FastQCFastQC Report
Thu 2 Feb 2017
SRR4063957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1100987
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA11310.1027260085723083No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11060.10045531872765073No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGA508.3306964E-526.40468833
TAGACAG2250.021.5090685
GTATATG1259.968062E-1021.1179921
CGTATAT2250.020.5332510
CGCAATC755.472241E-520.53324944
ATTAGAC755.4760698E-520.5313823
TGTAGGA7850.020.4566562
GTAGGAC7500.020.2380773
ATAGCGT2300.020.0850496
GTATAGA1001.1242737E-619.798121
TAGCGTA2450.019.7532277
GTCCTAC5950.019.5947781
TCCTACA6300.019.2045252
GGCGTTA2300.019.13035442
TATGGCG7850.019.05551516
GATATAC2200.018.9981961
AATCGTA708.12292E-418.85706513
AGCGTAT2450.018.8553528
AATCACG3550.018.59400634
GCGAGAA5100.018.54894420
Produced by FastQC (version 0.11.5)