##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063957_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1100987
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.246191826061526	32.0	32.0	32.0	32.0	32.0
2	31.021864926652178	32.0	32.0	32.0	32.0	32.0
3	31.031993111635288	32.0	32.0	32.0	32.0	32.0
4	31.051665460173464	32.0	32.0	32.0	32.0	32.0
5	31.017093753150583	32.0	32.0	32.0	32.0	32.0
6	34.58398873011216	36.0	36.0	36.0	32.0	36.0
7	34.53252036581722	36.0	36.0	36.0	32.0	36.0
8	34.49501765234285	36.0	36.0	36.0	32.0	36.0
9	34.589606416787845	36.0	36.0	36.0	32.0	36.0
10	34.407480742279425	36.0	36.0	36.0	32.0	36.0
11	34.568027597056094	36.0	36.0	36.0	32.0	36.0
12	34.463505018678696	36.0	36.0	36.0	32.0	36.0
13	34.51547475129134	36.0	36.0	36.0	32.0	36.0
14	34.4531061674661	36.0	36.0	36.0	32.0	36.0
15	34.39297285072394	36.0	36.0	36.0	32.0	36.0
16	34.396982888989605	36.0	36.0	36.0	32.0	36.0
17	34.33568607077105	36.0	36.0	36.0	32.0	36.0
18	34.32884947778675	36.0	36.0	36.0	32.0	36.0
19	34.325591491997635	36.0	36.0	36.0	32.0	36.0
20	34.29129226775611	36.0	36.0	36.0	32.0	36.0
21	34.264328279988774	36.0	36.0	36.0	32.0	36.0
22	34.24849158073619	36.0	36.0	36.0	32.0	36.0
23	34.204437472922024	36.0	36.0	36.0	32.0	36.0
24	34.19391600445782	36.0	36.0	36.0	32.0	36.0
25	33.732501836988085	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	14.0
5	8.0
6	37.0
7	15.0
8	61.0
9	63.0
10	112.0
11	34.0
12	70.0
13	61.0
14	138.0
15	246.0
16	377.0
17	526.0
18	676.0
19	959.0
20	1227.0
21	1662.0
22	2223.0
23	3229.0
24	4618.0
25	6623.0
26	9344.0
27	12531.0
28	17608.0
29	24639.0
30	33508.0
31	48672.0
32	73535.0
33	109854.0
34	252167.0
35	496148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.34435333798558	16.86129768550826	10.919508897418003	25.874840079088163
2	16.847909021545615	19.392598917269193	37.58283980670712	26.17665225447807
3	19.09857425336091	24.140868890711527	28.238539393097163	28.5220174628304
4	12.358191168750011	14.994177776728183	35.658074724284575	36.989556330237235
5	14.24067305176874	36.895946574426645	33.79374618492573	15.069634188878878
6	34.54866362720545	35.328876789180754	16.77217575825292	13.35028382536088
7	30.328811576086167	30.118682500690436	20.951949212901724	18.600556710321673
8	28.474793409079552	33.026199226788904	19.14583342797825	19.353173936153297
9	27.753262732425952	13.4827499095831	18.219777583515224	40.54420977447572
10	15.622162909324878	26.719066794259067	31.92327370842228	25.735496587993772
11	37.58734601700345	20.565630495335636	22.536249335447785	19.310774152213128
12	24.965917394671116	23.294324655447582	29.34457786648344	22.395180083397864
13	30.181952030828235	19.27210099155677	25.13682756364231	25.409119413972682
14	23.499172922763712	19.3615145511061	25.320197044334975	31.81911548179521
15	24.940722059391543	27.509872083248133	22.42728284824938	25.122123009110947
16	25.305788579166933	25.361593063520772	24.48462650076799	24.8479918565443
17	23.78813987559666	25.796879373838944	25.51904403558328	24.895936714981115
18	24.6585889068664	24.553602268802152	27.01447088552366	23.77333793880779
19	25.725585153715613	24.411255966063766	25.411818600757513	24.451340279463107
20	25.84667266595766	24.00343595756865	25.368547353494584	24.781344022979102
21	26.667278689955058	23.824642602638125	24.600518462500546	24.907560244906268
22	25.97966275477231	24.129367931071155	25.02415581889362	24.866813495262914
23	24.303316501606666	24.260502406588465	25.784266047331844	25.65191504447303
24	25.122960617152767	24.647752259012186	25.45500654911289	24.77428057472215
25	24.792505756028035	24.397832661152876	25.605394168618673	25.204267414200416
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	104.0
1	104.0
2	308.5
3	513.0
4	513.0
5	513.0
6	1671.0
7	2829.0
8	2829.0
9	2829.0
10	2763.5
11	2698.0
12	2698.0
13	2698.0
14	2559.0
15	2420.0
16	2420.0
17	2420.0
18	4387.5
19	6355.0
20	6355.0
21	6355.0
22	10715.0
23	15075.0
24	15075.0
25	15075.0
26	23193.0
27	31311.0
28	31311.0
29	31311.0
30	39917.5
31	48524.0
32	48524.0
33	48524.0
34	58310.5
35	68097.0
36	68097.0
37	68097.0
38	78022.0
39	87947.0
40	87947.0
41	87947.0
42	100502.5
43	113058.0
44	113058.0
45	113058.0
46	128027.5
47	142997.0
48	142997.0
49	142997.0
50	145335.5
51	147674.0
52	147674.0
53	147674.0
54	138206.0
55	128738.0
56	128738.0
57	128738.0
58	119143.0
59	109548.0
60	109548.0
61	109548.0
62	96501.5
63	83455.0
64	83455.0
65	83455.0
66	68622.5
67	53790.0
68	53790.0
69	53790.0
70	40446.5
71	27103.0
72	27103.0
73	27103.0
74	20682.0
75	14261.0
76	14261.0
77	14261.0
78	11381.5
79	8502.0
80	8502.0
81	8502.0
82	5771.5
83	3041.0
84	3041.0
85	3041.0
86	2331.0
87	1621.0
88	1621.0
89	1621.0
90	1094.0
91	567.0
92	567.0
93	567.0
94	380.0
95	193.0
96	193.0
97	193.0
98	379.5
99	566.0
100	566.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.040236624047332074
2	0.006085448783682278
3	0.0018165518757260529
4	0.0029973105949479876
5	0.008265311034553541
6	0.012170897567364556
7	0.021344484539781123
8	0.034696140826367614
9	0.0480477971129541
10	0.055223177022072016
11	0.056494763335080245
12	0.06530503993235161
13	0.06330683286905295
14	0.06603166068264203
15	0.06012786708653236
16	0.065123384744779
17	0.06312517768148035
18	0.07693097193699834
19	0.07320704059175993
20	0.07783924787486136
21	0.07366117856069146
22	0.07611352359292163
23	0.08010993771951894
24	0.07611352359292163
25	0.07593186840534902
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1100987.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.76542141744002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.03459800134092	34.64032106753832
2	15.64858519630595	15.26219703369146
3	5.858586254402	8.570892828190049
4	2.7368595608971513	5.3385643938999765
5	1.412304729431904	3.44358176502952
6	0.8580701858611397	2.5106492531555733
7	0.526875984107899	1.7985330579825087
8	0.3493275725782501	1.3628085031607784
9	0.2518369759414646	1.1052842634251303
>10	1.1393844558162658	9.773704881886093
>50	0.08396130099366396	2.908347723316038
>100	0.0897234192944622	8.90234729620958
>500	0.007647941211119509	2.5745725658240506
>1k	0.0022384218178886366	1.808195366690827
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3891	0.35341016742250364	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2750	0.2497758829123323	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2292	0.20817684495820568	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1570	0.14259932224449517	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1493	0.13560559752294987	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1331	0.12089152732956883	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1191	0.10817566419948645	No Hit
GTACATGGGGTGGTATCAACGCAAA	1124	0.10209021541580418	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1121	0.10181773263444527	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7248278135890797E-4
2	0.0	0.0	0.0	0.0	2.7248278135890797E-4
3	0.0	0.0	0.0	0.0	2.7248278135890797E-4
4	0.0	0.0	0.0	0.0	2.7248278135890797E-4
5	0.0	0.0	0.0	0.0	2.7248278135890797E-4
6	0.0	0.0	0.0	0.0	5.449655627178159E-4
7	0.0	0.0	0.0	0.0	5.449655627178159E-4
8	0.0	0.0	0.0	0.0	5.449655627178159E-4
9	0.0	0.0	0.0	0.0	5.449655627178159E-4
10	0.0	0.0	0.0	9.082759378630266E-5	5.449655627178159E-4
11	0.0	0.0	0.0	9.082759378630266E-5	5.449655627178159E-4
12	0.0	0.0	0.0	9.082759378630266E-5	0.0014532415005808425
13	0.0	0.0	0.0	9.082759378630266E-5	0.001816551875726053
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCCGT	30	7.717165E-4	19.001318	10
TTAGACG	35	0.0021719467	16.283144	4
TCTAGAC	95	3.0559022E-10	14.994905	3
TAGACGG	45	6.7635434E-4	14.776788	5
CTAGACA	85	1.7182174E-8	14.527118	4
TAGACAG	145	0.0	14.4128275	5
CGCGCTA	40	0.0052721957	14.252285	16
CCGTCTA	40	0.005275377	14.250988	9
GTAATAC	60	2.5751498E-5	14.245161	3
GTCTTAG	60	2.5784624E-5	14.243219	1
GTCTTAC	55	1.9672603E-4	13.811605	1
CGTCGTA	195	0.0	12.667544	10
CCGTCGT	200	0.0	12.350857	9
CATCTAG	70	1.0951973E-4	12.208474	1
TTGGACT	125	1.4115358E-9	12.15808	4
GGGTAGG	235	0.0	12.121889	1
AATCGCT	55	0.0030637854	12.092848	15
CAAATCG	55	0.0030658725	12.091748	13
CAAGACG	275	0.0	12.089001	4
ATCCCCG	150	1.4551915E-11	12.034169	12
>>END_MODULE