0	32.0
5	31.499493623764227	32.0	32.0	32.0	32.0	32.0
6	35.093309110313584	36.0	36.0	36.0	36.0	36.0
7	35.120070970522605	36.0	36.0	36.0	36.0	36.0
8	35.0542852310116	36.0	36.0	36.0	36.0	36.0
9	35.17020398717322	36.0	36.0	36.0	36.0	36.0
10	35.02258629096924	36.0	36.0	36.0	36.0	36.0
11	35.17287493603948	36.0	36.0	36.0	36.0	36.0
12	35.07393729993539	36.0	36.0	36.0	36.0	36.0
13	35.12441710114998	36.0	36.0	36.0	36.0	36.0
14	35.057615070549424	36.0	36.0	36.0	36.0	36.0
15	35.02850639888293	36.0	36.0	36.0	36.0	36.0
16	35.04747834542715	36.0	36.0	36.0	36.0	36.0
17	35.00913317305753	36.0	36.0	36.0	36.0	36.0
18	35.01413324029127	36.0	36.0	36.0	36.0	36.0
19	35.01259040284335	36.0	36.0	36.0	36.0	36.0
20	34.99266939895158	36.0	36.0	36.0	36.0	36.0
21	34.99941046624123	36.0	36.0	36.0	36.0	36.0
22	34.98155035347255	36.0	36.0	36.0	36.0	36.0
23	34.922415800354	36.0	36.0	36.0	32.0	36.0
24	34.90038861102874	36.0	36.0	36.0	32.0	36.0
25	34.86687719084687	36.0	36.0	36.0	32.0	36.0
26	34.81056220855057	36.0	36.0	36.0	32.0	36.0
27	34.80105323426147	36.0	36.0	36.0	32.0	36.0
28	34.778551162400625	36.0	36.0	36.0	32.0	36.0
29	34.75302357214994	36.0	36.0	36.0	32.0	36.0
30	34.731892360902236	36.0	36.0	36.0	32.0	36.0
31	34.72726455628207	36.0	36.0	36.0	32.0	36.0
32	34.688660357074866	36.0	36.0	36.0	32.0	36.0
33	34.668315426746716	36.0	36.0	36.0	32.0	36.0
34	34.66566217097045	36.0	36.0	36.0	32.0	36.0
35	34.62769704617401	36.0	36.0	36.0	32.0	36.0
36	34.599905448127046	36.0	36.0	36.0	32.0	36.0
37	34.59177158079267	36.0	36.0	36.0	32.0	36.0
38	34.54911637169926	36.0	36.0	36.0	32.0	36.0
39	34.5465296419414	36.0	36.0	36.0	32.0	36.0
40	34.53052871906983	36.0	36.0	36.0	32.0	36.0
41	34.518081276393666	36.0	36.0	36.0	32.0	36.0
42	34.45875563790313	36.0	36.0	36.0	32.0	36.0
43	34.44908601035683	36.0	36.0	36.0	32.0	36.0
44	34.38232219683067	36.0	36.0	36.0	32.0	36.0
45	34.36161915836094	36.0	36.0	36.0	32.0	36.0
46	34.33223801310846	36.0	36.0	36.0	32.0	36.0
47	34.31295749907465	36.0	36.0	36.0	32.0	36.0
48	34.25658023710156	36.0	36.0	36.0	32.0	36.0
49	34.24710381809805	36.0	36.0	36.0	32.0	36.0
50	33.63460230533885	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	1.0
19	7.0
20	17.0
21	43.0
22	153.0
23	438.0
24	1070.0
25	2520.0
26	5246.0
27	9426.0
28	15620.0
29	24092.0
30	34893.0
31	50254.0
32	73097.0
33	118680.0
34	248712.0
35	828711.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.61434373261498	17.843174170862667	12.094243283453208	26.448238813069146
2	15.842754360660564	20.152387800483236	37.72737502600897	26.277482812847232
3	18.831317729388186	23.600259310844628	28.702327569611942	28.866095390155245
4	12.30405787480511	16.179764625285124	35.970830464103905	35.54534703580586
5	14.2849054587429	36.83779187405917	33.54036607710932	15.336936590088616
6	34.36635248390279	35.811173280829564	16.51214668593566	13.31032754933198
7	30.16298886042265	30.457118996730316	20.181814321504906	19.198077821342128
8	27.897402725160493	33.87717173833194	18.827570929828497	19.397854606679072
9	27.089129343955882	14.189412506573037	18.122973681658973	40.5984844678121
10	15.742111640178688	27.155179735139086	31.66087271615493	25.4418359085273
11	37.67467503101599	20.878978556682647	22.10737440913926	19.33897200316211
12	24.487978943777648	23.47317263738527	29.157475093401953	22.881373325435128
13	30.013878538372634	19.38550842797421	25.010244357110857	25.590368676542298
14	23.400401987289275	19.634955908788516	25.049965321519057	31.914676782403145
15	25.10991390902837	27.61776288038139	22.0349431391922	25.237380071398036
16	25.589578741901743	25.828014703904856	24.001916546705566	24.58049000748784
17	24.050288008119004	25.91768749898265	25.1143504406641	24.917674052234247
18	24.620499838284697	25.372458291960875	26.190022909077783	23.81701896067664
19	25.650097205836452	24.7907084384008	25.0717454799463	24.487448875816447
20	25.824713229382525	24.596808438359886	24.949043720487822	24.629434611769767
21	26.83022630877556	24.265082120707923	24.352061351143433	24.552630219373086
22	25.870783733669267	24.466305255559174	25.10198304293054	24.560927967841018
23	24.771403174028002	24.388875048833377	25.23029537823928	25.609426398899338
24	24.851518642973815	24.847130728705167	25.265327267631633	25.036023360689384
25	25.137353401827795	24.42976133099215	25.324619717033947	25.108265550146115
26	24.379990105603923	25.55663055471608	25.6975443071481	24.365835032531898
27	25.137889862219843	24.688248237847894	25.274488057566586	24.899373842365684
28	24.51379162204876	25.187359820740408	25.46791297190293	24.830935585307902
29	24.562583070644905	24.60299486041301	25.365369290153822	25.46905277878827
30	24.49330433447116	25.24599608331346	25.422013420348794	24.83868616186659
31	25.154543151052394	24.844324405431763	24.73037227665029	25.270760166865553
32	25.116991099334147	25.018330728812792	24.678539883136295	25.186138288716762
33	24.727813712257884	24.637432931019717	25.262454606128763	25.37229875059363
34	25.148625847875074	24.976502961193084	25.42464527964307	24.45022591128877
35	25.326855092414018	24.797531379716688	25.520353024024462	24.355260503844832
36	24.455432369994565	25.303284675665882	25.100292738720782	25.140990215618768
37	25.343558885748845	25.01813605901491	24.93164917465892	24.706655880577326
38	24.75940662244906	24.90924443381196	25.09744412224557	25.233904821493407
39	25.515985975203233	24.518481505754725	24.918219354729125	25.047313164312925
40	25.718687829849035	24.9434342304826	25.04280031168677	24.295077627981602
41	24.509287686861352	24.99465655446139	26.026118478696286	24.46993727998098
42	25.710728822160146	25.27030307491201	25.141919288071563	23.877048814856284
43	24.778125353867058	24.428080058883477	25.60744819386253	25.186346393386934
44	24.451172603464908	25.194855930817916	25.037456642653368	25.316514823063805
45	24.660396186755555	25.337339080093702	24.967409074502644	25.034855658648098
46	24.47592585253169	24.850952038545813	25.00113236817819	25.671989740744305
47	24.84696986532499	24.96084452676196	25.380390202957344	24.811795404955706
48	25.339624631181273	24.956881207283732	24.595794493392226	25.107699668142768
49	24.771210329382107	25.306887080416622	25.031299120510635	24.890603469690635
50	24.998885321130135	25.414819779428054	24.517060956302466	25.069233943139345
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	9.0
2	12.0
3	38.0
4	64.0
5	88.5
6	113.0
7	107.0
8	101.0
9	149.5
10	198.0
11	313.0
12	428.0
13	816.5
14	1205.0
15	1652.5
16	2100.0
17	2339.0
18	2578.0
19	2654.0
20	2730.0
21	3276.5
22	3823.0
23	4607.0
24	5391.0
25	6828.5
26	8266.0
27	11402.5
28	14539.0
29	17138.5
30	19738.0
31	22206.0
32	24674.0
33	29287.5
34	33901.0
35	39556.0
36	45211.0
37	50554.5
38	55898.0
39	59468.0
40	63038.0
41	67254.0
42	71470.0
43	74883.0
44	78296.0
45	84474.0
46	90652.0
47	96134.0
48	101616.0
49	105427.5
50	109239.0
51	105809.5
52	102380.0
53	100784.5
54	99189.0
55	99021.5
56	98854.0
57	95704.0
58	92554.0
59	85116.5
60	77679.0
61	68777.0
62	59875.0
63	52250.5
64	44626.0
65	38325.5
66	32025.0
67	28115.0
68	24205.0
69	21360.5
70	18516.0
71	14223.0
72	9930.0
73	8449.5
74	6969.0
75	5492.5
76	4016.0
77	3430.0
78	2844.0
79	2370.0
80	1896.0
81	1426.5
82	957.0
83	782.0
84	607.0
85	431.0
86	255.0
87	186.5
88	118.0
89	100.0
90	82.0
91	58.5
92	35.0
93	26.0
94	17.0
95	19.5
96	22.0
97	22.5
98	23.0
99	21.5
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008988089719536213
2	4.95406519974437E-4
3	3.538617999817407E-4
4	0.0
5	0.0
6	2.1231707998904445E-4
7	7.077235999634814E-5
8	0.0
9	8.492683199561778E-4
10	9.200406799525258E-4
11	0.0
12	1.4154471999269628E-4
13	5.661788799707851E-4
14	7.077235999634814E-5
15	0.004034024519791845
16	0.0016277642799160076
17	0.0
18	2.8308943998539256E-4
19	0.0
20	3.538617999817407E-4
21	0.0
22	7.077235999634814E-5
23	9.200406799525258E-4
24	6.369512399671334E-4
25	0.0014154471999269628
26	0.004246341599780889
27	0.005520244079715155
28	0.004104796879788193
29	0.0019108537199014
30	0.0036801627198101033
31	0.0075726425196092515
32	0.0037509350798064518
33	0.00495406519974437
34	0.0026185773198648815
35	0.003255528559832015
36	0.008280366119572733
37	0.003538617999817407
38	0.008492683199561778
39	0.003892479799799148
40	0.0016985366399123556
41	0.002477032599872185
42	0.0021231707998904444
43	0.001486219559923311
44	9.90813039948874E-4
45	7.784959599598297E-4
46	9.200406799525258E-4
47	0.0016985366399123556
48	0.0016985366399123556
49	1.4154471999269628E-4
50	0.0012739024799342667
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1412981.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.18565052007042
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.32475843722243	41.01660144105212
2	14.872643390250879	16.415130008880404
3	4.991778211686673	8.264235835915283
4	2.1682936749727832	4.786347878877086
5	1.111093097003898	3.0658197673259906
6	0.6302588976318438	2.0868748357125466
7	0.3960256258123345	1.529845224815017
8	0.2761821360350604	1.2193032671293913
9	0.1887192986103331	0.9373137533552387
>10	0.9135384696228498	8.833949258805331
>50	0.06286881061932811	2.39231809719175
>100	0.05687564811631465	6.474347096322679
>500	0.00554564821964592	2.0948096141198382
>1k	0.0014186541957233749	0.8831039204973653
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.077235999634814E-5
2	0.0	0.0	0.0	0.0	7.077235999634814E-5
3	0.0	0.0	0.0	0.0	7.077235999634814E-5
4	0.0	0.0	0.0	0.0	7.077235999634814E-5
5	0.0	0.0	0.0	0.0	7.077235999634814E-5
6	0.0	0.0	0.0	0.0	1.4154471999269628E-4
7	0.0	0.0	0.0	0.0	1.4154471999269628E-4
8	0.0	0.0	0.0	0.0	1.4154471999269628E-4
9	0.0	0.0	0.0	0.0	1.4154471999269628E-4
10	0.0	0.0	0.0	0.0	2.1231707998904445E-4
11	0.0	0.0	0.0	7.077235999634814E-5	2.8308943998539256E-4
12	0.0	0.0	0.0	7.077235999634814E-5	2.8308943998539256E-4
13	0.0	0.0	0.0	7.077235999634814E-5	2.8308943998539256E-4
14	0.0	0.0	0.0	7.077235999634814E-5	2.8308943998539256E-4
15	0.0	0.0	0.0	1.4154471999269628E-4	2.8308943998539256E-4
16	0.0	0.0	0.0	2.8308943998539256E-4	2.8308943998539256E-4
17	0.0	0.0	0.0	2.8308943998539256E-4	2.8308943998539256E-4
18	0.0	0.0	0.0	2.8308943998539256E-4	2.8308943998539256E-4
19	0.0	0.0	0.0	2.8308943998539256E-4	2.8308943998539256E-4
20	0.0	0.0	0.0	4.246341599780889E-4	2.8308943998539256E-4
21	0.0	0.0	0.0	6.369512399671333E-4	2.8308943998539256E-4
22	0.0	0.0	0.0	0.0010615853999452222	2.8308943998539256E-4
23	0.0	0.0	0.0	0.001486219559923311	2.8308943998539256E-4
24	0.0	0.0	0.0	0.0017693089999087036	2.8308943998539256E-4
25	0.0	0.0	0.0	0.0022647155198831405	2.8308943998539256E-4
26	0.0	0.0	0.0	0.002547804959868533	2.8308943998539256E-4
27	0.0	0.0	0.0	0.0031847561998356666	2.8308943998539256E-4
28	0.0	0.0	0.0	0.00460020339976263	2.8308943998539256E-4
29	0.0	0.0	0.0	0.008351138479569082	2.8308943998539256E-4
30	0.0	0.0	0.0	0.014791423239236762	2.8308943998539256E-4
31	0.0	0.0	0.0	0.03050288715842605	2.8308943998539256E-4
32	0.0	0.0	0.0	0.050248375597407184	2.8308943998539256E-4
33	0.0	0.0	0.0	0.06935691279642119	2.8308943998539256E-4
34	0.0	0.0	0.0	0.09341951519517955	3.5386179998174076E-4
35	0.0	0.0	0.0	0.11918065423385028	3.5386179998174076E-4
36	0.0	0.0	0.0	0.15753927335187098	3.5386179998174076E-4
37	0.0	0.0	0.0	0.2155018361888801	3.5386179998174076E-4
38	0.0	0.0	0.0	0.29101594430498356	3.5386179998174076E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1995	0.0	25.03101	1
GTAGGAC	535	0.0	22.20343	3
CCGTCGT	420	0.0	20.951807	9
GATATAC	725	0.0	20.936617	1
CGTCGTA	410	0.0	20.926256	10
GGTATCA	885	0.0	20.631474	1
TACCGTC	440	0.0	20.497263	7
TAGGACG	455	0.0	20.305698	4
ACTGTTC	830	0.0	20.14189	8
ACCGTCG	415	0.0	20.14189	8
AGTATCG	55	0.004486526	19.998037	5
ATACCGT	445	0.0	19.773342	6
GTCGTAG	450	0.0	19.066145	11
TCTATAC	150	4.656613E-10	19.064796	3
ATACACT	805	0.0	18.855291	4
CTATACC	180	1.4551915E-11	18.331535	4
TACGAAT	345	0.0	17.854584	41
ATACGAA	350	0.0	17.598896	40
ACGCTTA	75	0.0012918662	17.598274	17
TATACAC	975	0.0	17.598274	3
>>END_MODULE