FastQCFastQC Report
Thu 2 Feb 2017
SRR4063957_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063957_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100987
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38910.35341016742250364No Hit
TATCAACGCAGAGTACTTTTTTTTT27500.2497758829123323No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22920.20817684495820568No Hit
GAGTACTTTTTTTTTTTTTTTTTTT15700.14259932224449517No Hit
ACGCAGAGTACTTTTTTTTTTTTTT14930.13560559752294987No Hit
GGTATCAACGCAGAGTACTTTTTTT13310.12089152732956883No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11910.10817566419948645No Hit
GTACATGGGGTGGTATCAACGCAAA11240.10209021541580418No Hit
GAATAGGACCGCGGTTCTATTTTGT11210.10181773263444527No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCCGT307.717165E-419.00131810
TTAGACG350.002171946716.2831444
TCTAGAC953.0559022E-1014.9949053
TAGACGG456.7635434E-414.7767885
CTAGACA851.7182174E-814.5271184
TAGACAG1450.014.41282755
CGCGCTA400.005272195714.25228516
CCGTCTA400.00527537714.2509889
GTAATAC602.5751498E-514.2451613
GTCTTAG602.5784624E-514.2432191
GTCTTAC551.9672603E-413.8116051
CGTCGTA1950.012.66754410
CCGTCGT2000.012.3508579
CATCTAG701.0951973E-412.2084741
TTGGACT1251.4115358E-912.158084
GGGTAGG2350.012.1218891
AATCGCT550.003063785412.09284815
CAAATCG550.003065872512.09174813
CAAGACG2750.012.0890014
ATCCCCG1501.4551915E-1112.03416912
Produced by FastQC (version 0.11.5)